Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5714

Experiment: Spectinomycin 0.025 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5712 and Pf1N1B4_5713 are separated by 149 nucleotidesPf1N1B4_5713 and Pf1N1B4_5714 are separated by 27 nucleotidesPf1N1B4_5714 and Pf1N1B4_5715 are separated by 108 nucleotides Pf1N1B4_5712 - Adenylate cyclase (EC 4.6.1.1) / Guanylate cyclase (EC 4.6.1.2), at 151,717 to 154,614 _5712 Pf1N1B4_5713 - hypothetical protein, at 154,764 to 154,979 _5713 Pf1N1B4_5714 - RND efflux system, outer membrane lipoprotein, NodT family, at 155,007 to 156,611 _5714 Pf1N1B4_5715 - RND efflux membrane fusion protein, at 156,720 to 157,754 _5715 Position (kb) 155 156 157Strain fitness (log2 ratio) -1 0 1 2 3at 154.292 kb on + strand, within Pf1N1B4_5712at 154.572 kb on + strandat 154.573 kb on - strandat 154.594 kb on - strandat 154.594 kb on - strandat 154.807 kb on - strand, within Pf1N1B4_5713at 154.807 kb on - strand, within Pf1N1B4_5713at 155.080 kb on + strandat 155.081 kb on - strandat 155.500 kb on - strand, within Pf1N1B4_5714at 155.689 kb on - strand, within Pf1N1B4_5714at 156.019 kb on + strand, within Pf1N1B4_5714at 156.522 kb on + strandat 156.522 kb on + strandat 156.523 kb on - strandat 156.523 kb on - strandat 156.523 kb on - strandat 156.523 kb on - strandat 156.523 kb on - strandat 156.799 kb on + strandat 156.868 kb on + strand, within Pf1N1B4_5715at 157.061 kb on + strand, within Pf1N1B4_5715at 157.062 kb on - strand, within Pf1N1B4_5715at 157.062 kb on - strand, within Pf1N1B4_5715at 157.062 kb on - strand, within Pf1N1B4_5715at 157.062 kb on - strand, within Pf1N1B4_5715at 157.137 kb on + strand, within Pf1N1B4_5715at 157.137 kb on + strand, within Pf1N1B4_5715at 157.138 kb on - strand, within Pf1N1B4_5715at 157.138 kb on - strandat 157.396 kb on - strand, within Pf1N1B4_5715

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Per-strain Table

Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml
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154,292 + Pf1N1B4_5712 0.89 +0.1
154,572 + +0.4
154,573 - +0.8
154,594 - -1.0
154,594 - -0.3
154,807 - Pf1N1B4_5713 0.20 +1.7
154,807 - Pf1N1B4_5713 0.20 -0.9
155,080 + +1.7
155,081 - +1.4
155,500 - Pf1N1B4_5714 0.31 +1.4
155,689 - Pf1N1B4_5714 0.42 +3.0
156,019 + Pf1N1B4_5714 0.63 +1.2
156,522 + +1.8
156,522 + +2.4
156,523 - +0.1
156,523 - +1.2
156,523 - +1.4
156,523 - -0.8
156,523 - +0.4
156,799 + -0.8
156,868 + Pf1N1B4_5715 0.14 -0.0
157,061 + Pf1N1B4_5715 0.33 +0.9
157,062 - Pf1N1B4_5715 0.33 +0.1
157,062 - Pf1N1B4_5715 0.33 -0.5
157,062 - Pf1N1B4_5715 0.33 -1.4
157,062 - Pf1N1B4_5715 0.33 +0.7
157,137 + Pf1N1B4_5715 0.40 -0.2
157,137 + Pf1N1B4_5715 0.40 -0.6
157,138 - Pf1N1B4_5715 0.40 +0.1
157,138 - -0.6
157,396 - Pf1N1B4_5715 0.65 -0.6

Or see this region's nucleotide sequence