Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_755 and Pf1N1B4_756 are separated by 175 nucleotides Pf1N1B4_756 and Pf1N1B4_757 are separated by 274 nucleotides
Pf1N1B4_755 - RecA-family ATPase, at 858,442 to 859,923
_755
Pf1N1B4_756 - hypothetical protein, at 860,099 to 860,287
_756
Pf1N1B4_757 - phage-related integrase, at 860,562 to 861,812
_757
Position (kb)
860
861 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 859.237 kb on + strand, within Pf1N1B4_755 at 859.237 kb on + strand, within Pf1N1B4_755 at 859.392 kb on + strand, within Pf1N1B4_755 at 859.392 kb on + strand, within Pf1N1B4_755 at 859.392 kb on + strand at 859.392 kb on + strand, within Pf1N1B4_755 at 859.392 kb on + strand, within Pf1N1B4_755 at 859.392 kb on + strand, within Pf1N1B4_755 at 859.392 kb on + strand, within Pf1N1B4_755 at 859.393 kb on - strand, within Pf1N1B4_755 at 859.393 kb on - strand, within Pf1N1B4_755 at 859.393 kb on - strand, within Pf1N1B4_755 at 859.393 kb on - strand, within Pf1N1B4_755 at 859.393 kb on - strand, within Pf1N1B4_755 at 859.393 kb on - strand, within Pf1N1B4_755 at 859.396 kb on + strand, within Pf1N1B4_755 at 859.396 kb on + strand, within Pf1N1B4_755 at 859.396 kb on + strand, within Pf1N1B4_755 at 859.396 kb on + strand, within Pf1N1B4_755 at 859.397 kb on - strand, within Pf1N1B4_755 at 859.397 kb on - strand, within Pf1N1B4_755 at 859.397 kb on - strand, within Pf1N1B4_755 at 859.397 kb on - strand, within Pf1N1B4_755 at 859.450 kb on + strand, within Pf1N1B4_755 at 859.450 kb on + strand, within Pf1N1B4_755 at 859.450 kb on + strand, within Pf1N1B4_755 at 859.450 kb on + strand, within Pf1N1B4_755 at 859.451 kb on - strand, within Pf1N1B4_755 at 859.451 kb on - strand, within Pf1N1B4_755 at 859.451 kb on - strand, within Pf1N1B4_755 at 859.634 kb on + strand, within Pf1N1B4_755 at 859.634 kb on + strand, within Pf1N1B4_755 at 859.634 kb on + strand, within Pf1N1B4_755 at 859.635 kb on - strand, within Pf1N1B4_755 at 859.635 kb on - strand, within Pf1N1B4_755 at 859.635 kb on - strand, within Pf1N1B4_755 at 859.635 kb on - strand, within Pf1N1B4_755 at 859.635 kb on - strand at 859.635 kb on - strand, within Pf1N1B4_755 at 859.658 kb on + strand, within Pf1N1B4_755 at 859.658 kb on + strand at 859.658 kb on + strand, within Pf1N1B4_755 at 859.658 kb on + strand, within Pf1N1B4_755 at 859.658 kb on + strand, within Pf1N1B4_755 at 859.658 kb on + strand, within Pf1N1B4_755 at 859.658 kb on + strand, within Pf1N1B4_755 at 859.658 kb on + strand, within Pf1N1B4_755 at 859.658 kb on + strand, within Pf1N1B4_755 at 859.659 kb on + strand at 859.659 kb on - strand, within Pf1N1B4_755 at 859.659 kb on - strand, within Pf1N1B4_755 at 859.659 kb on - strand, within Pf1N1B4_755 at 859.659 kb on - strand, within Pf1N1B4_755 at 859.659 kb on - strand, within Pf1N1B4_755 at 859.659 kb on - strand, within Pf1N1B4_755 at 859.662 kb on + strand, within Pf1N1B4_755 at 859.673 kb on + strand at 859.673 kb on + strand, within Pf1N1B4_755 at 859.673 kb on + strand, within Pf1N1B4_755 at 859.673 kb on + strand, within Pf1N1B4_755 at 859.673 kb on + strand, within Pf1N1B4_755 at 859.674 kb on - strand, within Pf1N1B4_755 at 859.674 kb on - strand, within Pf1N1B4_755 at 859.674 kb on - strand, within Pf1N1B4_755 at 859.674 kb on - strand, within Pf1N1B4_755 at 859.674 kb on - strand at 859.674 kb on - strand, within Pf1N1B4_755 at 859.687 kb on + strand, within Pf1N1B4_755 at 859.739 kb on + strand, within Pf1N1B4_755 at 859.745 kb on + strand, within Pf1N1B4_755 at 859.745 kb on + strand, within Pf1N1B4_755 at 859.745 kb on + strand, within Pf1N1B4_755 at 859.745 kb on + strand, within Pf1N1B4_755 at 859.746 kb on - strand, within Pf1N1B4_755 at 859.746 kb on - strand, within Pf1N1B4_755 at 859.746 kb on - strand, within Pf1N1B4_755 at 859.814 kb on + strand at 859.815 kb on - strand at 859.815 kb on - strand at 859.903 kb on + strand at 859.903 kb on + strand at 859.904 kb on - strand at 859.904 kb on - strand at 859.923 kb on + strand at 859.923 kb on + strand at 859.923 kb on + strand at 859.923 kb on + strand at 859.923 kb on + strand at 859.923 kb on + strand at 859.923 kb on + strand at 859.923 kb on + strand at 859.925 kb on + strand at 859.925 kb on + strand at 859.925 kb on + strand at 859.925 kb on + strand at 859.925 kb on + strand at 859.925 kb on + strand at 859.925 kb on + strand at 859.925 kb on + strand at 859.926 kb on - strand at 859.926 kb on - strand at 859.926 kb on - strand at 859.926 kb on - strand at 859.959 kb on + strand at 859.960 kb on - strand at 859.961 kb on + strand at 859.962 kb on - strand at 859.963 kb on + strand at 859.963 kb on + strand at 859.963 kb on + strand at 859.963 kb on + strand at 859.963 kb on + strand at 859.964 kb on - strand at 859.964 kb on - strand at 859.964 kb on - strand at 859.964 kb on - strand at 860.053 kb on - strand at 860.053 kb on - strand at 860.100 kb on + strand at 860.101 kb on - strand at 860.101 kb on - strand at 860.101 kb on - strand at 860.101 kb on - strand at 860.180 kb on + strand, within Pf1N1B4_756 at 860.181 kb on - strand, within Pf1N1B4_756 at 860.181 kb on - strand, within Pf1N1B4_756 at 860.181 kb on - strand at 860.183 kb on - strand, within Pf1N1B4_756 at 860.185 kb on - strand, within Pf1N1B4_756 at 860.372 kb on + strand at 860.558 kb on - strand at 860.569 kb on - strand at 860.708 kb on + strand, within Pf1N1B4_757 at 860.709 kb on - strand, within Pf1N1B4_757 at 860.719 kb on + strand, within Pf1N1B4_757 at 860.720 kb on - strand, within Pf1N1B4_757 at 860.777 kb on + strand, within Pf1N1B4_757 at 860.784 kb on - strand, within Pf1N1B4_757 at 860.784 kb on - strand, within Pf1N1B4_757 at 860.835 kb on + strand, within Pf1N1B4_757 at 860.835 kb on + strand, within Pf1N1B4_757 at 860.835 kb on + strand, within Pf1N1B4_757 at 860.835 kb on + strand, within Pf1N1B4_757 at 860.835 kb on + strand, within Pf1N1B4_757 at 860.835 kb on + strand, within Pf1N1B4_757 