Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5498 and Pf1N1B4_5499 are separated by 367 nucleotides
Pf1N1B4_5498 - hypothetical protein, at 247,310 to 247,522
_5498
Pf1N1B4_5499 - hypothetical protein, at 247,890 to 249,695
_5499
Position (kb) 247
248 Strain fitness (log2 ratio) -2
-1
0
1
2 at 246.375 kb on + strand at 246.375 kb on + strand at 246.376 kb on - strand at 246.537 kb on - strand at 246.810 kb on + strand at 246.884 kb on - strand at 246.884 kb on - strand at 247.038 kb on + strand at 247.038 kb on + strand at 247.041 kb on - strand at 247.041 kb on - strand at 247.109 kb on + strand at 247.150 kb on - strand at 247.191 kb on + strand at 247.192 kb on - strand at 247.219 kb on + strand at 247.252 kb on + strand at 247.252 kb on + strand at 247.253 kb on - strand at 247.253 kb on - strand at 247.255 kb on - strand at 247.361 kb on + strand, within Pf1N1B4_5498 at 247.362 kb on - strand, within Pf1N1B4_5498 at 247.362 kb on - strand, within Pf1N1B4_5498 at 247.462 kb on + strand, within Pf1N1B4_5498 at 247.462 kb on + strand, within Pf1N1B4_5498 at 247.631 kb on + strand at 247.632 kb on - strand at 247.632 kb on - strand at 247.632 kb on - strand at 247.632 kb on - strand at 247.683 kb on + strand at 247.684 kb on - strand at 247.705 kb on + strand at 247.706 kb on - strand at 247.706 kb on - strand at 247.706 kb on - strand at 247.706 kb on - strand at 247.780 kb on + strand at 247.780 kb on + strand at 247.780 kb on + strand at 247.781 kb on - strand at 247.781 kb on - strand at 247.855 kb on + strand at 248.092 kb on + strand, within Pf1N1B4_5499 at 248.092 kb on + strand, within Pf1N1B4_5499 at 248.093 kb on - strand at 248.320 kb on + strand, within Pf1N1B4_5499 at 248.356 kb on + strand at 248.356 kb on + strand, within Pf1N1B4_5499 at 248.356 kb on + strand, within Pf1N1B4_5499 at 248.357 kb on - strand, within Pf1N1B4_5499 at 248.357 kb on - strand, within Pf1N1B4_5499 at 248.357 kb on - strand, within Pf1N1B4_5499 at 248.357 kb on - strand, within Pf1N1B4_5499 at 248.357 kb on - strand at 248.434 kb on + strand, within Pf1N1B4_5499 at 248.434 kb on + strand, within Pf1N1B4_5499 at 248.434 kb on + strand, within Pf1N1B4_5499 at 248.434 kb on + strand, within Pf1N1B4_5499 at 248.434 kb on + strand, within Pf1N1B4_5499 at 248.434 kb on + strand, within Pf1N1B4_5499 at 248.435 kb on - strand, within Pf1N1B4_5499 at 248.435 kb on - strand, within Pf1N1B4_5499 at 248.515 kb on + strand, within Pf1N1B4_5499 at 248.515 kb on + strand, within Pf1N1B4_5499 at 248.515 kb on + strand, within Pf1N1B4_5499 at 248.516 kb on - strand, within Pf1N1B4_5499 at 248.516 kb on - strand, within Pf1N1B4_5499
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 246,375 + +0.1 246,375 + -1.1 246,376 - +0.9 246,537 - +0.3 246,810 + +0.7 246,884 - +0.4 246,884 - -0.3 247,038 + +0.4 247,038 + -0.9 247,041 - -0.9 247,041 - -1.2 247,109 + -0.7 247,150 - -0.6 247,191 + -0.5 247,192 - -0.9 247,219 + +0.1 247,252 + -0.6 247,252 + -0.3 247,253 - -0.6 247,253 - -0.1 247,255 - +0.5 247,361 + Pf1N1B4_5498 0.24 -2.6 247,362 - Pf1N1B4_5498 0.24 +0.4 247,362 - Pf1N1B4_5498 0.24 -1.3 247,462 + Pf1N1B4_5498 0.71 +0.7 247,462 + Pf1N1B4_5498 0.71 -1.6 247,631 + +0.1 247,632 - -0.6 247,632 - -0.3 247,632 - +0.2 247,632 - +1.4 247,683 + +1.4 247,684 - +0.2 247,705 + +1.1 247,706 - +0.7 247,706 - +2.0 247,706 - -1.6 247,706 - -0.2 247,780 + +0.3 247,780 + +0.3 247,780 + -2.6 247,781 - -2.1 247,781 - -0.9 247,855 + +0.0 248,092 + Pf1N1B4_5499 0.11 -0.6 248,092 + Pf1N1B4_5499 0.11 -1.9 248,093 - +0.7 248,320 + Pf1N1B4_5499 0.24 -0.5 248,356 + -0.3 248,356 + Pf1N1B4_5499 0.26 -0.3 248,356 + Pf1N1B4_5499 0.26 -1.3 248,357 - Pf1N1B4_5499 0.26 +0.1 248,357 - Pf1N1B4_5499 0.26 -0.3 248,357 - Pf1N1B4_5499 0.26 -0.4 248,357 - Pf1N1B4_5499 0.26 -0.8 248,357 - -0.9 248,434 + Pf1N1B4_5499 0.30 +0.9 248,434 + Pf1N1B4_5499 0.30 -0.4 248,434 + Pf1N1B4_5499 0.30 +0.7 248,434 + Pf1N1B4_5499 0.30 +0.5 248,434 + Pf1N1B4_5499 0.30 -0.6 248,434 + Pf1N1B4_5499 0.30 -1.3 248,435 - Pf1N1B4_5499 0.30 +0.7 248,435 - Pf1N1B4_5499 0.30 -0.4 248,515 + Pf1N1B4_5499 0.35 -0.1 248,515 + Pf1N1B4_5499 0.35 -0.1 248,515 + Pf1N1B4_5499 0.35 -0.5 248,516 - Pf1N1B4_5499 0.35 -0.5 248,516 - Pf1N1B4_5499 0.35 -0.1
Or see this region's nucleotide sequence