Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_614 and Pf1N1B4_615 are separated by 35 nucleotides Pf1N1B4_615 and Pf1N1B4_616 are separated by 69 nucleotides
Pf1N1B4_614 - Arginine/ornithine antiporter ArcD, at 709,753 to 711,180
_614
Pf1N1B4_615 - Arginine deiminase (EC 3.5.3.6), at 711,216 to 712,472
_615
Pf1N1B4_616 - Ornithine carbamoyltransferase (EC 2.1.3.3), at 712,542 to 713,552
_616
Position (kb) 711
712
713 Strain fitness (log2 ratio) -2
-1
0
1
2 at 710.816 kb on - strand, within Pf1N1B4_614 at 710.830 kb on + strand, within Pf1N1B4_614 at 710.861 kb on - strand, within Pf1N1B4_614 at 710.911 kb on + strand, within Pf1N1B4_614 at 710.912 kb on - strand at 710.912 kb on - strand, within Pf1N1B4_614 at 710.971 kb on + strand, within Pf1N1B4_614 at 710.971 kb on + strand, within Pf1N1B4_614 at 710.990 kb on - strand, within Pf1N1B4_614 at 711.031 kb on + strand, within Pf1N1B4_614 at 711.031 kb on + strand, within Pf1N1B4_614 at 711.123 kb on - strand at 711.123 kb on - strand at 711.214 kb on + strand at 711.237 kb on + strand at 711.756 kb on + strand, within Pf1N1B4_615 at 711.757 kb on - strand, within Pf1N1B4_615 at 712.344 kb on + strand at 712.344 kb on + strand, within Pf1N1B4_615 at 712.345 kb on - strand, within Pf1N1B4_615 at 712.345 kb on - strand, within Pf1N1B4_615 at 712.345 kb on - strand, within Pf1N1B4_615 at 712.467 kb on + strand at 712.468 kb on - strand at 712.608 kb on + strand at 712.609 kb on - strand at 712.647 kb on + strand, within Pf1N1B4_616 at 712.648 kb on - strand, within Pf1N1B4_616 at 712.648 kb on - strand, within Pf1N1B4_616 at 712.770 kb on + strand, within Pf1N1B4_616 at 712.770 kb on + strand, within Pf1N1B4_616 at 712.771 kb on - strand, within Pf1N1B4_616 at 712.771 kb on - strand, within Pf1N1B4_616
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 710,816 - Pf1N1B4_614 0.74 +2.6 710,830 + Pf1N1B4_614 0.75 +1.5 710,861 - Pf1N1B4_614 0.78 -0.8 710,911 + Pf1N1B4_614 0.81 +0.4 710,912 - +0.8 710,912 - Pf1N1B4_614 0.81 +2.3 710,971 + Pf1N1B4_614 0.85 +0.4 710,971 + Pf1N1B4_614 0.85 +0.8 710,990 - Pf1N1B4_614 0.87 +0.2 711,031 + Pf1N1B4_614 0.89 +1.2 711,031 + Pf1N1B4_614 0.89 +0.0 711,123 - -1.2 711,123 - +1.0 711,214 + +0.2 711,237 + +0.1 711,756 + Pf1N1B4_615 0.43 -0.6 711,757 - Pf1N1B4_615 0.43 -1.8 712,344 + +0.8 712,344 + Pf1N1B4_615 0.90 -0.5 712,345 - Pf1N1B4_615 0.90 -0.4 712,345 - Pf1N1B4_615 0.90 -1.5 712,345 - Pf1N1B4_615 0.90 +0.5 712,467 + +0.3 712,468 - -1.2 712,608 + -0.1 712,609 - -0.1 712,647 + Pf1N1B4_616 0.10 +1.4 712,648 - Pf1N1B4_616 0.10 -0.2 712,648 - Pf1N1B4_616 0.10 -0.2 712,770 + Pf1N1B4_616 0.23 +0.8 712,770 + Pf1N1B4_616 0.23 +0.5 712,771 - Pf1N1B4_616 0.23 -0.4 712,771 - Pf1N1B4_616 0.23 -1.2
Or see this region's nucleotide sequence