Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_681 and Pf1N1B4_682 are separated by 361 nucleotides Pf1N1B4_682 and Pf1N1B4_683 are separated by 42 nucleotides Pf1N1B4_683 and Pf1N1B4_684 are separated by 197 nucleotides
Pf1N1B4_681 - FOG: GGDEF domain, at 778,079 to 779,185
_681
Pf1N1B4_682 - hypothetical protein, at 779,547 to 779,699
_682
Pf1N1B4_683 - Fumarate hydratase class I, aerobic (EC 4.2.1.2), at 779,742 to 781,265
_683
Pf1N1B4_684 - C4-dicarboxylate transport sensor protein, at 781,463 to 783,265
_684
Position (kb)
779
780
781
782 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 778.873 kb on - strand, within Pf1N1B4_681 at 778.873 kb on - strand, within Pf1N1B4_681 at 778.947 kb on - strand, within Pf1N1B4_681 at 778.947 kb on - strand, within Pf1N1B4_681 at 779.076 kb on + strand at 779.077 kb on - strand at 779.125 kb on - strand at 779.125 kb on - strand at 779.125 kb on - strand at 779.226 kb on + strand at 779.226 kb on + strand at 779.226 kb on + strand at 779.227 kb on - strand at 779.227 kb on - strand at 779.231 kb on + strand at 779.232 kb on - strand at 779.232 kb on - strand at 779.265 kb on + strand at 779.266 kb on - strand at 779.328 kb on - strand at 779.407 kb on - strand at 779.407 kb on - strand at 779.407 kb on - strand at 779.407 kb on - strand at 779.474 kb on + strand at 779.474 kb on + strand at 779.474 kb on + strand at 779.474 kb on + strand at 779.474 kb on + strand at 779.474 kb on + strand at 779.474 kb on + strand at 779.474 kb on + strand at 779.474 kb on + strand at 779.475 kb on - strand at 779.475 kb on - strand at 779.475 kb on - strand at 779.475 kb on - strand at 779.475 kb on - strand at 779.475 kb on - strand at 779.475 kb on - strand at 779.544 kb on + strand at 779.544 kb on + strand at 779.545 kb on - strand at 779.545 kb on - strand at 779.612 kb on + strand, within Pf1N1B4_682 at 779.613 kb on - strand, within Pf1N1B4_682 at 779.707 kb on - strand at 779.806 kb on - strand at 779.808 kb on + strand at 779.848 kb on - strand at 779.848 kb on - strand at 779.848 kb on - strand at 780.049 kb on - strand, within Pf1N1B4_683 at 780.169 kb on + strand, within Pf1N1B4_683 at 780.259 kb on + strand, within Pf1N1B4_683 at 780.858 kb on + strand, within Pf1N1B4_683 at 780.858 kb on + strand, within Pf1N1B4_683 at 780.858 kb on + strand, within Pf1N1B4_683 at 780.859 kb on - strand, within Pf1N1B4_683 at 780.861 kb on + strand at 780.861 kb on + strand, within Pf1N1B4_683 at 780.861 kb on + strand, within Pf1N1B4_683 at 780.862 kb on - strand, within Pf1N1B4_683 at 781.068 kb on + strand, within Pf1N1B4_683 at 781.137 kb on + strand at 781.263 kb on + strand at 781.351 kb on + strand at 781.351 kb on + strand at 781.352 kb on + strand at 781.352 kb on - strand at 781.352 kb on - strand at 781.352 kb on - strand at 781.352 kb on - strand at 781.378 kb on + strand at 781.379 kb on - strand at 781.379 kb on - strand at 