at 860.835 kb on + strand, within Pf1N1B4_757 at 860.836 kb on - strand, within Pf1N1B4_757 at 860.836 kb on - strand at 860.836 kb on - strand, within Pf1N1B4_757 at 860.836 kb on - strand, within Pf1N1B4_757 at 860.976 kb on + strand at 860.976 kb on + strand, within Pf1N1B4_757 at 861.037 kb on - strand, within Pf1N1B4_757 at 861.037 kb on - strand, within Pf1N1B4_757 at 861.037 kb on - strand, within Pf1N1B4_757 at 861.037 kb on - strand, within Pf1N1B4_757 at 861.049 kb on + strand, within Pf1N1B4_757 at 861.049 kb on + strand, within Pf1N1B4_757 at 861.049 kb on + strand, within Pf1N1B4_757 at 861.050 kb on - strand, within Pf1N1B4_757 at 861.050 kb on - strand, within Pf1N1B4_757 at 861.050 kb on - strand, within Pf1N1B4_757 at 861.079 kb on - strand, within Pf1N1B4_757 at 861.188 kb on + strand, within Pf1N1B4_757 at 861.189 kb on - strand, within Pf1N1B4_757 at 861.284 kb on + strand, within Pf1N1B4_757 at 861.284 kb on + strand, within Pf1N1B4_757 at 861.285 kb on - strand, within Pf1N1B4_757 at 861.285 kb on - strand, within Pf1N1B4_757 at 861.285 kb on - strand, within Pf1N1B4_757 at 861.285 kb on - strand, within Pf1N1B4_757 at 861.285 kb on - strand, within Pf1N1B4_757 at 861.285 kb on - strand, within Pf1N1B4_757 at 861.285 kb on - strand, within Pf1N1B4_757
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 859,237 + Pf1N1B4_755 0.54 -0.2 859,237 + Pf1N1B4_755 0.54 -0.5 859,392 + Pf1N1B4_755 0.64 +0.8 859,392 + Pf1N1B4_755 0.64 +0.3 859,392 + +0.3 859,392 + Pf1N1B4_755 0.64 -1.0 859,392 + Pf1N1B4_755 0.64 -0.6 859,392 + Pf1N1B4_755 0.64 +1.2 859,392 + Pf1N1B4_755 0.64 -0.4 859,393 - Pf1N1B4_755 0.64 +0.7 859,393 - Pf1N1B4_755 0.64 +0.3 859,393 - Pf1N1B4_755 0.64 +0.5 859,393 - Pf1N1B4_755 0.64 -0.6 859,393 - Pf1N1B4_755 0.64 -0.9 859,393 - Pf1N1B4_755 0.64 -1.6 859,396 + Pf1N1B4_755 0.64 -0.7 859,396 + Pf1N1B4_755 0.64 -0.2 859,396 + Pf1N1B4_755 0.64 +0.6 859,396 + Pf1N1B4_755 0.64 -0.2 859,397 - Pf1N1B4_755 0.64 -0.7 859,397 - Pf1N1B4_755 0.64 -0.4 859,397 - Pf1N1B4_755 0.64 +0.4 859,397 - Pf1N1B4_755 0.64 +0.8 859,450 + Pf1N1B4_755 0.68 -0.2 859,450 + Pf1N1B4_755 0.68 -0.6 859,450 + Pf1N1B4_755 0.68 +0.1 859,450 + Pf1N1B4_755 0.68 -0.3 859,451 - Pf1N1B4_755 0.68 +0.4 859,451 - Pf1N1B4_755 0.68 +0.4 859,451 - Pf1N1B4_755 0.68 +1.6 859,634 + Pf1N1B4_755 0.80 -0.6 859,634 + Pf1N1B4_755 0.80 +0.8 859,634 + Pf1N1B4_755 0.80 +0.1 859,635 - Pf1N1B4_755 0.80 -0.1 859,635 - Pf1N1B4_755 0.80 +1.8 859,635 - Pf1N1B4_755 0.80 -0.4 859,635 - Pf1N1B4_755 0.80 +0.3 859,635 - -0.2 859,635 - Pf1N1B4_755 0.80 +0.8 859,658 + Pf1N1B4_755 0.82 -0.6 859,658 + -0.2 859,658 + Pf1N1B4_755 0.82 -1.6 859,658 + Pf1N1B4_755 0.82 -1.7 859,658 + Pf1N1B4_755 0.82 -1.7 859,658 + Pf1N1B4_755 0.82 -0.5 859,658 + Pf1N1B4_755 0.82 +0.6 859,658 + Pf1N1B4_755 0.82 +0.8 859,658 + Pf1N1B4_755 0.82 -0.1 859,659 + +2.8 859,659 - Pf1N1B4_755 0.82 -0.2 859,659 - Pf1N1B4_755 0.82 -1.5 859,659 - Pf1N1B4_755 0.82 -1.2 859,659 - Pf1N1B4_755 0.82 -0.4 