Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_794 and Pf1N1B4_795 are separated by 406 nucleotides Pf1N1B4_795 and Pf1N1B4_796 are separated by 8 nucleotides
Pf1N1B4_794 - Signal transduction histidine kinase, at 906,483 to 907,886
_794
Pf1N1B4_795 - hypothetical protein, at 908,293 to 908,577
_795
Pf1N1B4_796 - putative membrane protein, at 908,586 to 910,139
_796
Position (kb) 908
909 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 907.494 kb on + strand, within Pf1N1B4_794 at 907.494 kb on + strand, within Pf1N1B4_794 at 907.494 kb on + strand, within Pf1N1B4_794 at 907.495 kb on - strand, within Pf1N1B4_794 at 907.684 kb on + strand, within Pf1N1B4_794 at 907.684 kb on + strand, within Pf1N1B4_794 at 907.684 kb on + strand, within Pf1N1B4_794 at 907.684 kb on + strand, within Pf1N1B4_794 at 907.684 kb on + strand, within Pf1N1B4_794 at 907.684 kb on + strand at 907.684 kb on + strand, within Pf1N1B4_794 at 907.684 kb on + strand, within Pf1N1B4_794 at 907.684 kb on + strand, within Pf1N1B4_794 at 907.684 kb on + strand, within Pf1N1B4_794 at 907.684 kb on + strand, within Pf1N1B4_794 at 907.685 kb on - strand, within Pf1N1B4_794 at 907.685 kb on - strand, within Pf1N1B4_794 at 907.685 kb on - strand, within Pf1N1B4_794 at 907.685 kb on - strand, within Pf1N1B4_794 at 907.685 kb on - strand, within Pf1N1B4_794 at 907.685 kb on - strand, within Pf1N1B4_794 at 907.685 kb on - strand, within Pf1N1B4_794 at 907.685 kb on - strand, within Pf1N1B4_794 at 907.685 kb on - strand, within Pf1N1B4_794 at 907.685 kb on - strand at 907.689 kb on - strand, within Pf1N1B4_794 at 907.728 kb on + strand, within Pf1N1B4_794 at 907.729 kb on - strand, within Pf1N1B4_794 at 907.729 kb on - strand, within Pf1N1B4_794 at 907.779 kb on + strand at 907.779 kb on + strand at 907.780 kb on - strand at 907.780 kb on - strand at 907.808 kb on + strand at 907.809 kb on - strand at 907.884 kb on + strand at 907.884 kb on + strand at 907.885 kb on - strand at 907.885 kb on - strand at 907.914 kb on + strand at 907.915 kb on - strand at 907.915 kb on - strand at 908.099 kb on + strand at 908.207 kb on + strand at 908.208 kb on - strand at 908.237 kb on + strand at 908.237 kb on + strand at 908.237 kb on + strand at 908.237 kb on + strand at 908.237 kb on + strand at 908.238 kb on - strand at 908.238 kb on - strand at 908.238 kb on - strand at 908.238 kb on - strand at 908.238 kb on - strand at 908.238 kb on - strand at 908.238 kb on - strand at 908.238 kb on - strand at 908.238 kb on - strand at 908.238 kb on - strand at 908.238 kb on - strand at 908.238 kb on - strand at 908.238 kb on - strand at 908.238 kb on - strand at 908.264 kb on + strand at 908.264 kb on + strand at 908.264 kb on + strand