Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_875 and Pf1N1B4_876 are separated by 12 nucleotides Pf1N1B4_876 and Pf1N1B4_877 are separated by 248 nucleotides
Pf1N1B4_875 - Glutamate synthase [NADPH] small chain (EC 1.4.1.13), at 1,000,743 to 1,001,546
_875
Pf1N1B4_876 - Magnesium and cobalt efflux protein CorC, at 1,001,559 to 1,002,803
_876
Pf1N1B4_877 - Permeases of the major facilitator superfamily, at 1,003,052 to 1,003,864
_877
Position (kb)
1001
1002
1003 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1000.580 kb on - strand at 1000.624 kb on - strand at 1000.624 kb on - strand at 1000.624 kb on - strand at 1000.692 kb on + strand at 1000.692 kb on + strand at 1000.692 kb on + strand at 1000.693 kb on - strand at 1000.724 kb on - strand at 1000.733 kb on + strand at 1000.733 kb on + strand at 1000.733 kb on + strand at 1000.734 kb on - strand at 1000.734 kb on - strand at 1000.734 kb on - strand at 1000.760 kb on + strand at 1000.783 kb on + strand at 1000.783 kb on + strand at 1000.783 kb on + strand at 1000.783 kb on + strand at 1000.783 kb on + strand at 1000.783 kb on + strand at 1000.783 kb on + strand at 1000.784 kb on - strand at 1000.784 kb on - strand at 1000.784 kb on - strand at 1000.784 kb on - strand at 1000.784 kb on - strand at 1000.784 kb on - strand at 1000.784 kb on - strand at 1000.784 kb on - strand at 1000.784 kb on - strand at 1000.785 kb on + strand at 1000.786 kb on - strand at 1000.786 kb on - strand at 1000.905 kb on + strand, within Pf1N1B4_875 at 1000.906 kb on - strand, within Pf1N1B4_875 at 1000.906 kb on - strand, within Pf1N1B4_875 at 1001.027 kb on - strand, within Pf1N1B4_875 at 1001.372 kb on + strand, within Pf1N1B4_875 at 1001.372 kb on + strand at 1001.373 kb on - strand, within Pf1N1B4_875 at 1001.373 kb on - strand at 1001.530 kb on - strand at 1001.612 kb on - strand at 1001.762 kb on - strand, within Pf1N1B4_876 at 1001.863 kb on - strand, within Pf1N1B4_876 at 1001.932 kb on + strand, within Pf1N1B4_876 at 1002.216 kb on - strand, within Pf1N1B4_876 at 1002.298 kb on + strand, within Pf1N1B4_876 at 1002.299 kb on - strand at 1002.299 kb on - strand, within Pf1N1B4_876 at 1002.299 kb on - strand, within Pf1N1B4_876 at 1002.354 kb on + strand, within Pf1N1B4_876 at 1002.355 kb on - strand, within Pf1N1B4_876 at 1002.431 kb on - strand, within Pf1N1B4_876 at 1002.809 kb on + strand at 1002.810 kb on - strand at 1002.810 kb on - strand at 1002.814 kb on - strand at 1002.814 kb on - strand at 1002.814 kb on - strand at 1002.829 kb on - strand at 1002.829 kb on - strand at 1002.848 kb on + strand at 1002.849 kb on - strand at 1002.849 kb on - strand at 1002.849 kb on - strand at 1002.849 kb on - strand at 1002.849 kb on - strand at 1002.849 kb on - strand at 1002.892 kb on - strand at 1002.892 kb on - strand at 1002.957 kb on + strand at 1002.975 kb on + strand at 1002.976 kb on - strand at 1003.007 kb on + strand at 1003.007 kb on + strand at 1003.010 kb on + strand at 1003.010 kb on + strand at 1003.010 kb on + strand at 1003.010 kb on + strand at 1003.010 kb on + strand at 1003.010 kb on + strand at 1003.010 kb on + strand at 1003.010 kb on + strand at 1003.011 kb on - strand at 1003.011 kb on - strand at 1003.011 kb on - strand at 1003.011 kb on - strand at 1003.011 kb on - strand at 1003.011 kb on - strand at 1003.154 kb on + strand, within Pf1N1B4_877 at 1003.154 kb on + strand, within Pf1N1B4_877 at 1003.154 kb on + strand, within Pf1N1B4_877 at 1003.163 kb on + strand, within Pf1N1B4_877 at 1003.163 kb on + strand, within Pf1N1B4_877 at 1003.163 kb on + strand at 1003.164 kb on - strand, within Pf1N1B4_877 at 1003.164 kb on - strand, within Pf1N1B4_877 at 1003.164 kb on - strand, within Pf1N1B4_877 at 1003.169 kb on + strand, within Pf1N1B4_877 at 1003.169 kb on + strand, within Pf1N1B4_877 at 