859,659 - Pf1N1B4_755 0.82 +2.2 859,659 - Pf1N1B4_755 0.82 +2.1 859,662 + Pf1N1B4_755 0.82 -0.3 859,673 + +0.3 859,673 + Pf1N1B4_755 0.83 -1.3 859,673 + Pf1N1B4_755 0.83 +0.8 859,673 + Pf1N1B4_755 0.83 +0.1 859,673 + Pf1N1B4_755 0.83 -2.3 859,674 - Pf1N1B4_755 0.83 -0.5 859,674 - Pf1N1B4_755 0.83 -0.3 859,674 - Pf1N1B4_755 0.83 -1.5 859,674 - Pf1N1B4_755 0.83 +1.1 859,674 - -1.5 859,674 - Pf1N1B4_755 0.83 +0.5 859,687 + Pf1N1B4_755 0.84 +0.6 859,739 + Pf1N1B4_755 0.88 -0.7 859,745 + Pf1N1B4_755 0.88 -0.2 859,745 + Pf1N1B4_755 0.88 +0.1 859,745 + Pf1N1B4_755 0.88 -1.9 859,745 + Pf1N1B4_755 0.88 -1.2 859,746 - Pf1N1B4_755 0.88 -1.2 859,746 - Pf1N1B4_755 0.88 +0.2 859,746 - Pf1N1B4_755 0.88 -0.3 859,814 + +0.8 859,815 - +1.3 859,815 - -0.5 859,903 + -1.0 859,903 + -1.4 859,904 - -2.0 859,904 - -2.7 859,923 + -0.5 859,923 + +0.6 859,923 + -0.6 859,923 + -0.7 859,923 + +1.3 859,923 + -1.5 859,923 + -1.5 859,923 + -1.5 859,925 + +0.1 859,925 + +0.2 859,925 + -0.2 859,925 + -0.9 859,925 + -2.0 859,925 + +0.4 859,925 + -0.2 859,925 + +0.4 859,926 - -1.2 859,926 - -1.5 859,926 - +0.3 859,926 - -0.2 859,959 + +1.3 859,960 - +0.6 859,961 + -0.0 859,962 - +0.8 859,963 + +1.4 859,963 + +0.6 859,963 + +2.3 859,963 + -0.5 859,963 + +0.5 859,964 - +0.7 859,964 - +1.0 859,964 - +0.8 859,964 - -2.0 860,053 - +0.0 860,053 - +1.2 860,100 + -0.9 860,101 - -0.4 860,101 - -1.1 860,101 - -0.5 860,101 - -0.1 860,180 + Pf1N1B4_756 0.43 -0.2 860,181 - Pf1N1B4_756 0.43 -1.3 860,181 - Pf1N1B4_756 0.43 -1.5 860,181 - -1.2 860,183 - Pf1N1B4_756 0.44 -1.5 860,185 - Pf1N1B4_756 0.46 -1.3 860,372 + +0.8 860,558 - -0.3 860,569 - +1.3 860,708 + Pf1N1B4_757 0.12 -0.2 860,709 - Pf1N1B4_757 0.12 -0.0 860,719 + Pf1N1B4_757 0.13 +0.5 860,720 - Pf1N1B4_757 0.13 +0.6 860,777 + Pf1N1B4_757 0.17 +1.6 860,784 - Pf1N1B4_757 0.18 -1.4 860,784 - Pf1N1B4_757 0.18 -0.7 860,835 + Pf1N1B4_757 0.22 +0.5 860,835 + Pf1N1B4_757 0.22 -0.4 860,835 + Pf1N1B4_757 0.22 +0.8 860,835 + Pf1N1B4_757 0.22 -1.5 860,835 + Pf1N1B4_757 0.22 +0.3 860,835 + Pf1N1B4_757 0.22 +0.3 860,835 + Pf1N1B4_757 0.22 -2.1 860,836 - Pf1N1B4_757 0.22 -1.5 860,836 - -0.7 860,836 - Pf1N1B4_757 0.22 -0.1 860,836 - Pf1N1B4_757 0.22 +0.3 860,976 + -0.5 860,976 + Pf1N1B4_757 0.33 +1.5 861,037 - Pf1N1B4_757 0.38 -0.4 861,037 - Pf1N1B4_757 0.38 +1.6 861,037 - Pf1N1B4_757 0.38 -1.0 861,037 - Pf1N1B4_757 0.38 -1.2 861,049 + Pf1N1B4_757 0.39 +0.8 861,049 + Pf1N1B4_757 0.39 -2.3 861,049 + Pf1N1B4_757 0.39 -0.4 861,050 - Pf1N1B4_757 0.39 +0.1 861,050 - Pf1N1B4_757 0.39 +0.4 861,050 - Pf1N1B4_757 0.39 -2.7 861,079 - Pf1N1B4_757 0.41 -1.3 861,188 + Pf1N1B4_757 0.50 +0.4 861,189 - Pf1N1B4_757 0.50 -2.0 861,284 + Pf1N1B4_757 0.58 +1.3 861,284 + Pf1N1B4_757 0.58 +0.1 861,285 - Pf1N1B4_757 0.58 +0.9 861,285 - Pf1N1B4_757 0.58 -1.0 861,285 - Pf1N1B4_757 0.58 -1.2 861,285 - Pf1N1B4_757 0.58 -2.0 861,285 - Pf1N1B4_757 0.58 -0.2 861,285 - Pf1N1B4_757 0.58 +1.3 861,285 - Pf1N1B4_757 0.58 -0.5
Or see this region's nucleotide sequence