781.381 kb on - strand at 781.411 kb on - strand at 781.420 kb on - strand at 781.467 kb on + strand at 781.468 kb on - strand at 781.468 kb on - strand at 781.523 kb on - strand at 781.541 kb on + strand at 781.541 kb on + strand at 781.542 kb on - strand at 781.542 kb on - strand at 781.655 kb on + strand, within Pf1N1B4_684 at 781.655 kb on + strand, within Pf1N1B4_684 at 781.655 kb on + strand, within Pf1N1B4_684 at 781.655 kb on + strand, within Pf1N1B4_684 at 781.656 kb on - strand, within Pf1N1B4_684 at 781.656 kb on - strand, within Pf1N1B4_684 at 781.688 kb on + strand, within Pf1N1B4_684 at 781.728 kb on + strand, within Pf1N1B4_684 at 781.887 kb on - strand, within Pf1N1B4_684 at 781.887 kb on - strand, within Pf1N1B4_684 at 781.917 kb on - strand, within Pf1N1B4_684 at 781.988 kb on + strand, within Pf1N1B4_684 at 782.216 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 778,873 - Pf1N1B4_681 0.72 +0.2 778,873 - Pf1N1B4_681 0.72 +0.2 778,947 - Pf1N1B4_681 0.78 -0.1 778,947 - Pf1N1B4_681 0.78 +0.8 779,076 + +0.4 779,077 - -0.0 779,125 - -0.8 779,125 - +0.0 779,125 - -0.2 779,226 + +0.0 779,226 + +0.4 779,226 + +0.3 779,227 - +0.5 779,227 - +0.6 779,231 + +0.5 779,232 - -0.2 779,232 - +0.0 779,265 + +1.9 779,266 - -0.5 779,328 - +0.1 779,407 - +1.7 779,407 - -0.8 779,407 - +0.8 779,407 - -0.3 779,474 + -0.6 779,474 + -1.5 779,474 + +0.8 779,474 + +0.2 779,474 + +0.8 779,474 + -0.2 779,474 + -0.9 779,474 + -1.5 779,474 + +1.5 779,475 - +0.7 779,475 - +1.2 779,475 - +0.4 779,475 - +0.8 779,475 - +0.4 779,475 - -0.8 779,475 - -0.4 779,544 + +0.8 779,544 + +0.1 779,545 - -1.8 779,545 - -1.3 779,612 + Pf1N1B4_682 0.42 -0.8 779,613 - Pf1N1B4_682 0.43 -0.2 779,707 - -1.3 779,806 - -1.8 779,808 + +1.2 779,848 - -0.5 779,848 - -1.8 779,848 - -1.0 780,049 - Pf1N1B4_683 0.20 -2.6 780,169 + Pf1N1B4_683 0.28 -1.8 780,259 + Pf1N1B4_683 0.34 -1.0 780,858 + Pf1N1B4_683 0.73 -1.6 780,858 + Pf1N1B4_683 0.73 -1.5 780,858 + Pf1N1B4_683 0.73 -0.6 780,859 - Pf1N1B4_683 0.73 +0.8 780,861 + +0.8 780,861 + Pf1N1B4_683 0.73 +1.8 780,861 + Pf1N1B4_683 0.73 -1.2 780,862 - Pf1N1B4_683 0.73 -2.0 781,068 + Pf1N1B4_683 0.87 -0.2 781,137 + -0.8 781,263 + -0.7 781,351 + +0.3 781,351 + +0.1 781,352 + +0.8 781,352 - +0.4 781,352 - -0.5 781,352 - -0.3 781,352 - -0.5 781,378 + -1.0 781,379 - +1.1 781,379 - +0.6 781,381 - -0.2 781,411 - +0.6 781,420 - +0.1 781,467 + -0.4 781,468 - +0.8 781,468 - +0.5 781,523 - +1.1 781,541 + +0.8 781,541 + -0.9 781,542 - +1.3 781,542 - -0.6 781,655 + Pf1N1B4_684 0.11 +0.2 781,655 + Pf1N1B4_684 0.11 +0.6 781,655 + Pf1N1B4_684 0.11 -0.4 781,655 + Pf1N1B4_684 0.11 +0.3 781,656 - Pf1N1B4_684 0.11 -0.8 781,656 - Pf1N1B4_684 0.11 +0.2 781,688 + Pf1N1B4_684 0.12 -0.6 781,728 + Pf1N1B4_684 0.15 +0.8 781,887 - Pf1N1B4_684 0.24 +0.4 781,887 - Pf1N1B4_684 0.24 +0.2 781,917 - Pf1N1B4_684 0.25 +2.1 781,988 + Pf1N1B4_684 0.29 +0.4 782,216 + +0.8
Or see this region's nucleotide sequence