1003.169 kb on + strand, within Pf1N1B4_877 at 1003.170 kb on - strand, within Pf1N1B4_877 at 1003.170 kb on - strand, within Pf1N1B4_877 at 1003.397 kb on + strand, within Pf1N1B4_877 at 1003.398 kb on - strand, within Pf1N1B4_877 at 1003.688 kb on + strand, within Pf1N1B4_877 at 1003.688 kb on + strand, within Pf1N1B4_877 at 1003.688 kb on + strand, within Pf1N1B4_877 at 1003.688 kb on + strand, within Pf1N1B4_877 at 1003.688 kb on + strand, within Pf1N1B4_877 at 1003.688 kb on + strand, within Pf1N1B4_877 at 1003.688 kb on + strand, within Pf1N1B4_877 at 1003.688 kb on + strand at 1003.688 kb on + strand at 1003.688 kb on + strand, within Pf1N1B4_877 at 1003.689 kb on - strand, within Pf1N1B4_877 at 1003.689 kb on - strand, within Pf1N1B4_877 at 1003.689 kb on - strand, within Pf1N1B4_877 at 1003.689 kb on - strand, within Pf1N1B4_877 at 1003.689 kb on - strand, within Pf1N1B4_877
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 1,000,580 - -0.3 1,000,624 - -0.0 1,000,624 - -0.1 1,000,624 - -2.6 1,000,692 + +1.7 1,000,692 + +0.5 1,000,692 + -0.7 1,000,693 - -0.1 1,000,724 - -1.3 1,000,733 + +0.1 1,000,733 + +0.2 1,000,733 + -1.7 1,000,734 - +2.2 1,000,734 - -0.9 1,000,734 - -0.1 1,000,760 + +1.9 1,000,783 + -0.1 1,000,783 + +0.5 1,000,783 + +0.7 1,000,783 + +0.3 1,000,783 + -0.4 1,000,783 + -0.7 1,000,783 + -0.9 1,000,784 - -2.4 1,000,784 - -0.3 1,000,784 - -1.2 1,000,784 - -0.5 1,000,784 - +1.6 1,000,784 - +1.3 1,000,784 - -0.3 1,000,784 - +0.6 1,000,784 - -1.4 1,000,785 + -0.0 1,000,786 - -0.7 1,000,786 - +0.4 1,000,905 + Pf1N1B4_875 0.20 -1.9 1,000,906 - Pf1N1B4_875 0.20 -1.9 1,000,906 - Pf1N1B4_875 0.20 -1.8 1,001,027 - Pf1N1B4_875 0.35 -2.3 1,001,372 + Pf1N1B4_875 0.78 -1.4 1,001,372 + +0.9 1,001,373 - Pf1N1B4_875 0.78 -0.7 1,001,373 - -1.4 1,001,530 - +0.6 1,001,612 - -0.1 1,001,762 - Pf1N1B4_876 0.16 -0.4 1,001,863 - Pf1N1B4_876 0.24 -0.4 1,001,932 + Pf1N1B4_876 0.30 +0.5 1,002,216 - Pf1N1B4_876 0.53 -0.9 1,002,298 + Pf1N1B4_876 0.59 +0.0 1,002,299 - +0.3 1,002,299 - Pf1N1B4_876 0.59 -1.0 1,002,299 - Pf1N1B4_876 0.59 -0.4 1,002,354 + Pf1N1B4_876 0.64 -0.1 1,002,355 - Pf1N1B4_876 0.64 -2.7 1,002,431 - Pf1N1B4_876 0.70 -1.0 1,002,809 + +0.0 1,002,810 - +0.1 1,002,810 - -1.3 1,002,814 - -0.6 1,002,814 - +0.1 1,002,814 - -0.9 1,002,829 - +0.1 1,002,829 - -1.1 1,002,848 + +1.9 1,002,849 - +1.9 1,002,849 - -0.3 1,002,849 - +0.9 1,002,849 - +0.7 1,002,849 - +0.9 1,002,849 - +0.2 1,002,892 - +0.5 1,002,892 - -0.0 1,002,957 + +0.1 1,002,975 + +0.6 1,002,976 - +1.3 1,003,007 + -0.0 1,003,007 + +0.1 1,003,010 + +0.6 1,003,010 + -0.6 1,003,010 + +1.2 1,003,010 + +0.9 1,003,010 + -0.9 1,003,010 + -0.1 1,003,010 + -1.1 1,003,010 + +0.2 1,003,011 - -0.2 1,003,011 - +0.9 1,003,011 - -0.1 1,003,011 - +0.7 1,003,011 - -0.7 1,003,011 - -0.4 1,003,154 + Pf1N1B4_877 0.13 +0.0 1,003,154 + Pf1N1B4_877 0.13 +1.5 1,003,154 + Pf1N1B4_877 0.13 +0.3 1,003,163 + Pf1N1B4_877 0.14 +2.0 1,003,163 + Pf1N1B4_877 0.14 +0.2 1,003,163 + -0.1 1,003,164 - Pf1N1B4_877 0.14 -0.1 1,003,164 - Pf1N1B4_877 0.14 +0.8 1,003,164 - Pf1N1B4_877 0.14 +0.1 1,003,169 + Pf1N1B4_877 0.14 +0.1 1,003,169 + Pf1N1B4_877 0.14 +1.7 1,003,169 + Pf1N1B4_877 0.14 +0.9 1,003,170 - Pf1N1B4_877 0.15 +0.9 1,003,170 - Pf1N1B4_877 0.15 +0.9 1,003,397 + Pf1N1B4_877 0.42 -0.9 1,003,398 - Pf1N1B4_877 0.43 +0.6 1,003,688 + Pf1N1B4_877 0.78 +0.3 1,003,688 + Pf1N1B4_877 0.78 +0.1 1,003,688 + Pf1N1B4_877 0.78 +0.4 1,003,688 + Pf1N1B4_877 0.78 +1.6 1,003,688 + Pf1N1B4_877 0.78 +1.6 1,003,688 + Pf1N1B4_877 0.78 +1.7 1,003,688 + Pf1N1B4_877 0.78 +0.6 1,003,688 + -1.1 1,003,688 + +1.9 1,003,688 + Pf1N1B4_877 0.78 -0.9 1,003,689 - Pf1N1B4_877 0.78 +0.3 1,003,689 - Pf1N1B4_877 0.78 +0.1 1,003,689 - Pf1N1B4_877 0.78 -0.1 1,003,689 - Pf1N1B4_877 0.78 -0.4 1,003,689 - Pf1N1B4_877 0.78 -1.3
Or see this region's nucleotide sequence