Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5356 and Pf1N1B4_5357 are separated by 907 nucleotides Pf1N1B4_5357 and Pf1N1B4_5358 are separated by 540 nucleotides Pf1N1B4_5358 and Pf1N1B4_5359 are separated by 80 nucleotides
Pf1N1B4_5356 - hypothetical protein, at 92,977 to 93,222
_5356
Pf1N1B4_5357 - hypothetical protein, at 94,130 to 94,306
_5357
Pf1N1B4_5358 - hypothetical protein, at 94,847 to 95,005
_5358
Pf1N1B4_5359 - hypothetical protein, at 95,086 to 95,373
_5359
Position (kb)
94
95 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 93.147 kb on + strand, within Pf1N1B4_5356 at 93.147 kb on + strand, within Pf1N1B4_5356 at 93.185 kb on + strand at 93.186 kb on - strand, within Pf1N1B4_5356 at 93.220 kb on + strand at 93.283 kb on - strand at 93.315 kb on + strand at 93.315 kb on + strand at 93.315 kb on + strand at 93.316 kb on - strand at 93.466 kb on - strand at 93.529 kb on + strand at 93.529 kb on + strand at 93.529 kb on + strand at 93.529 kb on + strand at 93.529 kb on + strand at 93.529 kb on + strand at 93.529 kb on + strand at 93.529 kb on + strand at 93.529 kb on + strand at 93.529 kb on + strand at 93.529 kb on + strand at 93.529 kb on + strand at 93.529 kb on + strand at 93.529 kb on + strand at 93.529 kb on + strand at 93.529 kb on + strand at 93.529 kb on + strand at 93.530 kb on - strand at 93.530 kb on - strand at 93.530 kb on - strand at 93.530 kb on - strand at 93.640 kb on + strand at 93.680 kb on + strand at 93.760 kb on - strand at 93.769 kb on + strand at 93.941 kb on + strand at 93.941 kb on + strand at 93.984 kb on + strand at 93.985 kb on - strand at 93.985 kb on - strand at 94.078 kb on - strand at 94.086 kb on + strand at 94.086 kb on + strand at 94.096 kb on + strand at 94.172 kb on + strand, within Pf1N1B4_5357 at 94.172 kb on + strand, within Pf1N1B4_5357 at 94.173 kb on - strand, within Pf1N1B4_5357 at 94.333 kb on + strand at 94.334 kb on - strand at 94.344 kb on + strand at 94.345 kb on - strand at 94.361 kb on + strand at 94.386 kb on + strand at 94.387 kb on - strand at 94.528 kb on - strand at 94.530 kb on + strand at 94.530 kb on + strand at 94.530 kb on + strand at 94.530 kb on + strand at 94.530 kb on + strand at 94.530 kb on + strand at 94.531 kb on - strand at 94.531 kb on - strand at 94.532 kb on + strand at 94.532 kb on + strand at 94.532 kb on + strand at 94.532 kb on + strand at 94.532 kb on + strand at 94.532 kb on + strand at 94.532 kb on + strand at 94.532 kb on + strand at 94.533 kb on - strand at 94.600 kb on - strand at 94.662 kb on + strand at 94.662 kb on + strand at 94.662 kb on + strand at 94.662 kb on + strand at 94.662 kb on + strand at 94.662 kb on + strand at 94.662 kb on + strand at 94.662 kb on + strand at 94.663 kb on - strand at 94.663 kb on - strand at 94.707 kb on + strand at 94.752 kb on + strand at 94.752 kb on + strand at 94.752 kb on + strand at 94.752 kb on + strand at 94.752 kb on + strand at 