Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_854 and Pf1N1B4_855 are separated by 3 nucleotides Pf1N1B4_855 and Pf1N1B4_856 overlap by 1 nucleotides Pf1N1B4_856 and Pf1N1B4_857 are separated by 12 nucleotides
Pf1N1B4_854 - 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase (EC 1.17.7.1), at 983,667 to 984,776
_854
Pf1N1B4_855 - FIG021952: putative membrane protein, at 984,780 to 985,820
_855
Pf1N1B4_856 - Type IV pilus biogenesis protein PilF, at 985,820 to 986,578
_856
Pf1N1B4_857 - Ribosomal RNA large subunit methyltransferase N (EC 2.1.1.-), at 986,591 to 987,739
_857
Position (kb) 984
985
986 Strain fitness (log2 ratio) -2
-1
0
1
2 at 984.851 kb on - strand at 984.851 kb on - strand at 984.851 kb on - strand at 984.851 kb on - strand at 984.851 kb on - strand at 984.851 kb on - strand at 984.851 kb on - strand at 984.851 kb on - strand at 984.851 kb on - strand at 984.851 kb on - strand at 984.851 kb on - strand at 984.851 kb on - strand at 984.851 kb on - strand at 984.930 kb on - strand, within Pf1N1B4_855 at 984.930 kb on - strand, within Pf1N1B4_855 at 984.930 kb on - strand, within Pf1N1B4_855 at 984.930 kb on - strand, within Pf1N1B4_855 at 985.485 kb on - strand, within Pf1N1B4_855 at 985.837 kb on - strand at 985.846 kb on - strand at 985.846 kb on - strand at 985.944 kb on - strand, within Pf1N1B4_856 at 985.944 kb on - strand, within Pf1N1B4_856 at 985.944 kb on - strand at 985.949 kb on - strand, within Pf1N1B4_856 at 985.987 kb on - strand, within Pf1N1B4_856 at 985.990 kb on - strand, within Pf1N1B4_856 at 985.990 kb on - strand, within Pf1N1B4_856 at 986.007 kb on + strand, within Pf1N1B4_856 at 986.008 kb on - strand, within Pf1N1B4_856 at 986.008 kb on - strand, within Pf1N1B4_856 at 986.008 kb on - strand, within Pf1N1B4_856 at 986.008 kb on - strand, within Pf1N1B4_856 at 986.008 kb on - strand, within Pf1N1B4_856 at 986.008 kb on - strand, within Pf1N1B4_856 at 986.008 kb on - strand, within Pf1N1B4_856 at 986.008 kb on - strand, within Pf1N1B4_856 at 986.008 kb on - strand, within Pf1N1B4_856 at 986.023 kb on - strand, within Pf1N1B4_856 at 986.023 kb on - strand, within Pf1N1B4_856 at 986.439 kb on + strand, within Pf1N1B4_856 at 986.461 kb on - strand, within Pf1N1B4_856 at 986.578 kb on + strand at 986.578 kb on + strand at 986.578 kb on + strand at 986.579 kb on - strand at 986.579 kb on - strand at 986.579 kb on - strand at 986.579 kb on - strand at 986.579 kb on - strand at 986.592 kb on + strand at 986.592 kb on + strand at 986.592 kb on + strand at 986.592 kb on + strand at 986.592 kb on + strand at 986.593 kb on - strand at 986.593 kb on - strand at 986.593 kb on - strand at 986.596 kb on + strand at 986.596 kb on + strand at 986.597 kb on - strand at 986.597 kb on - strand at 986.597 kb on - strand at 986.656 kb on - strand at 986.656 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 984,851 - +2.1 984,851 - -1.7 984,851 - -0.0 984,851 - +0.3 984,851 - -1.4 984,851 - +0.9 984,851 - -0.4 984,851 - +0.6 984,851 - -1.9 984,851 - +0.6 984,851 - +0.5 984,851 - -0.4 984,851 - +1.4 984,930 - Pf1N1B4_855 0.14 +0.2 984,930 - Pf1N1B4_855 0.14 -0.6 984,930 - Pf1N1B4_855 0.14 -2.4 984,930 - Pf1N1B4_855 0.14 -0.6 985,485 - Pf1N1B4_855 0.68 -1.4 985,837 - -0.6 985,846 - +0.4 985,846 - +0.5 985,944 - Pf1N1B4_856 0.16 -0.1 985,944 - Pf1N1B4_856 0.16 +1.2 985,944 - +0.9 985,949 - Pf1N1B4_856 0.17 -0.1 985,987 - Pf1N1B4_856 0.22 +0.1 985,990 - Pf1N1B4_856 0.22 +0.5 985,990 - Pf1N1B4_856 0.22 -0.8 986,007 + Pf1N1B4_856 0.25 +2.5 986,008 - Pf1N1B4_856 0.25 +0.3 986,008 - Pf1N1B4_856 0.25 -2.4 986,008 - Pf1N1B4_856 0.25 -0.8 986,008 - Pf1N1B4_856 0.25 +0.1 986,008 - Pf1N1B4_856 0.25 -1.4 986,008 - Pf1N1B4_856 0.25 -0.2 986,008 - Pf1N1B4_856 0.25 +0.4 986,008 - Pf1N1B4_856 0.25 +1.2 986,008 - Pf1N1B4_856 0.25 -0.9 986,023 - Pf1N1B4_856 0.27 -1.1 986,023 - Pf1N1B4_856 0.27 +1.0 986,439 + Pf1N1B4_856 0.82 +1.1 986,461 - Pf1N1B4_856 0.84 +0.2 986,578 + +0.9 986,578 + +2.7 986,578 + +0.1 986,579 - -0.1 986,579 - +1.9 986,579 - -0.6 986,579 - +1.1 986,579 - -0.3 986,592 + +1.3 986,592 + -0.3 986,592 + -0.3 986,592 + +1.3 986,592 + -1.7 986,593 - +0.7 986,593 - +0.3 986,593 - +0.4 986,596 + +0.0 986,596 + +1.5 986,597 - -1.4 986,597 - +1.0 986,597 - +0.1 986,656 - +0.1 986,656 - +0.3
Or see this region's nucleotide sequence