Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_790 and Pf1N1B4_791 are separated by 46 nucleotides Pf1N1B4_791 and Pf1N1B4_792 overlap by 37 nucleotides Pf1N1B4_792 and Pf1N1B4_793 overlap by 4 nucleotides
Pf1N1B4_790 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 901,799 to 903,673
_790
Pf1N1B4_791 - Cytochrome c551 peroxidase (EC 1.11.1.5), at 903,720 to 904,133
_791
Pf1N1B4_792 - Cytochrome c551 peroxidase (EC 1.11.1.5), at 904,097 to 904,678
_792
Pf1N1B4_793 - sensor histidine kinase, at 904,675 to 906,486
_793
Position (kb)
904
905 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 903.222 kb on + strand at 903.222 kb on + strand, within Pf1N1B4_790 at 903.222 kb on + strand, within Pf1N1B4_790 at 903.223 kb on - strand, within Pf1N1B4_790 at 903.223 kb on - strand, within Pf1N1B4_790 at 903.279 kb on + strand, within Pf1N1B4_790 at 903.279 kb on + strand, within Pf1N1B4_790 at 903.279 kb on + strand, within Pf1N1B4_790 at 903.386 kb on + strand, within Pf1N1B4_790 at 903.591 kb on - strand at 903.591 kb on - strand at 903.593 kb on + strand at 903.593 kb on + strand at 903.594 kb on - strand at 903.594 kb on - strand at 903.594 kb on - strand at 903.594 kb on - strand at 903.594 kb on - strand at 903.632 kb on + strand at 903.633 kb on - strand at 903.633 kb on - strand at 903.716 kb on + strand at 903.716 kb on + strand at 903.716 kb on + strand at 903.716 kb on + strand at 903.717 kb on - strand at 903.717 kb on - strand at 903.717 kb on - strand at 903.717 kb on - strand at 903.735 kb on + strand at 903.736 kb on - strand at 903.736 kb on - strand at 903.736 kb on - strand at 903.736 kb on - strand at 903.738 kb on + strand at 903.893 kb on + strand, within Pf1N1B4_791 at 903.894 kb on - strand, within Pf1N1B4_791 at 904.148 kb on + strand at 904.172 kb on + strand, within Pf1N1B4_792 at 904.220 kb on + strand, within Pf1N1B4_792 at 904.221 kb on - strand, within Pf1N1B4_792 at 904.221 kb on - strand, within Pf1N1B4_792 at 904.221 kb on - strand, within Pf1N1B4_792 at 904.221 kb on - strand, within Pf1N1B4_792 at 904.221 kb on - strand, within Pf1N1B4_792 at 904.262 kb on + strand, within Pf1N1B4_792 at 904.263 kb on - strand, within Pf1N1B4_792 at 904.304 kb on + strand, within Pf1N1B4_792 at 904.304 kb on + strand, within Pf1N1B4_792 at 904.304 kb on + strand, within Pf1N1B4_792 at 904.304 kb on + strand, within Pf1N1B4_792 at 904.305 kb on - strand, within Pf1N1B4_792 at 904.305 kb on - strand, within Pf1N1B4_792 at 904.328 kb on + strand, within Pf1N1B4_792 at 904.329 kb on - strand, within Pf1N1B4_792 at 904.404 kb on - strand, within Pf1N1B4_792 at 904.404 kb on - strand, within Pf1N1B4_792 at 904.449 kb on - strand, within Pf1N1B4_792 at 904.449 kb on - strand, within Pf1N1B4_792 at 904.526 kb on + strand, within Pf1N1B4_792 at 904.555 kb on - strand, within Pf1N1B4_792 at 904.684 kb on + strand at 904.786 kb on + strand at 904.882 kb on + strand, within Pf1N1B4_793 at 904.883 kb on - strand, within Pf1N1B4_793 at 904.883 kb on - strand, within Pf1N1B4_793 at 904.883 kb on - strand, within Pf1N1B4_793 at 904.883 kb on - strand, within Pf1N1B4_793 at 904.999 kb on + strand, within Pf1N1B4_793 at 905.150 kb on + strand at 905.151 kb on - strand, within Pf1N1B4_793 at 905.173 kb on + strand, within Pf1N1B4_793 at 905.173 kb on + strand, within Pf1N1B4_793 at 905.173 kb on + strand, within Pf1N1B4_793 at 905.174 kb on - strand at 905.174 kb on - strand, within Pf1N1B4_793 at 905.174 kb on - strand, within Pf1N1B4_793 at 905.174 kb on - strand, within Pf1N1B4_793 at 905.174 kb on - strand, within Pf1N1B4_793 at 905.412 kb on + strand, within Pf1N1B4_793 at 905.412 kb on + strand, within Pf1N1B4_793 at 905.413 kb on - strand, within Pf1N1B4_793 at 905.413 kb on - strand, within Pf1N1B4_793 at 905.413 kb on - strand, within Pf1N1B4_793 at 905.473 kb on + strand, within Pf1N1B4_793 at 905.473 kb on + strand, within Pf1N1B4_793 at 905.473 kb on + strand, within Pf1N1B4_793 at 905.473 kb on + strand, within Pf1N1B4_793 at 905.473 kb on + strand, within Pf1N1B4_793 at 905.474 kb on - strand, within Pf1N1B4_793 at 905.474 kb on - strand at 905.474 kb on - strand, within Pf1N1B4_793 at 905.474 kb on - strand, within Pf1N1B4_793 at 905.474 kb on - strand, within Pf1N1B4_793 at 905.474 kb on - strand, within Pf1N1B4_793 at 905.474 kb on - strand, within Pf1N1B4_793 at 905.474 kb on - strand, within Pf1N1B4_793 at 905.483 kb on + strand, within Pf1N1B4_793 at 905.515 kb on + strand, within Pf1N1B4_793 at 905.515 kb on + strand, within Pf1N1B4_793 at 905.515 kb on + strand, within Pf1N1B4_793 at 905.516 kb on - strand, within Pf1N1B4_793 at 905.516 kb on - strand, within Pf1N1B4_793 at 905.516 kb on - strand, within Pf1N1B4_793 at 905.516 kb on - strand, within Pf1N1B4_793
