Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5712 and Pf1N1B4_5713 are separated by 149 nucleotides Pf1N1B4_5713 and Pf1N1B4_5714 are separated by 27 nucleotides Pf1N1B4_5714 and Pf1N1B4_5715 are separated by 108 nucleotides
Pf1N1B4_5712 - Adenylate cyclase (EC 4.6.1.1) / Guanylate cyclase (EC 4.6.1.2), at 151,717 to 154,614
_5712
Pf1N1B4_5713 - hypothetical protein, at 154,764 to 154,979
_5713
Pf1N1B4_5714 - RND efflux system, outer membrane lipoprotein, NodT family, at 155,007 to 156,611
_5714
Pf1N1B4_5715 - RND efflux membrane fusion protein, at 156,720 to 157,754
_5715
Position (kb)
155
156
157 Strain fitness (log2 ratio)
-1
0
1
2
3 at 154.292 kb on + strand, within Pf1N1B4_5712 at 154.572 kb on + strand at 154.573 kb on - strand at 154.594 kb on - strand at 154.594 kb on - strand at 154.807 kb on - strand, within Pf1N1B4_5713 at 154.807 kb on - strand, within Pf1N1B4_5713 at 155.080 kb on + strand at 155.081 kb on - strand at 155.500 kb on - strand, within Pf1N1B4_5714 at 155.689 kb on - strand, within Pf1N1B4_5714 at 156.019 kb on + strand, within Pf1N1B4_5714 at 156.522 kb on + strand at 156.522 kb on + strand at 156.523 kb on - strand at 156.523 kb on - strand at 156.523 kb on - strand at 156.523 kb on - strand at 156.523 kb on - strand at 156.799 kb on + strand at 156.868 kb on + strand, within Pf1N1B4_5715 at 157.061 kb on + strand, within Pf1N1B4_5715 at 157.062 kb on - strand, within Pf1N1B4_5715 at 157.062 kb on - strand, within Pf1N1B4_5715 at 157.062 kb on - strand, within Pf1N1B4_5715 at 157.062 kb on - strand, within Pf1N1B4_5715 at 157.137 kb on + strand, within Pf1N1B4_5715 at 157.137 kb on + strand, within Pf1N1B4_5715 at 157.138 kb on - strand, within Pf1N1B4_5715 at 157.138 kb on - strand at 157.396 kb on - strand, within Pf1N1B4_5715
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 154,292 + Pf1N1B4_5712 0.89 +0.1 154,572 + +0.4 154,573 - +0.8 154,594 - -1.0 154,594 - -0.3 154,807 - Pf1N1B4_5713 0.20 +1.7 154,807 - Pf1N1B4_5713 0.20 -0.9 155,080 + +1.7 155,081 - +1.4 155,500 - Pf1N1B4_5714 0.31 +1.4 155,689 - Pf1N1B4_5714 0.42 +3.0 156,019 + Pf1N1B4_5714 0.63 +1.2 156,522 + +1.8 156,522 + +2.4 156,523 - +0.1 156,523 - +1.2 156,523 - +1.4 156,523 - -0.8 156,523 - +0.4 156,799 + -0.8 156,868 + Pf1N1B4_5715 0.14 -0.0 157,061 + Pf1N1B4_5715 0.33 +0.9 157,062 - Pf1N1B4_5715 0.33 +0.1 157,062 - Pf1N1B4_5715 0.33 -0.5 157,062 - Pf1N1B4_5715 0.33 -1.4 157,062 - Pf1N1B4_5715 0.33 +0.7 157,137 + Pf1N1B4_5715 0.40 -0.2 157,137 + Pf1N1B4_5715 0.40 -0.6 157,138 - Pf1N1B4_5715 0.40 +0.1 157,138 - -0.6 157,396 - Pf1N1B4_5715 0.65 -0.6
Or see this region's nucleotide sequence