Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5837 and Pf1N1B4_5838 are separated by 186 nucleotides Pf1N1B4_5838 and Pf1N1B4_5839 are separated by 170 nucleotides
Pf1N1B4_5837 - Transcriptional regulator, LysR family, at 271,285 to 272,181
_5837
Pf1N1B4_5838 - FIG00956589: hypothetical protein, at 272,368 to 273,165
_5838
Pf1N1B4_5839 - Transcriptional regulator, AraC family, at 273,336 to 274,112
_5839
Position (kb) 272
273
274 Strain fitness (log2 ratio) -2
-1
0
1
2 at 271.428 kb on - strand, within Pf1N1B4_5837 at 271.604 kb on + strand, within Pf1N1B4_5837 at 271.604 kb on + strand, within Pf1N1B4_5837 at 271.604 kb on + strand at 271.604 kb on + strand, within Pf1N1B4_5837 at 271.604 kb on + strand, within Pf1N1B4_5837 at 271.604 kb on + strand, within Pf1N1B4_5837 at 271.605 kb on - strand, within Pf1N1B4_5837 at 271.605 kb on - strand, within Pf1N1B4_5837 at 271.605 kb on - strand, within Pf1N1B4_5837 at 271.605 kb on - strand, within Pf1N1B4_5837 at 271.699 kb on + strand, within Pf1N1B4_5837 at 271.699 kb on + strand, within Pf1N1B4_5837 at 271.795 kb on + strand, within Pf1N1B4_5837 at 271.796 kb on - strand, within Pf1N1B4_5837 at 272.101 kb on + strand at 272.101 kb on + strand at 272.101 kb on + strand at 272.101 kb on + strand at 272.213 kb on + strand at 272.213 kb on + strand at 272.214 kb on - strand at 272.217 kb on + strand at 272.217 kb on + strand at 272.217 kb on + strand at 272.218 kb on - strand at 272.218 kb on - strand at 272.227 kb on + strand at 272.233 kb on - strand at 272.279 kb on + strand at 272.279 kb on + strand at 272.279 kb on + strand at 272.279 kb on + strand at 272.279 kb on + strand at 272.280 kb on - strand at 272.280 kb on - strand at 272.280 kb on - strand at 272.280 kb on - strand at 272.280 kb on - strand at 272.390 kb on + strand at 272.390 kb on + strand at 272.726 kb on + strand, within Pf1N1B4_5838 at 272.727 kb on - strand, within Pf1N1B4_5838 at 272.903 kb on + strand, within Pf1N1B4_5838 at 272.989 kb on + strand, within Pf1N1B4_5838 at 272.990 kb on - strand, within Pf1N1B4_5838 at 273.184 kb on + strand at 273.381 kb on + strand at 273.549 kb on - strand, within Pf1N1B4_5839 at 273.676 kb on - strand, within Pf1N1B4_5839
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 271,428 - Pf1N1B4_5837 0.16 +1.0 271,604 + Pf1N1B4_5837 0.36 -0.3 271,604 + Pf1N1B4_5837 0.36 -0.8 271,604 + -0.1 271,604 + Pf1N1B4_5837 0.36 +0.8 271,604 + Pf1N1B4_5837 0.36 +0.9 271,604 + Pf1N1B4_5837 0.36 +1.3 271,605 - Pf1N1B4_5837 0.36 +0.3 271,605 - Pf1N1B4_5837 0.36 +0.1 271,605 - Pf1N1B4_5837 0.36 -0.4 271,605 - Pf1N1B4_5837 0.36 -0.8 271,699 + Pf1N1B4_5837 0.46 -1.2 271,699 + Pf1N1B4_5837 0.46 -0.0 271,795 + Pf1N1B4_5837 0.57 -0.3 271,796 - Pf1N1B4_5837 0.57 -1.6 272,101 + +0.4 272,101 + +0.3 272,101 + +1.4 272,101 + -0.6 272,213 + -2.6 272,213 + -0.6 272,214 - +1.0 272,217 + -0.6 272,217 + -0.6 272,217 + -1.0 272,218 - -0.8 272,218 - +2.0 272,227 + -0.9 272,233 - +1.0 272,279 + -0.4 272,279 + -0.9 272,279 + +0.1 272,279 + -1.1 272,279 + +0.4 272,280 - -0.7 272,280 - -1.6 272,280 - +2.3 272,280 - +0.7 272,280 - +2.0 272,390 + -0.1 272,390 + +0.4 272,726 + Pf1N1B4_5838 0.45 +0.1 272,727 - Pf1N1B4_5838 0.45 +0.4 272,903 + Pf1N1B4_5838 0.67 -0.8 272,989 + Pf1N1B4_5838 0.78 +0.1 272,990 - Pf1N1B4_5838 0.78 -1.9 273,184 + +1.0 273,381 + -1.9 273,549 - Pf1N1B4_5839 0.27 +0.4 273,676 - Pf1N1B4_5839 0.44 +2.0
Or see this region's nucleotide sequence