Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_674 and Pf1N1B4_675 are separated by 854 nucleotides Pf1N1B4_675 and Pf1N1B4_676 are separated by 146 nucleotides Pf1N1B4_676 and Pf1N1B4_677 are separated by 222 nucleotides
Pf1N1B4_674 - POSSIBLE CONSERVED MEMBRANE PROTEIN, at 770,943 to 772,274
_674
Pf1N1B4_675 - hypothetical protein, at 773,129 to 773,347
_675
Pf1N1B4_676 - FIG00953991: hypothetical protein, at 773,494 to 773,853
_676
Pf1N1B4_677 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 774,076 to 774,390
_677
Position (kb) 773
774 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 772.140 kb on + strand, within Pf1N1B4_674 at 772.140 kb on + strand, within Pf1N1B4_674 at 772.141 kb on - strand at 772.142 kb on + strand at 772.142 kb on + strand at 772.143 kb on - strand at 772.143 kb on - strand at 772.143 kb on - strand at 772.143 kb on - strand at 772.143 kb on - strand at 772.150 kb on + strand at 772.150 kb on + strand at 772.150 kb on + strand at 772.243 kb on - strand at 772.244 kb on + strand at 772.244 kb on + strand at 772.245 kb on - strand at 772.272 kb on + strand at 772.272 kb on + strand at 772.273 kb on - strand at 772.287 kb on + strand at 772.294 kb on + strand at 772.295 kb on - strand at 772.309 kb on - strand at 772.314 kb on + strand at 772.314 kb on + strand at 772.315 kb on - strand at 772.315 kb on - strand at 772.315 kb on - strand at 772.331 kb on + strand at 772.332 kb on - strand at 772.332 kb on - strand at 772.332 kb on - strand at 772.332 kb on - strand at 772.332 kb on - strand at 772.337 kb on + strand at 772.337 kb on + strand at 772.338 kb on - strand at 772.338 kb on - strand at 772.338 kb on - strand at 772.338 kb on - strand at 772.355 kb on - strand at 772.359 kb on - strand at 772.359 kb on - strand at 772.388 kb on + strand at 772.388 kb on + strand at 772.395 kb on - strand at 772.395 kb on - strand at 772.428 kb on + strand at 772.429 kb on - strand at 772.485 kb on + strand at 772.597 kb on + strand at 772.651 kb on + strand at 772.652 kb on - strand at 772.672 kb on + strand at 772.672 kb on + strand at 772.672 kb on + strand at 772.673 kb on - strand at 772.673 kb on - strand at 772.716 kb on + strand at 772.717 kb on - strand at 772.717 kb on - strand at 772.720 kb on - strand at 772.734 kb on - strand at 772.748 kb on - strand at 772.825 kb on - strand at 772.852 kb on + strand at 772.852 kb on + strand at 772.852 kb on + strand at 772.852 kb on + strand at 772.852 kb on + strand at 772.894 kb on + strand at 772.894 kb on + strand at 772.894 kb on + strand at 772.894 kb on + strand at 772.894 kb on + strand at 772.895 kb on - strand at 772.895 kb on - strand at 772.895 kb on - strand at 772.895 kb on - strand at 772.895 kb on - strand at 772.922 kb on + strand at 773.066 kb on + strand at 773.067 kb on - strand at 773.086 kb on + strand at 773.187 kb on - strand, within Pf1N1B4_675 at 773.187 kb on - strand, within Pf1N1B4_675 at 773.187 kb on - strand, within Pf1N1B4_675 at 773.274 kb on - strand, within Pf1N1B4_675 at 773.274 kb on - strand, within Pf1N1B4_675 at 773.274 kb on - strand, within Pf1N1B4_675 at 773.345 kb on + strand at 773.346 kb on - strand at 773.352 kb on + strand at 773.352 kb on + strand at 773.353 kb on - strand at 773.357 kb on - strand at 773.357 kb on - strand at 773.357 kb on - strand at 773.425 kb on + strand at 773.425 kb on + strand at 773.426 kb on - strand at 773.426 kb on - strand at 773.426 kb on - strand at 773.426 kb on - strand at 773.426 kb on - strand at 773.474 kb on + strand at 773.475 kb on - strand at 773.528 kb on + strand at 773.529 kb on - strand at 773.529 kb on - strand at 773.597 kb on + strand, within Pf1N1B4_676 at 773.597 kb on + strand, within Pf1N1B4_676 at 773.597 kb on + strand, within Pf1N1B4_676 at 773.598 kb on - strand, within Pf1N1B4_676 at 773.598 kb on - strand, within Pf1N1B4_676 at 773.598 kb on - strand, within Pf1N1B4_676 at 773.698 kb on - strand, within Pf1N1B4_676 at 773.761 kb on - strand, within Pf1N1B4_676 at 773.813 kb on + strand, within Pf1N1B4_676 at 773.896 kb on + strand at 773.897 kb on - strand at 773.918 kb on - strand at 773.918 kb on - strand at 773.925 kb on + strand at 773.925 kb on + strand at 773.925 kb on + strand at 773.925 kb on + strand at 773.926 kb on - strand at 773.926 kb on - strand at 773.928 kb on - strand at 773.928 kb on - strand at 773.928 kb on - strand at 773.928 kb on - strand at 773.986 kb on + strand at 774.008 kb on + strand at 774.009 kb on - strand at 774.018 kb on + strand at 774.019 kb on - strand at 774.050 kb on + strand at 774.052 kb on + strand at 774.052 kb on + strand at 774.052 kb on + strand at 774.052 kb on + strand at 774.052 kb on + strand at 774.052 kb on + strand at 774.052 kb on + strand at 774.052 kb on + strand at 774.052 kb on + strand at 774.052 kb on + strand at 774.052 kb on + strand at 774.053 kb on - strand at 774.053 kb on - strand at 774.053 kb on - strand at 774.053 kb on - strand at 774.053 kb on - strand at 774.053 kb on - strand at 774.053 kb on - strand at 774.053 kb on - strand at 774.053 kb on - strand at 774.053 kb on - strand at 774.207 kb on - strand, within Pf1N1B4_677 at 774.207 kb on - strand, within Pf1N1B4_677 at 774.207 kb on - strand, within Pf1N1B4_677 at 774.226 kb on - strand, within Pf1N1B4_677 at 774.284 kb on + strand, within Pf1N1B4_677 at 774.284 kb on + strand, within Pf1N1B4_677 at 774.284 kb on + strand, within Pf1N1B4_677 at 774.284 kb on + strand, within Pf1N1B4_677 at 774.284 kb on + strand, within Pf1N1B4_677 at 774.284 kb on + strand, within Pf1N1B4_677 at 774.285 kb on - strand, within Pf1N1B4_677 at 774.285 kb on - strand, within Pf1N1B4_677 at 774.285 kb on - strand, within Pf1N1B4_677 at 774.285 kb on - strand, within Pf1N1B4_677 at 774.285 kb on - strand, within Pf1N1B4_677 at 774.285 kb on - strand, within Pf1N1B4_677 at 774.285 kb on - strand, within Pf1N1B4_677 at 774.285 kb on - strand, within Pf1N1B4_677