at 908.265 kb on - strand at 908.265 kb on - strand at 908.265 kb on - strand at 908.265 kb on - strand at 908.265 kb on - strand at 908.265 kb on - strand at 908.265 kb on - strand at 908.265 kb on - strand at 908.269 kb on - strand at 908.304 kb on - strand at 908.332 kb on + strand, within Pf1N1B4_795 at 908.332 kb on + strand, within Pf1N1B4_795 at 908.333 kb on - strand, within Pf1N1B4_795 at 908.405 kb on - strand at 908.442 kb on + strand, within Pf1N1B4_795 at 908.442 kb on + strand, within Pf1N1B4_795 at 908.443 kb on - strand, within Pf1N1B4_795 at 908.443 kb on - strand, within Pf1N1B4_795 at 908.443 kb on - strand at 908.443 kb on - strand, within Pf1N1B4_795 at 908.443 kb on - strand, within Pf1N1B4_795 at 908.550 kb on + strand at 908.550 kb on + strand at 908.550 kb on + strand at 908.551 kb on - strand at 908.551 kb on - strand at 908.551 kb on - strand at 908.551 kb on - strand at 908.551 kb on - strand at 908.851 kb on + strand, within Pf1N1B4_796 at 908.851 kb on + strand, within Pf1N1B4_796 at 908.851 kb on + strand, within Pf1N1B4_796 at 908.851 kb on + strand, within Pf1N1B4_796 at 908.853 kb on + strand, within Pf1N1B4_796 at 908.854 kb on - strand, within Pf1N1B4_796 at 908.881 kb on + strand, within Pf1N1B4_796 at 908.881 kb on - strand, within Pf1N1B4_796 at 908.882 kb on - strand, within Pf1N1B4_796 at 908.882 kb on - strand, within Pf1N1B4_796 at 908.884 kb on + strand, within Pf1N1B4_796 at 908.884 kb on + strand, within Pf1N1B4_796 at 908.885 kb on - strand, within Pf1N1B4_796 at 908.885 kb on - strand, within Pf1N1B4_796 at 908.888 kb on + strand at 908.888 kb on + strand, within Pf1N1B4_796 at 908.888 kb on + strand, within Pf1N1B4_796 at 908.888 kb on + strand, within Pf1N1B4_796 at 908.889 kb on - strand, within Pf1N1B4_796 at 908.899 kb on + strand, within Pf1N1B4_796 at 908.899 kb on + strand, within Pf1N1B4_796 at 908.899 kb on + strand at 908.905 kb on + strand, within Pf1N1B4_796 at 908.940 kb on + strand, within Pf1N1B4_796 at 908.940 kb on + strand, within Pf1N1B4_796 at 908.940 kb on + strand, within Pf1N1B4_796 at 909.045 kb on + strand, within Pf1N1B4_796 at 909.045 kb on + strand, within Pf1N1B4_796 at 909.045 kb on + strand, within Pf1N1B4_796 at 909.045 kb on + strand, within Pf1N1B4_796 at 909.046 kb on - strand at 909.470 kb on - strand, within Pf1N1B4_796
