Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_58

Experiment: Spectinomycin 0.025 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_57 and Pf1N1B4_58 are separated by 178 nucleotidesPf1N1B4_58 and Pf1N1B4_59 are separated by 244 nucleotidesPf1N1B4_59 and Pf1N1B4_60 overlap by 1 nucleotides Pf1N1B4_57 - tRNA dihydrouridine synthase A, at 61,248 to 62,186 _57 Pf1N1B4_58 - Transaldolase (EC 2.2.1.2), at 62,365 to 63,291 _58 Pf1N1B4_59 - Serine-protein kinase RsbW (EC 2.7.11.1), at 63,536 to 64,018 _59 Pf1N1B4_60 - Serine phosphatase RsbU, regulator of sigma subunit, at 64,018 to 65,199 _60 Position (kb) 62 63 64Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 61.520 kb on + strand, within Pf1N1B4_57at 61.520 kb on + strandat 61.520 kb on + strand, within Pf1N1B4_57at 61.520 kb on + strand, within Pf1N1B4_57at 61.520 kb on + strand, within Pf1N1B4_57at 61.521 kb on - strand, within Pf1N1B4_57at 61.521 kb on - strand, within Pf1N1B4_57at 61.521 kb on - strand, within Pf1N1B4_57at 61.628 kb on + strand, within Pf1N1B4_57at 61.628 kb on + strand, within Pf1N1B4_57at 61.644 kb on + strand, within Pf1N1B4_57at 61.700 kb on + strand, within Pf1N1B4_57at 61.700 kb on + strand, within Pf1N1B4_57at 61.700 kb on + strand, within Pf1N1B4_57at 61.701 kb on - strand, within Pf1N1B4_57at 61.820 kb on + strand, within Pf1N1B4_57at 61.821 kb on - strand, within Pf1N1B4_57at 61.917 kb on + strand, within Pf1N1B4_57at 61.917 kb on + strand, within Pf1N1B4_57at 61.918 kb on - strandat 62.004 kb on + strand, within Pf1N1B4_57at 62.004 kb on + strand, within Pf1N1B4_57at 62.004 kb on + strand, within Pf1N1B4_57at 62.004 kb on + strand, within Pf1N1B4_57at 62.004 kb on + strand, within Pf1N1B4_57at 62.004 kb on + strand, within Pf1N1B4_57at 62.004 kb on + strandat 62.006 kb on + strandat 62.006 kb on + strand, within Pf1N1B4_57at 62.006 kb on + strand, within Pf1N1B4_57at 62.006 kb on + strand, within Pf1N1B4_57at 62.006 kb on + strand, within Pf1N1B4_57at 62.046 kb on - strand, within Pf1N1B4_57at 62.126 kb on + strandat 62.226 kb on + strandat 62.226 kb on + strandat 62.226 kb on + strandat 62.226 kb on + strandat 62.226 kb on + strandat 62.226 kb on + strandat 62.226 kb on + strandat 62.226 kb on + strandat 62.227 kb on - strandat 62.293 kb on + strandat 62.299 kb on + strandat 62.299 kb on + strandat 62.332 kb on - strandat 62.340 kb on + strandat 62.340 kb on + strandat 62.341 kb on - strandat 62.341 kb on - strandat 62.341 kb on - strandat 62.609 kb on + strand, within Pf1N1B4_58at 62.619 kb on + strandat 62.619 kb on + strand, within Pf1N1B4_58at 62.619 kb on + strand, within Pf1N1B4_58at 62.620 kb on - strand, within Pf1N1B4_58at 62.667 kb on - strand, within Pf1N1B4_58at 62.890 kb on + strand, within Pf1N1B4_58at 62.923 kb on + strandat 62.923 kb on + strand, within Pf1N1B4_58at 62.968 kb on + strand, within Pf1N1B4_58at 62.968 kb on + strand, within Pf1N1B4_58at 62.968 kb on + strand, within Pf1N1B4_58at 62.968 kb on + strand, within Pf1N1B4_58at 62.968 kb on + strand, within Pf1N1B4_58at 62.968 kb on + strand, within Pf1N1B4_58at 62.968 kb on + strand, within Pf1N1B4_58at 62.969 kb on - strand, within Pf1N1B4_58at 62.974 kb on + strand, within Pf1N1B4_58at 62.974 kb on + strand, within Pf1N1B4_58at 62.974 kb on + strand, within Pf1N1B4_58at 62.974 kb on + strand, within Pf1N1B4_58at 62.974 kb on + strand, within Pf1N1B4_58at 62.974 kb on + strand, within Pf1N1B4_58at 62.977 kb on + strand, within Pf1N1B4_58at 62.977 kb on + strandat 62.977 kb on + strand, within Pf1N1B4_58at 62.977 kb on + strand, within Pf1N1B4_58at 62.977 kb on + strand, within Pf1N1B4_58at 62.977 kb on + strand, within Pf1N1B4_58at 62.977 kb on + strand, within Pf1N1B4_58at 62.977 kb on + strandat 62.992 kb on + strand, within Pf1N1B4_58at 62.992 kb on + strand, within Pf1N1B4_58at 63.294 kb on + strandat 63.367 kb on + strandat 63.367 kb on + strandat 63.440 kb on + strandat 63.440 kb on + strandat 63.441 kb on - strandat 63.445 kb on + strandat 63.445 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml
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61,520 + Pf1N1B4_57 0.29 +1.6
61,520 + +0.0
61,520 + Pf1N1B4_57 0.29 -1.1
61,520 + Pf1N1B4_57 0.29 -1.0
61,520 + Pf1N1B4_57 0.29 -1.0
61,521 - Pf1N1B4_57 0.29 +0.9
61,521 - Pf1N1B4_57 0.29 +0.1
61,521 - Pf1N1B4_57 0.29 -1.0
61,628 + Pf1N1B4_57 0.40 -0.7
61,628 + Pf1N1B4_57 0.40 -0.2
61,644 + Pf1N1B4_57 0.42 +1.0
61,700 + Pf1N1B4_57 0.48 +0.0
61,700 + Pf1N1B4_57 0.48 -1.4
61,700 + Pf1N1B4_57 0.48 -0.6
61,701 - Pf1N1B4_57 0.48 -0.3
61,820 + Pf1N1B4_57 0.61 -1.4
61,821 - Pf1N1B4_57 0.61 -1.0
61,917 + Pf1N1B4_57 0.71 +0.6
61,917 + Pf1N1B4_57 0.71 +0.0
61,918 - +0.6
62,004 + Pf1N1B4_57 0.81 -0.2
62,004 + Pf1N1B4_57 0.81 -0.1
62,004 + Pf1N1B4_57 0.81 +0.9
62,004 + Pf1N1B4_57 0.81 +1.4
62,004 + Pf1N1B4_57 0.81 +1.0
62,004 + Pf1N1B4_57 0.81 -1.2
62,004 + +0.6
62,006 + +0.2
62,006 + Pf1N1B4_57 0.81 +0.6
62,006 + Pf1N1B4_57 0.81 -0.1
62,006 + Pf1N1B4_57 0.81 -0.1
62,006 + Pf1N1B4_57 0.81 +0.6
62,046 - Pf1N1B4_57 0.85 -1.7
62,126 + -0.4
62,226 + +0.6
62,226 + -1.0
62,226 + +1.3
62,226 + -2.0
62,226 + -0.3
62,226 + +0.1
62,226 + -0.8
62,226 + +0.3
62,227 - -0.2
62,293 + -0.4
62,299 + +0.6
62,299 + +0.8
62,332 - +0.6
62,340 + +0.6
62,340 + +0.8
62,341 - -1.4
62,341 - -2.2
62,341 - -0.4
62,609 + Pf1N1B4_58 0.26 -2.0
62,619 + -2.0
62,619 + Pf1N1B4_58 0.27 +0.6
62,619 + Pf1N1B4_58 0.27 -0.6
62,620 - Pf1N1B4_58 0.28 -2.2
62,667 - Pf1N1B4_58 0.33 -2.2
62,890 + Pf1N1B4_58 0.57 -1.0
62,923 + +0.0
62,923 + Pf1N1B4_58 0.60 -2.7
62,968 + Pf1N1B4_58 0.65 -0.0
62,968 + Pf1N1B4_58 0.65 -0.8
62,968 + Pf1N1B4_58 0.65 -1.4
62,968 + Pf1N1B4_58 0.65 -1.4
62,968 + Pf1N1B4_58 0.65 -1.7
62,968 + Pf1N1B4_58 0.65 +0.6
62,968 + Pf1N1B4_58 0.65 -0.1
62,969 - Pf1N1B4_58 0.65 -2.2
62,974 + Pf1N1B4_58 0.66 -0.4
62,974 + Pf1N1B4_58 0.66 +0.0
62,974 + Pf1N1B4_58 0.66 -0.8
62,974 + Pf1N1B4_58 0.66 -0.2
62,974 + Pf1N1B4_58 0.66 -0.2
62,974 + Pf1N1B4_58 0.66 -1.4
62,977 + Pf1N1B4_58 0.66 -2.2
62,977 + -1.4
62,977 + Pf1N1B4_58 0.66 -2.8
62,977 + Pf1N1B4_58 0.66 +2.4
62,977 + Pf1N1B4_58 0.66 +0.1
62,977 + Pf1N1B4_58 0.66 -0.3
62,977 + Pf1N1B4_58 0.66 -0.4
62,977 + -1.0
62,992 + Pf1N1B4_58 0.68 -0.7
62,992 + Pf1N1B4_58 0.68 -1.7
63,294 + +0.2
63,367 + -1.1
63,367 + -0.6
63,440 + +0.9
63,440 + +0.1
63,441 - +0.4
63,445 + -0.1
63,445 + +0.6

Or see this region's nucleotide sequence