Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_69 and Pf1N1B4_70 are separated by 388 nucleotides Pf1N1B4_70 and Pf1N1B4_71 are separated by 23 nucleotides
Pf1N1B4_69 - Predicted D-glucarate or D-galactorate regulator, GntR family, at 71,829 to 72,542
_69
Pf1N1B4_70 - Major facilitator family transporter, at 72,931 to 74,256
_70
Pf1N1B4_71 - Glucarate dehydratase (EC 4.2.1.40), at 74,280 to 75,623
_71
Position (kb) 72
73
74
75 Strain fitness (log2 ratio) -2
-1
0
1
2 at 72.028 kb on - strand, within Pf1N1B4_69 at 72.028 kb on - strand, within Pf1N1B4_69 at 72.139 kb on + strand, within Pf1N1B4_69 at 72.140 kb on - strand, within Pf1N1B4_69 at 72.303 kb on + strand, within Pf1N1B4_69 at 72.304 kb on - strand, within Pf1N1B4_69 at 72.577 kb on - strand at 72.648 kb on - strand at 72.749 kb on - strand at 72.915 kb on + strand at 72.916 kb on - strand at 72.916 kb on - strand at 72.916 kb on - strand at 73.024 kb on + strand at 73.024 kb on + strand at 73.024 kb on + strand at 73.024 kb on + strand at 73.024 kb on + strand at 73.024 kb on + strand at 73.025 kb on - strand at 73.025 kb on - strand at 73.262 kb on - strand, within Pf1N1B4_70 at 73.318 kb on + strand at 73.319 kb on - strand, within Pf1N1B4_70 at 73.319 kb on - strand, within Pf1N1B4_70 at 73.321 kb on + strand, within Pf1N1B4_70 at 73.321 kb on + strand, within Pf1N1B4_70 at 73.322 kb on - strand, within Pf1N1B4_70 at 73.392 kb on - strand at 73.471 kb on + strand, within Pf1N1B4_70 at 73.471 kb on + strand at 73.471 kb on + strand, within Pf1N1B4_70 at 73.472 kb on - strand, within Pf1N1B4_70 at 73.472 kb on - strand, within Pf1N1B4_70 at 73.472 kb on - strand at 73.690 kb on + strand, within Pf1N1B4_70 at 73.690 kb on + strand, within Pf1N1B4_70 at 73.690 kb on + strand, within Pf1N1B4_70 at 73.690 kb on + strand, within Pf1N1B4_70 at 73.691 kb on - strand, within Pf1N1B4_70 at 73.691 kb on - strand, within Pf1N1B4_70 at 73.715 kb on - strand, within Pf1N1B4_70 at 73.825 kb on + strand, within Pf1N1B4_70 at 73.825 kb on + strand, within Pf1N1B4_70 at 73.825 kb on + strand, within Pf1N1B4_70 at 73.825 kb on + strand, within Pf1N1B4_70 at 73.826 kb on - strand, within Pf1N1B4_70 at 73.826 kb on - strand, within Pf1N1B4_70 at 73.826 kb on - strand, within Pf1N1B4_70 at 73.826 kb on - strand, within Pf1N1B4_70 at 74.015 kb on - strand, within Pf1N1B4_70 at 74.244 kb on + strand at 74.245 kb on - strand at 74.245 kb on - strand at 74.245 kb on - strand at 74.245 kb on - strand at 74.245 kb on - strand at 74.245 kb on - strand at 74.268 kb on - strand at 74.465 kb on + strand, within Pf1N1B4_71 at 74.523 kb on + strand, within Pf1N1B4_71 at 74.742 kb on + strand, within Pf1N1B4_71 at 74.742 kb on + strand, within Pf1N1B4_71 at 74.742 kb on + strand, within Pf1N1B4_71 at 74.742 kb on + strand, within Pf1N1B4_71 at 74.743 kb on - strand, within Pf1N1B4_71 at 74.743 kb on - strand, within Pf1N1B4_71 at 75.206 kb on - strand, within Pf1N1B4_71 at 75.210 kb on - strand, within Pf1N1B4_71
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 72,028 - Pf1N1B4_69 0.28 -1.2 72,028 - Pf1N1B4_69 0.28 -1.8 72,139 + Pf1N1B4_69 0.43 -1.0 72,140 - Pf1N1B4_69 0.44 -0.7 72,303 + Pf1N1B4_69 0.66 -0.4 72,304 - Pf1N1B4_69 0.67 -1.4 72,577 - -0.1 72,648 - -0.8 72,749 - -0.9 72,915 + +0.0 72,916 - -0.7 72,916 - +0.7 72,916 - -0.4 73,024 + +0.2 73,024 + -1.7 73,024 + -0.8 73,024 + +1.1 73,024 + -1.7 73,024 + -1.7 73,025 - +0.6 73,025 - -0.2 73,262 - Pf1N1B4_70 0.25 -0.8 73,318 + -0.4 73,319 - Pf1N1B4_70 0.29 +0.2 73,319 - Pf1N1B4_70 0.29 -0.1 73,321 + Pf1N1B4_70 0.29 +0.8 73,321 + Pf1N1B4_70 0.29 +0.9 73,322 - Pf1N1B4_70 0.29 +0.3 73,392 - -1.4 73,471 + Pf1N1B4_70 0.41 +2.0 73,471 + +1.8 73,471 + Pf1N1B4_70 0.41 +0.4 73,472 - Pf1N1B4_70 0.41 +0.6 73,472 - Pf1N1B4_70 0.41 +0.2 73,472 - -1.4 73,690 + Pf1N1B4_70 0.57 -1.4 73,690 + Pf1N1B4_70 0.57 +1.2 73,690 + Pf1N1B4_70 0.57 +0.9 73,690 + Pf1N1B4_70 0.57 +0.9 73,691 - Pf1N1B4_70 0.57 +0.3 73,691 - Pf1N1B4_70 0.57 -0.1 73,715 - Pf1N1B4_70 0.59 -0.7 73,825 + Pf1N1B4_70 0.67 -0.1 73,825 + Pf1N1B4_70 0.67 +0.3 73,825 + Pf1N1B4_70 0.67 -1.2 73,825 + Pf1N1B4_70 0.67 -0.1 73,826 - Pf1N1B4_70 0.67 +1.2 73,826 - Pf1N1B4_70 0.67 -1.1 73,826 - Pf1N1B4_70 0.67 +0.6 73,826 - Pf1N1B4_70 0.67 -1.0 74,015 - Pf1N1B4_70 0.82 -1.1 74,244 + -1.1 74,245 - +1.1 74,245 - -2.4 74,245 - -2.0 74,245 - -0.9 74,245 - -0.4 74,245 - +0.4 74,268 - +1.0 74,465 + Pf1N1B4_71 0.14 +0.0 74,523 + Pf1N1B4_71 0.18 -0.1 74,742 + Pf1N1B4_71 0.34 -0.4 74,742 + Pf1N1B4_71 0.34 -0.8 74,742 + Pf1N1B4_71 0.34 +0.2 74,742 + Pf1N1B4_71 0.34 +0.6 74,743 - Pf1N1B4_71 0.34 -0.4 74,743 - Pf1N1B4_71 0.34 -0.8 75,206 - Pf1N1B4_71 0.69 +1.0 75,210 - Pf1N1B4_71 0.69 +1.6
Or see this region's nucleotide sequence