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 772,140 + Pf1N1B4_674 0.90 +0.3 772,140 + Pf1N1B4_674 0.90 +1.2 772,141 - -0.8 772,142 + +0.2 772,142 + -0.2 772,143 - +1.0 772,143 - -0.4 772,143 - +0.3 772,143 - +1.2 772,143 - +0.0 772,150 + +0.2 772,150 + +0.3 772,150 + -0.2 772,243 - +0.1 772,244 + +0.6 772,244 + -1.3 772,245 - -1.7 772,272 + -1.2 772,272 + +0.2 772,273 - +0.3 772,287 + +1.1 772,294 + +0.6 772,295 - -1.5 772,309 - +0.4 772,314 + -0.7 772,314 + -2.0 772,315 - +0.1 772,315 - +0.5 772,315 - +0.5 772,331 + -0.2 772,332 - +1.3 772,332 - +1.3 772,332 - +1.1 772,332 - +0.4 772,332 - -0.2 772,337 + +1.7 772,337 + -0.2 772,338 - +0.0 772,338 - +0.2 772,338 - +0.2 772,338 - +0.8 772,355 - -1.0 772,359 - -0.2 772,359 - +0.5 772,388 + -0.8 772,388 + +0.8 772,395 - +0.1 772,395 - -0.2 772,428 + +1.4 772,429 - +0.8 772,485 + -0.3 772,597 + +2.0 772,651 + -0.6 772,652 - -0.0 772,672 + +1.1 772,672 + +0.5 772,672 + +0.3 772,673 - +0.2 772,673 - -0.1 772,716 + +0.8 772,717 - +0.6 772,717 - +1.0 772,720 - +0.1 772,734 - -0.4 772,748 - -1.8 772,825 - +0.1 772,852 + -1.8 772,852 + +1.5 772,852 + +0.3 772,852 + -1.0 772,852 + +0.8 772,894 + +1.8 772,894 + +0.1 772,894 + +0.7 772,894 + -2.2 772,894 + +0.5 772,895 - -0.6 772,895 - +0.1 772,895 - -0.5 772,895 - -0.2 772,895 - +0.3 772,922 + +0.8 773,066 + +0.2 773,067 - +0.2 773,086 + -0.3 773,187 - Pf1N1B4_675 0.26 +0.0 773,187 - Pf1N1B4_675 0.26 -2.9 773,187 - Pf1N1B4_675 0.26 -1.2 773,274 - Pf1N1B4_675 0.66 -2.8 773,274 - Pf1N1B4_675 0.66 +0.8 773,274 - Pf1N1B4_675 0.66 -1.2 773,345 + +0.3 773,346 - -0.8 773,352 + -0.5 773,352 + +0.8 773,353 - -1.2 773,357 - -0.8 773,357 - +2.8 773,357 - -0.2 773,425 + +0.2 773,425 + +0.8 773,426 - +0.2 773,426 - -0.8 773,426 - +0.4 773,426 - -0.5 773,426 - -2.7 773,474 + +0.8 773,475 - -0.3 773,528 + -1.8 773,529 - -1.4 773,529 - +0.8 773,597 + Pf1N1B4_676 0.29 +0.4 773,597 + Pf1N1B4_676 0.29 +0.2 773,597 + Pf1N1B4_676 0.29 +0.6 773,598 - Pf1N1B4_676 0.29 +0.2 773,598 - Pf1N1B4_676 0.29 +0.6 773,598 - Pf1N1B4_676 0.29 +1.1 773,698 - Pf1N1B4_676 0.57 +0.8 773,761 - Pf1N1B4_676 0.74 +0.4 773,813 + Pf1N1B4_676 0.89 -1.4 773,896 + -1.0 773,897 - +0.6 773,918 - +1.2 773,918 - -1.4 773,925 + -0.5 773,925 + +3.6 773,925 + -0.9 773,925 + +0.1 773,926 - -0.6 773,926 - -0.0 773,928 - +0.6 773,928 - -1.0 773,928 - +0.8 773,928 - +0.0 773,986 + +0.4 774,008 + +1.4 774,009 - -1.6 774,018 + +1.0 774,019 - +0.5 774,050 + +0.3 774,052 + -0.5 774,052 + +0.8 774,052 + -0.1 774,052 + -0.2 774,052 + -0.8 774,052 + -1.2 774,052 + -0.6 774,052 + +0.5 774,052 + +0.2 774,052 + +1.1 774,052 + +1.6 774,053 - +0.4 774,053 - -1.2 774,053 - -0.4 774,053 - +0.5 774,053 - +0.7 774,053 - -0.9 774,053 - +1.2 774,053 - -0.2 774,053 - +0.2 774,053 - -0.9 774,207 - Pf1N1B4_677 0.42 +1.3 774,207 - Pf1N1B4_677 0.42 -0.6 774,207 - Pf1N1B4_677 0.42 -0.2 774,226 - Pf1N1B4_677 0.48 +0.4 774,284 + Pf1N1B4_677 0.66 -1.0 774,284 + Pf1N1B4_677 0.66 +0.3 774,284 + Pf1N1B4_677 0.66 +1.4 774,284 + Pf1N1B4_677 0.66 -0.2 774,284 + Pf1N1B4_677 0.66 +0.4 774,284 + Pf1N1B4_677 0.66 +0.8 774,285 - Pf1N1B4_677 0.66 +0.8 774,285 - Pf1N1B4_677 0.66 -0.2 774,285 - Pf1N1B4_677 0.66 +0.1 774,285 - Pf1N1B4_677 0.66 -0.6 774,285 - Pf1N1B4_677 0.66 +1.2 774,285 - Pf1N1B4_677 0.66 -0.8 774,285 - Pf1N1B4_677 0.66 -0.5 774,285 - Pf1N1B4_677 0.66 +0.1
Or see this region's nucleotide sequence