Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5833 and Pf1N1B4_5834 are separated by 36 nucleotides Pf1N1B4_5834 and Pf1N1B4_5835 are separated by 11 nucleotides Pf1N1B4_5835 and Pf1N1B4_5836 are separated by 133 nucleotides Pf1N1B4_5836 and Pf1N1B4_5837 are separated by 110 nucleotides
Pf1N1B4_5833 - Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit A (EC 2.8.3.5), at 267,845 to 268,543
_5833
Pf1N1B4_5834 - Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit B (EC 2.8.3.5), at 268,580 to 269,245
_5834
Pf1N1B4_5835 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 269,257 to 270,438
_5835
Pf1N1B4_5836 - Putative polyketide synthase (Fragment), at 270,572 to 271,174
_5836
Pf1N1B4_5837 - Transcriptional regulator, LysR family, at 271,285 to 272,181
_5837
Position (kb) 269
270
271 Strain fitness (log2 ratio) -1
0
1
2 at 268.275 kb on - strand, within Pf1N1B4_5833 at 268.478 kb on + strand at 268.478 kb on + strand at 268.478 kb on + strand at 268.478 kb on + strand at 268.479 kb on - strand at 268.479 kb on - strand at 268.572 kb on + strand at 268.640 kb on + strand at 268.640 kb on + strand at 268.640 kb on + strand at 268.641 kb on - strand at 268.682 kb on + strand, within Pf1N1B4_5834 at 268.682 kb on + strand, within Pf1N1B4_5834 at 268.683 kb on - strand, within Pf1N1B4_5834 at 268.683 kb on - strand, within Pf1N1B4_5834 at 268.683 kb on - strand, within Pf1N1B4_5834 at 269.423 kb on + strand, within Pf1N1B4_5835 at 269.623 kb on + strand, within Pf1N1B4_5835 at 269.624 kb on - strand, within Pf1N1B4_5835 at 269.624 kb on - strand, within Pf1N1B4_5835 at 269.624 kb on - strand, within Pf1N1B4_5835 at 269.758 kb on + strand, within Pf1N1B4_5835 at 269.759 kb on - strand, within Pf1N1B4_5835 at 269.759 kb on - strand, within Pf1N1B4_5835 at 270.436 kb on + strand at 270.553 kb on + strand at 270.554 kb on - strand at 271.049 kb on + strand, within Pf1N1B4_5836 at 271.049 kb on + strand, within Pf1N1B4_5836 at 271.050 kb on - strand, within Pf1N1B4_5836 at 271.182 kb on - strand at 271.209 kb on - strand at 271.267 kb on + strand at 271.267 kb on + strand at 271.270 kb on + strand at 271.271 kb on - strand at 271.428 kb on - strand, within Pf1N1B4_5837
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 268,275 - Pf1N1B4_5833 0.62 +0.4 268,478 + +0.1 268,478 + +0.4 268,478 + -0.3 268,478 + +1.1 268,479 - -1.4 268,479 - -0.1 268,572 + +1.4 268,640 + -0.6 268,640 + -0.9 268,640 + +0.5 268,641 - +0.4 268,682 + Pf1N1B4_5834 0.15 -0.6 268,682 + Pf1N1B4_5834 0.15 +1.3 268,683 - Pf1N1B4_5834 0.15 +1.4 268,683 - Pf1N1B4_5834 0.15 +0.4 268,683 - Pf1N1B4_5834 0.15 -0.1 269,423 + Pf1N1B4_5835 0.14 -0.6 269,623 + Pf1N1B4_5835 0.31 -1.0 269,624 - Pf1N1B4_5835 0.31 +0.2 269,624 - Pf1N1B4_5835 0.31 -0.6 269,624 - Pf1N1B4_5835 0.31 -1.4 269,758 + Pf1N1B4_5835 0.42 -0.6 269,759 - Pf1N1B4_5835 0.42 -0.2 269,759 - Pf1N1B4_5835 0.42 +0.4 270,436 + +1.4 270,553 + -0.9 270,554 - +1.6 271,049 + Pf1N1B4_5836 0.79 -0.2 271,049 + Pf1N1B4_5836 0.79 +2.0 271,050 - Pf1N1B4_5836 0.79 -0.3 271,182 - -0.4 271,209 - +0.4 271,267 + -0.1 271,267 + -1.3 271,270 + +1.0 271,271 - +0.6 271,428 - Pf1N1B4_5837 0.16 +1.0
Or see this region's nucleotide sequence