Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_71 and Pf1N1B4_72 are separated by 123 nucleotides Pf1N1B4_72 and Pf1N1B4_73 are separated by 142 nucleotides
Pf1N1B4_71 - Glucarate dehydratase (EC 4.2.1.40), at 74,280 to 75,623
_71
Pf1N1B4_72 - Transcriptional regulator, LysR family, at 75,747 to 76,619
_72
Pf1N1B4_73 - Beta-lactamase (EC 3.5.2.6), at 76,762 to 77,934
_73
Position (kb)
75
76
77 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 75.206 kb on - strand, within Pf1N1B4_71 at 75.210 kb on - strand, within Pf1N1B4_71 at 75.320 kb on + strand, within Pf1N1B4_71 at 75.523 kb on - strand at 75.523 kb on - strand at 75.523 kb on - strand at 75.523 kb on - strand at 75.745 kb on + strand at 75.745 kb on + strand at 75.745 kb on + strand at 75.745 kb on + strand at 75.745 kb on + strand at 75.746 kb on - strand at 75.746 kb on - strand at 75.746 kb on - strand at 75.746 kb on - strand at 75.749 kb on - strand at 75.749 kb on - strand at 75.829 kb on + strand at 76.251 kb on + strand, within Pf1N1B4_72 at 76.251 kb on + strand, within Pf1N1B4_72 at 76.252 kb on - strand, within Pf1N1B4_72 at 76.252 kb on - strand, within Pf1N1B4_72 at 76.656 kb on + strand at 76.657 kb on - strand at 76.688 kb on + strand at 76.689 kb on - strand at 76.689 kb on - strand at 76.689 kb on - strand at 76.689 kb on - strand at 76.786 kb on - strand at 76.813 kb on + strand at 76.814 kb on - strand at 76.973 kb on - strand, within Pf1N1B4_73 at 76.973 kb on - strand, within Pf1N1B4_73 at 77.071 kb on + strand, within Pf1N1B4_73 at 77.124 kb on - strand, within Pf1N1B4_73 at 77.132 kb on + strand, within Pf1N1B4_73 at 77.133 kb on - strand, within Pf1N1B4_73 at 77.133 kb on - strand, within Pf1N1B4_73 at 77.133 kb on - strand, within Pf1N1B4_73 at 77.133 kb on - strand, within Pf1N1B4_73 at 77.179 kb on + strand, within Pf1N1B4_73 at 77.179 kb on + strand, within Pf1N1B4_73 at 77.179 kb on + strand, within Pf1N1B4_73 at 77.180 kb on - strand, within Pf1N1B4_73 at 77.180 kb on - strand, within Pf1N1B4_73 at 77.245 kb on + strand, within Pf1N1B4_73 at 77.246 kb on - strand, within Pf1N1B4_73 at 77.293 kb on + strand, within Pf1N1B4_73 at 77.407 kb on + strand, within Pf1N1B4_73 at 77.408 kb on - strand, within Pf1N1B4_73 at 77.408 kb on - strand, within Pf1N1B4_73 at 77.408 kb on - strand, within Pf1N1B4_73 at 77.440 kb on + strand, within Pf1N1B4_73 at 77.452 kb on + strand, within Pf1N1B4_73
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 75,206 - Pf1N1B4_71 0.69 +1.0 75,210 - Pf1N1B4_71 0.69 +1.6 75,320 + Pf1N1B4_71 0.77 -0.8 75,523 - +1.6 75,523 - +1.2 75,523 - +0.6 75,523 - +0.3 75,745 + +0.5 75,745 + +1.2 75,745 + -1.0 75,745 + -0.1 75,745 + -1.0 75,746 - +0.0 75,746 - -1.0 75,746 - +0.6 75,746 - -2.5 75,749 - -1.7 75,749 - +0.7 75,829 + +0.3 76,251 + Pf1N1B4_72 0.58 -0.6 76,251 + Pf1N1B4_72 0.58 -1.3 76,252 - Pf1N1B4_72 0.58 -0.4 76,252 - Pf1N1B4_72 0.58 +1.6 76,656 + +1.6 76,657 - -0.9 76,688 + +1.3 76,689 - +0.2 76,689 - +0.4 76,689 - +0.6 76,689 - -1.2 76,786 - +1.0 76,813 + -0.2 76,814 - +0.4 76,973 - Pf1N1B4_73 0.18 +0.1 76,973 - Pf1N1B4_73 0.18 +0.6 77,071 + Pf1N1B4_73 0.26 +0.2 77,124 - Pf1N1B4_73 0.31 +1.5 77,132 + Pf1N1B4_73 0.32 +2.1 77,133 - Pf1N1B4_73 0.32 +0.1 77,133 - Pf1N1B4_73 0.32 +0.8 77,133 - Pf1N1B4_73 0.32 -1.0 77,133 - Pf1N1B4_73 0.32 +2.2 77,179 + Pf1N1B4_73 0.36 -0.2 77,179 + Pf1N1B4_73 0.36 -0.1 77,179 + Pf1N1B4_73 0.36 +0.8 77,180 - Pf1N1B4_73 0.36 -0.4 77,180 - Pf1N1B4_73 0.36 -1.7 77,245 + Pf1N1B4_73 0.41 -0.0 77,246 - Pf1N1B4_73 0.41 +0.9 77,293 + Pf1N1B4_73 0.45 -0.2 77,407 + Pf1N1B4_73 0.55 -1.1 77,408 - Pf1N1B4_73 0.55 +0.6 77,408 - Pf1N1B4_73 0.55 -1.7 77,408 - Pf1N1B4_73 0.55 +0.2 77,440 + Pf1N1B4_73 0.58 +2.6 77,452 + Pf1N1B4_73 0.59 -0.4
Or see this region's nucleotide sequence