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 903,222 + -0.7 903,222 + Pf1N1B4_790 0.76 +0.0 903,222 + Pf1N1B4_790 0.76 -0.7 903,223 - Pf1N1B4_790 0.76 +0.1 903,223 - Pf1N1B4_790 0.76 -0.6 903,279 + Pf1N1B4_790 0.79 -0.4 903,279 + Pf1N1B4_790 0.79 -2.3 903,279 + Pf1N1B4_790 0.79 +0.0 903,386 + Pf1N1B4_790 0.85 -0.0 903,591 - +1.1 903,591 - +0.2 903,593 + +1.9 903,593 + -1.1 903,594 - +2.9 903,594 - -1.3 903,594 - +0.8 903,594 - +1.0 903,594 - -2.0 903,632 + -0.6 903,633 - +0.4 903,633 - +0.7 903,716 + -0.1 903,716 + +1.9 903,716 + -0.5 903,716 + -0.7 903,717 - +1.1 903,717 - +0.0 903,717 - +1.3 903,717 - -2.5 903,735 + -0.8 903,736 - -0.1 903,736 - -0.4 903,736 - +0.4 903,736 - +1.4 903,738 + -0.5 903,893 + Pf1N1B4_791 0.42 -1.7 903,894 - Pf1N1B4_791 0.42 -0.1 904,148 + -0.7 904,172 + Pf1N1B4_792 0.13 +0.4 904,220 + Pf1N1B4_792 0.21 -0.1 904,221 - Pf1N1B4_792 0.21 +0.3 904,221 - Pf1N1B4_792 0.21 +1.5 904,221 - Pf1N1B4_792 0.21 +0.2 904,221 - Pf1N1B4_792 0.21 -0.1 904,221 - Pf1N1B4_792 0.21 +1.4 904,262 + Pf1N1B4_792 0.28 +0.9 904,263 - Pf1N1B4_792 0.29 -2.0 904,304 + Pf1N1B4_792 0.36 -0.7 904,304 + Pf1N1B4_792 0.36 +0.9 904,304 + Pf1N1B4_792 0.36 -0.3 904,304 + Pf1N1B4_792 0.36 +0.9 904,305 - Pf1N1B4_792 0.36 -1.7 904,305 - Pf1N1B4_792 0.36 +0.9 904,328 + Pf1N1B4_792 0.40 +0.4 904,329 - Pf1N1B4_792 0.40 -0.3 904,404 - Pf1N1B4_792 0.53 +1.4 904,404 - Pf1N1B4_792 0.53 +0.2 904,449 - Pf1N1B4_792 0.60 +0.6 904,449 - Pf1N1B4_792 0.60 +0.3 904,526 + Pf1N1B4_792 0.74 +0.4 904,555 - Pf1N1B4_792 0.79 +1.3 904,684 + -0.4 904,786 + +1.3 904,882 + Pf1N1B4_793 0.11 +0.9 904,883 - Pf1N1B4_793 0.11 -0.6 904,883 - Pf1N1B4_793 0.11 +0.7 904,883 - Pf1N1B4_793 0.11 -0.3 904,883 - Pf1N1B4_793 0.11 -0.6 904,999 + Pf1N1B4_793 0.18 -0.0 905,150 + +0.9 905,151 - Pf1N1B4_793 0.26 +2.4 905,173 + Pf1N1B4_793 0.27 +0.3 905,173 + Pf1N1B4_793 0.27 -0.9 905,173 + Pf1N1B4_793 0.27 +0.2 905,174 - -0.7 905,174 - Pf1N1B4_793 0.28 -0.1 905,174 - Pf1N1B4_793 0.28 -2.3 905,174 - Pf1N1B4_793 0.28 +1.7 905,174 - Pf1N1B4_793 0.28 -0.5 905,412 + Pf1N1B4_793 0.41 -1.0 905,412 + Pf1N1B4_793 0.41 +0.5 905,413 - Pf1N1B4_793 0.41 +0.3 905,413 - Pf1N1B4_793 0.41 -0.3 905,413 - Pf1N1B4_793 0.41 +1.5 905,473 + Pf1N1B4_793 0.44 +0.9 905,473 + Pf1N1B4_793 0.44 +1.2 905,473 + Pf1N1B4_793 0.44 +0.1 905,473 + Pf1N1B4_793 0.44 +0.6 905,473 + Pf1N1B4_793 0.44 -2.0 905,474 - Pf1N1B4_793 0.44 -1.5 905,474 - +1.9 905,474 - Pf1N1B4_793 0.44 -0.0 905,474 - Pf1N1B4_793 0.44 +0.4 905,474 - Pf1N1B4_793 0.44 +3.0 905,474 - Pf1N1B4_793 0.44 +0.2 905,474 - Pf1N1B4_793 0.44 +0.4 905,474 - Pf1N1B4_793 0.44 -1.5 905,483 + Pf1N1B4_793 0.45 +0.9 905,515 + Pf1N1B4_793 0.46 -1.5 905,515 + Pf1N1B4_793 0.46 +1.1 905,515 + Pf1N1B4_793 0.46 -2.0 905,516 - Pf1N1B4_793 0.46 -1.3 905,516 - Pf1N1B4_793 0.46 +0.9 905,516 - Pf1N1B4_793 0.46 +0.3 905,516 - Pf1N1B4_793 0.46 +0.9
Or see this region's nucleotide sequence