Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_6029 and Pf1N1B4_6030 are separated by 33 nucleotides Pf1N1B4_6030 and Pf1N1B4_6031 overlap by 4 nucleotides Pf1N1B4_6031 and Pf1N1B4_6032 are separated by 56 nucleotides
Pf1N1B4_6029 - uridine nucleosidase (EC 3.2.2.3) <small>(from data)</small>, at 493,499 to 494,527
_6029
Pf1N1B4_6030 - Ribose ABC transport system, high affinity permease RbsD (TC 3.A.1.2.1), at 494,561 to 494,965
_6030
Pf1N1B4_6031 - Ribokinase (EC 2.7.1.15) <small>(from data)</small>, at 494,962 to 495,879
_6031
Pf1N1B4_6032 - Ribose operon repressor, at 495,936 to 496,955
_6032
Position (kb) 494
495 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 493.600 kb on - strand at 493.600 kb on - strand at 493.652 kb on + strand, within Pf1N1B4_6029 at 493.652 kb on + strand, within Pf1N1B4_6029 at 493.653 kb on - strand at 493.696 kb on - strand, within Pf1N1B4_6029 at 493.709 kb on - strand, within Pf1N1B4_6029 at 493.764 kb on + strand, within Pf1N1B4_6029 at 493.765 kb on - strand, within Pf1N1B4_6029 at 493.765 kb on - strand, within Pf1N1B4_6029 at 493.765 kb on - strand, within Pf1N1B4_6029 at 493.875 kb on + strand, within Pf1N1B4_6029 at 493.876 kb on - strand, within Pf1N1B4_6029 at 494.118 kb on + strand, within Pf1N1B4_6029 at 494.119 kb on - strand, within Pf1N1B4_6029 at 494.119 kb on - strand, within Pf1N1B4_6029 at 494.209 kb on - strand, within Pf1N1B4_6029 at 494.209 kb on - strand at 494.209 kb on - strand, within Pf1N1B4_6029 at 494.351 kb on - strand, within Pf1N1B4_6029 at 494.517 kb on + strand at 494.518 kb on - strand at 494.518 kb on - strand at 494.563 kb on - strand at 494.563 kb on - strand at 494.563 kb on - strand at 494.571 kb on - strand at 494.571 kb on - strand at 494.599 kb on - strand at 494.792 kb on + strand, within Pf1N1B4_6030 at 494.792 kb on + strand, within Pf1N1B4_6030 at 494.792 kb on + strand, within Pf1N1B4_6030 at 494.792 kb on + strand, within Pf1N1B4_6030 at 494.793 kb on - strand, within Pf1N1B4_6030 at 494.793 kb on - strand, within Pf1N1B4_6030 at 494.806 kb on + strand, within Pf1N1B4_6030 at 494.807 kb on - strand, within Pf1N1B4_6030 at 494.807 kb on - strand, within Pf1N1B4_6030 at 494.974 kb on - strand at 494.983 kb on + strand at 494.983 kb on + strand at 494.983 kb on + strand at 494.984 kb on - strand at 494.984 kb on - strand at 494.984 kb on - strand at 495.493 kb on + strand, within Pf1N1B4_6031 at 495.494 kb on - strand, within Pf1N1B4_6031 at 495.681 kb on - strand, within Pf1N1B4_6031 at 495.857 kb on - strand at 495.857 kb on - strand at 495.857 kb on - strand at 495.860 kb on - strand at 495.879 kb on + strand at 495.879 kb on + strand at 495.879 kb on + strand at 495.879 kb on + strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.927 kb on - strand at 495.927 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 493,600 - -0.6 493,600 - -0.1 493,652 + Pf1N1B4_6029 0.15 +0.2 493,652 + Pf1N1B4_6029 0.15 -1.6 493,653 - +2.0 493,696 - Pf1N1B4_6029 0.19 -0.4 493,709 - Pf1N1B4_6029 0.20 +0.6 493,764 + Pf1N1B4_6029 0.26 +0.4 493,765 - Pf1N1B4_6029 0.26 +0.3 493,765 - Pf1N1B4_6029 0.26 +0.8 493,765 - Pf1N1B4_6029 0.26 -0.4 493,875 + Pf1N1B4_6029 0.37 +0.8 493,876 - Pf1N1B4_6029 0.37 -2.1 494,118 + Pf1N1B4_6029 0.60 +1.3 494,119 - Pf1N1B4_6029 0.60 +1.8 494,119 - Pf1N1B4_6029 0.60 -1.1 494,209 - Pf1N1B4_6029 0.69 +0.4 494,209 - +2.4 494,209 - Pf1N1B4_6029 0.69 +1.3 494,351 - Pf1N1B4_6029 0.83 +0.7 494,517 + -1.1 494,518 - +0.0 494,518 - -1.0 494,563 - +0.3 494,563 - -0.6 494,563 - -0.2 494,571 - -0.6 494,571 - +1.7 494,599 - -2.9 494,792 + Pf1N1B4_6030 0.57 +2.0 494,792 + Pf1N1B4_6030 0.57 -1.1 494,792 + Pf1N1B4_6030 0.57 -1.4 494,792 + Pf1N1B4_6030 0.57 +2.0 494,793 - Pf1N1B4_6030 0.57 -1.4 494,793 - Pf1N1B4_6030 0.57 +1.3 494,806 + Pf1N1B4_6030 0.60 +0.1 494,807 - Pf1N1B4_6030 0.61 -1.9 494,807 - Pf1N1B4_6030 0.61 -1.4 494,974 - -0.3 494,983 + -2.9 494,983 + -1.6 494,983 + +0.4 494,984 - -1.4 494,984 - -1.0 494,984 - -1.6 495,493 + Pf1N1B4_6031 0.58 -0.3 495,494 - Pf1N1B4_6031 0.58 +0.0 495,681 - Pf1N1B4_6031 0.78 +0.2 495,857 - +0.4 495,857 - +0.1 495,857 - -0.3 495,860 - -1.1 495,879 + -1.4 495,879 + -0.6 495,879 + +1.3 495,879 + +0.9 495,880 - +0.9 495,880 - -1.7 495,880 - -0.6 495,880 - +0.1 495,880 - -1.3 495,880 - +0.7 495,880 - -0.4 495,880 - +0.7 495,880 - -0.6 495,880 - -0.4 495,880 - +0.4 495,880 - +1.3 495,880 - +0.2 495,880 - +0.1 495,880 - -1.1 495,880 - +0.7 495,880 - +0.4 495,880 - -0.7 495,880 - +0.4 495,880 - -1.0 495,927 - -0.1 495,927 - -0.6
Or see this region's nucleotide sequence