Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_616 and Pf1N1B4_617 are separated by 191 nucleotides Pf1N1B4_617 and Pf1N1B4_618 are separated by 12 nucleotides Pf1N1B4_618 and Pf1N1B4_619 are separated by 216 nucleotides
Pf1N1B4_616 - Ornithine carbamoyltransferase (EC 2.1.3.3), at 712,542 to 713,552
_616
Pf1N1B4_617 - Carbamate kinase (EC 2.7.2.2), at 713,744 to 714,673
_617
Pf1N1B4_618 - Acetyl-CoA carboxylase alpha subunit, at 714,686 to 715,048
_618
Pf1N1B4_619 - Sigma-54 dependent transcriptional regulator/sensory box protein, at 715,265 to 716,773
_619
Position (kb) 713
714
715 Strain fitness (log2 ratio) -2
-1
0
1 at 712.770 kb on + strand, within Pf1N1B4_616 at 712.770 kb on + strand, within Pf1N1B4_616 at 712.771 kb on - strand, within Pf1N1B4_616 at 712.771 kb on - strand, within Pf1N1B4_616 at 713.550 kb on + strand at 713.575 kb on + strand at 713.576 kb on - strand at 713.744 kb on - strand at 713.933 kb on + strand, within Pf1N1B4_617 at 713.996 kb on + strand at 714.126 kb on - strand, within Pf1N1B4_617 at 714.126 kb on - strand at 714.315 kb on - strand, within Pf1N1B4_617 at 714.315 kb on - strand, within Pf1N1B4_617 at 714.660 kb on + strand at 714.661 kb on - strand at 714.661 kb on - strand at 714.661 kb on - strand at 714.661 kb on - strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.665 kb on + strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.666 kb on - strand at 714.671 kb on + strand at 714.671 kb on + strand at 714.671 kb on + strand at 714.672 kb on - strand at 714.672 kb on - strand at 714.672 kb on - strand at 714.672 kb on - strand at 714.752 kb on + strand at 714.752 kb on + strand, within Pf1N1B4_618 at 714.752 kb on + strand at 714.770 kb on + strand, within Pf1N1B4_618 at 714.770 kb on + strand, within Pf1N1B4_618 at 714.770 kb on + strand, within Pf1N1B4_618 at 714.770 kb on + strand at 714.770 kb on + strand at 714.770 kb on + strand, within Pf1N1B4_618 at 714.771 kb on - strand, within Pf1N1B4_618 at 714.771 kb on - strand at 714.771 kb on - strand, within Pf1N1B4_618 at 714.771 kb on - strand, within Pf1N1B4_618 at 714.771 kb on - strand, within Pf1N1B4_618 at 714.771 kb on - strand, within Pf1N1B4_618 at 714.807 kb on + strand, within Pf1N1B4_618 at 714.881 kb on + strand, within Pf1N1B4_618 at 714.881 kb on + strand, within Pf1N1B4_618 at 714.881 kb on + strand, within Pf1N1B4_618 at 714.881 kb on + strand, within Pf1N1B4_618 at 714.881 kb on + strand, within Pf1N1B4_618 at 714.920 kb on + strand, within Pf1N1B4_618 at 714.975 kb on + strand, within Pf1N1B4_618 at 715.073 kb on + strand at 715.227 kb on + strand at 715.227 kb on + strand at 715.228 kb on - strand at 715.228 kb on - strand at 715.379 kb on + strand at 715.380 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 712,770 + Pf1N1B4_616 0.23 +0.8 712,770 + Pf1N1B4_616 0.23 +0.5 712,771 - Pf1N1B4_616 0.23 -0.4 712,771 - Pf1N1B4_616 0.23 -1.2 713,550 + -0.8 713,575 + -0.2 713,576 - -0.4 713,744 - +0.1 713,933 + Pf1N1B4_617 0.20 -2.2 713,996 + +0.4 714,126 - Pf1N1B4_617 0.41 -0.6 714,126 - +0.2 714,315 - Pf1N1B4_617 0.61 +0.2 714,315 - Pf1N1B4_617 0.61 +0.2 714,660 + +0.3 714,661 - +0.2 714,661 - -0.2 714,661 - +0.5 714,661 - +0.2 714,665 + +0.1 714,665 + -0.5 714,665 + +0.7 714,665 + -0.8 714,665 + -1.3 714,665 + +1.6 714,665 + +1.1 714,665 + -0.5 714,665 + +0.5 714,665 + -0.1 714,665 + -0.4 714,665 + -1.2 714,665 + +0.4 714,665 + -1.4 714,665 + -1.2 714,665 + +0.8 714,665 + +0.2 714,666 - -0.2 714,666 - -1.5 714,666 - +0.8 714,666 - +0.0 714,666 - -1.2 714,666 - +0.0 714,666 - -0.2 714,666 - +0.8 714,666 - +0.5 714,671 + +1.0 714,671 + -0.1 714,671 + -1.0 714,672 - +0.8 714,672 - -0.8 714,672 - +0.2 714,672 - -1.8 714,752 + -0.8 714,752 + Pf1N1B4_618 0.18 -0.8 714,752 + -0.2 714,770 + Pf1N1B4_618 0.23 +0.3 714,770 + Pf1N1B4_618 0.23 -0.5 714,770 + Pf1N1B4_618 0.23 +1.0 714,770 + +0.8 714,770 + +1.4 714,770 + Pf1N1B4_618 0.23 +0.0 714,771 - Pf1N1B4_618 0.23 -0.1 714,771 - -0.0 714,771 - Pf1N1B4_618 0.23 +0.4 714,771 - Pf1N1B4_618 0.23 -0.2 714,771 - Pf1N1B4_618 0.23 -0.4 714,771 - Pf1N1B4_618 0.23 -1.4 714,807 + Pf1N1B4_618 0.33 +0.8 714,881 + Pf1N1B4_618 0.54 +1.6 714,881 + Pf1N1B4_618 0.54 -0.5 714,881 + Pf1N1B4_618 0.54 -0.2 714,881 + Pf1N1B4_618 0.54 -0.8 714,881 + Pf1N1B4_618 0.54 -0.2 714,920 + Pf1N1B4_618 0.64 -0.8 714,975 + Pf1N1B4_618 0.80 -1.2 715,073 + -0.5 715,227 + +0.8 715,227 + +0.0 715,228 - +0.1 715,228 - -0.3 715,379 + +0.5 715,380 - +1.4
Or see this region's nucleotide sequence