Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5965 and Pf1N1B4_5966 overlap by 8 nucleotides Pf1N1B4_5966 and Pf1N1B4_5967 overlap by 4 nucleotides Pf1N1B4_5967 and Pf1N1B4_5968 are separated by 71 nucleotides
Pf1N1B4_5965 - D-amino-acid oxidase (EC 1.4.3.3), at 413,298 to 414,398
_5965
Pf1N1B4_5966 - Hypothetical, similar to sarcosine oxidase alpha subunit, 2Fe-2S domain, at 414,391 to 414,627
_5966
Pf1N1B4_5967 - Putative oxidoreductase in 4-hydroxyproline catabolic gene cluster, at 414,624 to 415,880
_5967
Pf1N1B4_5968 - Transcriptional regulator, AraC family, at 415,952 to 416,701
_5968
Position (kb) 414
415
416 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 413.894 kb on - strand, within Pf1N1B4_5965 at 413.958 kb on - strand, within Pf1N1B4_5965 at 413.976 kb on + strand, within Pf1N1B4_5965 at 413.977 kb on - strand at 414.023 kb on + strand, within Pf1N1B4_5965 at 414.138 kb on + strand, within Pf1N1B4_5965 at 414.138 kb on + strand, within Pf1N1B4_5965 at 414.139 kb on - strand, within Pf1N1B4_5965 at 414.391 kb on - strand at 414.918 kb on + strand, within Pf1N1B4_5967 at 414.919 kb on - strand, within Pf1N1B4_5967 at 414.955 kb on + strand, within Pf1N1B4_5967 at 414.956 kb on - strand, within Pf1N1B4_5967 at 414.956 kb on - strand, within Pf1N1B4_5967 at 414.956 kb on - strand, within Pf1N1B4_5967 at 415.558 kb on + strand, within Pf1N1B4_5967 at 415.558 kb on + strand, within Pf1N1B4_5967 at 415.955 kb on + strand at 415.956 kb on - strand at 415.956 kb on - strand at 416.258 kb on + strand, within Pf1N1B4_5968 at 416.259 kb on - strand, within Pf1N1B4_5968 at 416.259 kb on - strand, within Pf1N1B4_5968 at 416.619 kb on - strand, within Pf1N1B4_5968 at 416.830 kb on + strand at 416.831 kb on - strand at 416.861 kb on - strand at 416.861 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 413,894 - Pf1N1B4_5965 0.54 +0.9 413,958 - Pf1N1B4_5965 0.60 -0.0 413,976 + Pf1N1B4_5965 0.62 -0.6 413,977 - -1.1 414,023 + Pf1N1B4_5965 0.66 +0.4 414,138 + Pf1N1B4_5965 0.76 -0.0 414,138 + Pf1N1B4_5965 0.76 +0.6 414,139 - Pf1N1B4_5965 0.76 -1.1 414,391 - -0.4 414,918 + Pf1N1B4_5967 0.23 +0.4 414,919 - Pf1N1B4_5967 0.23 -1.3 414,955 + Pf1N1B4_5967 0.26 +3.3 414,956 - Pf1N1B4_5967 0.26 -1.7 414,956 - Pf1N1B4_5967 0.26 -0.2 414,956 - Pf1N1B4_5967 0.26 -1.1 415,558 + Pf1N1B4_5967 0.74 -2.1 415,558 + Pf1N1B4_5967 0.74 +0.5 415,955 + +0.2 415,956 - +0.6 415,956 - +1.3 416,258 + Pf1N1B4_5968 0.41 +0.3 416,259 - Pf1N1B4_5968 0.41 -1.1 416,259 - Pf1N1B4_5968 0.41 +0.0 416,619 - Pf1N1B4_5968 0.89 +0.2 416,830 + -1.9 416,831 - +0.4 416,861 - +1.0 416,861 - +0.3
Or see this region's nucleotide sequence