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 907,494 + Pf1N1B4_794 0.72 +1.0 907,494 + Pf1N1B4_794 0.72 -1.0 907,494 + Pf1N1B4_794 0.72 +0.3 907,495 - Pf1N1B4_794 0.72 +1.2 907,684 + Pf1N1B4_794 0.86 +0.6 907,684 + Pf1N1B4_794 0.86 +0.9 907,684 + Pf1N1B4_794 0.86 -1.5 907,684 + Pf1N1B4_794 0.86 +0.9 907,684 + Pf1N1B4_794 0.86 -0.8 907,684 + -0.1 907,684 + Pf1N1B4_794 0.86 -0.6 907,684 + Pf1N1B4_794 0.86 +0.2 907,684 + Pf1N1B4_794 0.86 -1.3 907,684 + Pf1N1B4_794 0.86 +0.3 907,684 + Pf1N1B4_794 0.86 +0.4 907,685 - Pf1N1B4_794 0.86 +0.1 907,685 - Pf1N1B4_794 0.86 +0.6 907,685 - Pf1N1B4_794 0.86 +0.0 907,685 - Pf1N1B4_794 0.86 -1.2 907,685 - Pf1N1B4_794 0.86 +0.2 907,685 - Pf1N1B4_794 0.86 -2.0 907,685 - Pf1N1B4_794 0.86 +0.3 907,685 - Pf1N1B4_794 0.86 -1.1 907,685 - Pf1N1B4_794 0.86 -1.0 907,685 - +0.9 907,689 - Pf1N1B4_794 0.86 -0.4 907,728 + Pf1N1B4_794 0.89 +0.7 907,729 - Pf1N1B4_794 0.89 -1.0 907,729 - Pf1N1B4_794 0.89 +0.9 907,779 + +0.8 907,779 + +0.9 907,780 - -0.3 907,780 - +0.9 907,808 + -1.5 907,809 - -0.1 907,884 + +0.5 907,884 + -0.2 907,885 - -2.2 907,885 - +0.5 907,914 + -1.0 907,915 - +0.6 907,915 - +0.2 908,099 + +0.4 908,207 + +0.4 908,208 - +0.3 908,237 + -0.0 908,237 + +0.3 908,237 + +0.0 908,237 + +0.1 908,237 + +1.6 908,238 - +0.3 908,238 - -2.1 908,238 - +1.8 908,238 - +1.0 908,238 - -1.7 908,238 - +1.0 908,238 - +0.4 908,238 - -0.7 908,238 - +0.8 908,238 - +0.6 908,238 - +0.0 908,238 - +0.8 908,238 - +0.3 908,238 - +0.5 908,264 + +0.2 908,264 + -0.5 908,264 + -0.2 908,265 - -1.2 908,265 - -0.7 908,265 - -0.2 908,265 - +1.0 908,265 - +0.8 908,265 - -0.0 908,265 - -0.8 908,265 - +1.1 908,269 - -1.6 908,304 - +1.0 908,332 + Pf1N1B4_795 0.14 +0.2 908,332 + Pf1N1B4_795 0.14 -2.9 908,333 - Pf1N1B4_795 0.14 -0.2 908,405 - +0.1 908,442 + Pf1N1B4_795 0.52 -2.3 908,442 + Pf1N1B4_795 0.52 +0.7 908,443 - Pf1N1B4_795 0.53 -1.0 908,443 - Pf1N1B4_795 0.53 +1.0 908,443 - +0.8 908,443 - Pf1N1B4_795 0.53 -1.7 908,443 - Pf1N1B4_795 0.53 -1.7 908,550 + -2.0 908,550 + -0.4 908,550 + +1.4 908,551 - -0.6 908,551 - +0.3 908,551 - +0.3 908,551 - -1.0 908,551 - +0.4 908,851 + Pf1N1B4_796 0.17 +1.3 908,851 + Pf1N1B4_796 0.17 -1.2 908,851 + Pf1N1B4_796 0.17 -1.2 908,851 + Pf1N1B4_796 0.17 +2.4 908,853 + Pf1N1B4_796 0.17 +0.3 908,854 - Pf1N1B4_796 0.17 +2.8 908,881 + Pf1N1B4_796 0.19 +0.3 908,881 - Pf1N1B4_796 0.19 -0.6 908,882 - Pf1N1B4_796 0.19 -0.2 908,882 - Pf1N1B4_796 0.19 -0.2 908,884 + Pf1N1B4_796 0.19 -0.2 908,884 + Pf1N1B4_796 0.19 +2.2 908,885 - Pf1N1B4_796 0.19 -0.9 908,885 - Pf1N1B4_796 0.19 +0.3 908,888 + -0.5 908,888 + Pf1N1B4_796 0.19 +0.8 908,888 + Pf1N1B4_796 0.19 +1.7 908,888 + Pf1N1B4_796 0.19 +0.3 908,889 - Pf1N1B4_796 0.19 +0.5 908,899 + Pf1N1B4_796 0.20 +0.9 908,899 + Pf1N1B4_796 0.20 +1.0 908,899 + +0.9 908,905 + Pf1N1B4_796 0.21 -0.5 908,940 + Pf1N1B4_796 0.23 -0.7 908,940 + Pf1N1B4_796 0.23 +0.0 908,940 + Pf1N1B4_796 0.23 +0.3 909,045 + Pf1N1B4_796 0.30 -0.1 909,045 + Pf1N1B4_796 0.30 +0.2 909,045 + Pf1N1B4_796 0.30 +0.6 909,045 + Pf1N1B4_796 0.30 +0.3 909,046 - +0.9 909,470 - Pf1N1B4_796 0.57 -0.2
Or see this region's nucleotide sequence