Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5961 and Pf1N1B4_5962 overlap by 42 nucleotides Pf1N1B4_5962 and Pf1N1B4_5963 are separated by 233 nucleotides
Pf1N1B4_5961 - Major facilitator superfamily transporter, at 409,546 to 411,222
_5961
Pf1N1B4_5962 - hypothetical protein, at 411,181 to 411,342
_5962
Pf1N1B4_5963 - Predicted regulator PutR for proline utilization, GntR family, at 411,576 to 412,247
_5963
Position (kb) 411
412 Strain fitness (log2 ratio) -2
-1
0
1
2 at 410.201 kb on + strand, within Pf1N1B4_5961 at 410.232 kb on - strand, within Pf1N1B4_5961 at 410.248 kb on + strand, within Pf1N1B4_5961 at 410.249 kb on - strand, within Pf1N1B4_5961 at 410.405 kb on + strand, within Pf1N1B4_5961 at 410.420 kb on + strand, within Pf1N1B4_5961 at 410.470 kb on + strand, within Pf1N1B4_5961 at 410.582 kb on + strand at 410.777 kb on + strand, within Pf1N1B4_5961 at 410.777 kb on + strand, within Pf1N1B4_5961 at 410.778 kb on - strand at 410.778 kb on - strand, within Pf1N1B4_5961 at 410.778 kb on - strand, within Pf1N1B4_5961 at 410.778 kb on - strand, within Pf1N1B4_5961 at 410.778 kb on - strand, within Pf1N1B4_5961 at 410.937 kb on + strand, within Pf1N1B4_5961 at 410.937 kb on + strand, within Pf1N1B4_5961 at 410.937 kb on + strand, within Pf1N1B4_5961 at 410.938 kb on - strand at 410.938 kb on - strand, within Pf1N1B4_5961 at 410.938 kb on - strand at 410.938 kb on - strand, within Pf1N1B4_5961 at 410.938 kb on - strand, within Pf1N1B4_5961 at 410.938 kb on - strand, within Pf1N1B4_5961 at 410.996 kb on - strand, within Pf1N1B4_5961 at 410.996 kb on - strand, within Pf1N1B4_5961 at 411.205 kb on - strand at 411.248 kb on - strand, within Pf1N1B4_5962 at 411.250 kb on - strand, within Pf1N1B4_5962 at 411.250 kb on - strand, within Pf1N1B4_5962 at 411.250 kb on - strand, within Pf1N1B4_5962 at 411.250 kb on - strand, within Pf1N1B4_5962 at 411.268 kb on - strand, within Pf1N1B4_5962 at 411.394 kb on + strand at 411.435 kb on - strand at 411.490 kb on - strand at 411.490 kb on - strand at 411.614 kb on + strand at 411.614 kb on + strand at 411.877 kb on + strand, within Pf1N1B4_5963 at 411.878 kb on - strand, within Pf1N1B4_5963 at 412.062 kb on - strand, within Pf1N1B4_5963 at 412.281 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 410,201 + Pf1N1B4_5961 0.39 -1.1 410,232 - Pf1N1B4_5961 0.41 +0.1 410,248 + Pf1N1B4_5961 0.42 +0.8 410,249 - Pf1N1B4_5961 0.42 -0.8 410,405 + Pf1N1B4_5961 0.51 -1.6 410,420 + Pf1N1B4_5961 0.52 -1.1 410,470 + Pf1N1B4_5961 0.55 +1.2 410,582 + +0.2 410,777 + Pf1N1B4_5961 0.73 -1.6 410,777 + Pf1N1B4_5961 0.73 -1.9 410,778 - -0.3 410,778 - Pf1N1B4_5961 0.73 -1.1 410,778 - Pf1N1B4_5961 0.73 -1.7 410,778 - Pf1N1B4_5961 0.73 +0.3 410,778 - Pf1N1B4_5961 0.73 -0.2 410,937 + Pf1N1B4_5961 0.83 +0.4 410,937 + Pf1N1B4_5961 0.83 +0.4 410,937 + Pf1N1B4_5961 0.83 +0.8 410,938 - +0.9 410,938 - Pf1N1B4_5961 0.83 -0.1 410,938 - -0.9 410,938 - Pf1N1B4_5961 0.83 +1.3 410,938 - Pf1N1B4_5961 0.83 -1.9 410,938 - Pf1N1B4_5961 0.83 -2.1 410,996 - Pf1N1B4_5961 0.86 +0.4 410,996 - Pf1N1B4_5961 0.86 +1.6 411,205 - +0.8 411,248 - Pf1N1B4_5962 0.41 -0.5 411,250 - Pf1N1B4_5962 0.43 -1.0 411,250 - Pf1N1B4_5962 0.43 -1.1 411,250 - Pf1N1B4_5962 0.43 -0.1 411,250 - Pf1N1B4_5962 0.43 -1.7 411,268 - Pf1N1B4_5962 0.54 +2.0 411,394 + -0.9 411,435 - +0.4 411,490 - +0.1 411,490 - -1.1 411,614 + -1.1 411,614 + -0.2 411,877 + Pf1N1B4_5963 0.45 +0.3 411,878 - Pf1N1B4_5963 0.45 -0.6 412,062 - Pf1N1B4_5963 0.72 -0.6 412,281 - +0.4
Or see this region's nucleotide sequence