94.752 kb on + strand at 94.752 kb on + strand at 94.752 kb on + strand at 94.753 kb on - strand at 94.753 kb on - strand at 94.825 kb on + strand at 94.825 kb on + strand at 94.825 kb on + strand at 94.825 kb on + strand at 94.825 kb on + strand at 94.862 kb on + strand at 94.863 kb on - strand, within Pf1N1B4_5358 at 94.939 kb on - strand, within Pf1N1B4_5358 at 94.996 kb on + strand at 95.073 kb on + strand at 95.073 kb on + strand at 95.079 kb on + strand at 95.079 kb on + strand at 95.080 kb on - strand at 95.080 kb on - strand at 95.145 kb on + strand, within Pf1N1B4_5359 at 95.145 kb on + strand, within Pf1N1B4_5359 at 95.160 kb on + strand, within Pf1N1B4_5359 at 95.213 kb on + strand, within Pf1N1B4_5359 at 95.213 kb on + strand, within Pf1N1B4_5359 at 95.213 kb on + strand, within Pf1N1B4_5359 at 95.213 kb on + strand, within Pf1N1B4_5359 at 95.214 kb on - strand, within Pf1N1B4_5359
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 93,147 + Pf1N1B4_5356 0.69 -0.1 93,147 + Pf1N1B4_5356 0.69 -0.7 93,185 + -0.3 93,186 - Pf1N1B4_5356 0.85 +0.9 93,220 + -0.5 93,283 - +0.7 93,315 + +0.4 93,315 + -0.3 93,315 + -0.3 93,316 - -0.5 93,466 - -0.2 93,529 + +0.7 93,529 + +0.7 93,529 + +1.2 93,529 + -0.4 93,529 + +0.7 93,529 + -0.1 93,529 + +0.5 93,529 + +0.6 93,529 + -0.4 93,529 + +1.7 93,529 + -1.6 93,529 + +0.7 93,529 + -1.3 93,529 + +0.1 93,529 + -0.3 93,529 + -0.1 93,529 + +0.4 93,530 - +1.0 93,530 - +0.9 93,530 - +1.2 93,530 - +1.1 93,640 + +1.3 93,680 + -0.2 93,760 - +1.7 93,769 + -1.6 93,941 + -0.3 93,941 + -0.7 93,984 + +0.9 93,985 - +0.1 93,985 - -0.1 94,078 - +1.7 94,086 + -0.7 94,086 + +0.3 94,096 + +0.1 94,172 + Pf1N1B4_5357 0.24 +0.7 94,172 + Pf1N1B4_5357 0.24 -1.1 94,173 - Pf1N1B4_5357 0.24 -0.6 94,333 + +0.4 94,334 - -1.6 94,344 + -0.3 94,345 - +0.2 94,361 + -0.2 94,386 + +0.5 94,387 - +0.7 94,528 - +0.4 94,530 + -2.6 94,530 + -0.5 94,530 + -0.5 94,530 + -0.3 94,530 + -0.1 94,530 + +0.1 94,531 - -0.9 94,531 - +0.4 94,532 + -0.1 94,532 + -0.6 94,532 + -0.9 94,532 + +0.3 94,532 + +2.9 94,532 + +0.6 94,532 + +0.7 94,532 + -0.8 94,533 - -0.3 94,600 - +0.5 94,662 + -0.7 94,662 + +0.7 94,662 + -0.0 94,662 + -0.3 94,662 + +0.5 94,662 + -1.6 94,662 + +0.1 94,662 + +0.9 94,663 - -0.1 94,663 - +0.0 94,707 + +0.1 94,752 + +0.4 94,752 + -1.3 94,752 + -1.6 94,752 + +0.3 94,752 + -0.9 94,752 + +0.4 94,752 + -0.6 94,752 + -0.6 94,753 - +1.0 94,753 - -1.1 94,825 + -0.6 94,825 + -1.6 94,825 + +1.0 94,825 + -0.9 94,825 + +1.1 94,862 + +0.2 94,863 - Pf1N1B4_5358 0.10 -0.9 94,939 - Pf1N1B4_5358 0.58 +1.5 94,996 + +0.2 95,073 + +0.2 95,073 + +0.3 95,079 + +0.3 95,079 + -0.3 95,080 - -0.6 95,080 - -0.1 95,145 + Pf1N1B4_5359 0.20 -0.3 95,145 + Pf1N1B4_5359 0.20 -0.1 95,160 + Pf1N1B4_5359 0.26 -0.2 95,213 + Pf1N1B4_5359 0.44 -0.1 95,213 + Pf1N1B4_5359 0.44 +0.4 95,213 + Pf1N1B4_5359 0.44 +0.4 95,213 + Pf1N1B4_5359 0.44 -0.1 95,214 - Pf1N1B4_5359 0.44 -0.1
Or see this region's nucleotide sequence