Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5293 and Pf1N1B4_5294 are separated by 58 nucleotides Pf1N1B4_5294 and Pf1N1B4_5295 are separated by 109 nucleotides Pf1N1B4_5295 and Pf1N1B4_5296 are separated by 195 nucleotides Pf1N1B4_5296 and Pf1N1B4_5297 are separated by 152 nucleotides
Pf1N1B4_5293 - hypothetical protein, at 49,892 to 50,107
_5293
Pf1N1B4_5294 - hypothetical protein, at 50,166 to 50,285
_5294
Pf1N1B4_5295 - hypothetical protein, at 50,395 to 50,679
_5295
Pf1N1B4_5296 - hypothetical protein, at 50,875 to 51,123
_5296
Pf1N1B4_5297 - hypothetical protein, at 51,276 to 51,413
_5297
Position (kb)
50
51 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 49.183 kb on - strand at 49.231 kb on + strand at 49.231 kb on + strand at 49.231 kb on + strand at 49.231 kb on + strand at 49.231 kb on + strand at 49.231 kb on + strand at 49.231 kb on + strand at 49.231 kb on + strand at 49.231 kb on + strand at 49.231 kb on + strand at 49.231 kb on + strand at 49.231 kb on + strand at 49.232 kb on - strand at 49.232 kb on - strand at 49.232 kb on - strand at 49.232 kb on - strand at 49.232 kb on - strand at 49.232 kb on - strand at 49.235 kb on + strand at 49.235 kb on + strand at 49.235 kb on + strand at 49.235 kb on + strand at 49.235 kb on + strand at 49.235 kb on + strand at 49.235 kb on + strand at 49.235 kb on + strand at 49.235 kb on + strand at 49.236 kb on - strand at 49.236 kb on - strand at 49.236 kb on - strand at 49.236 kb on - strand at 49.236 kb on - strand at 49.261 kb on + strand at 49.262 kb on - strand at 49.285 kb on + strand at 49.312 kb on + strand at 49.312 kb on + strand at 49.358 kb on + strand at 49.359 kb on - strand at 49.359 kb on - strand at 49.359 kb on - strand at 49.379 kb on + strand at 49.379 kb on + strand at 49.379 kb on + strand at 49.379 kb on + strand at 49.380 kb on - strand at 49.380 kb on - strand at 49.431 kb on + strand at 49.431 kb on + strand at 49.432 kb on - strand at 49.432 kb on - strand at 49.448 kb on + strand at 49.459 kb on + strand at 49.569 kb on + strand at 49.570 kb on - strand at 49.659 kb on + strand at 49.659 kb on + strand at 49.996 kb on + strand, within Pf1N1B4_5293 at 49.996 kb on + strand, within Pf1N1B4_5293 at 49.996 kb on + strand, within Pf1N1B4_5293 at 49.996 kb on + strand, within Pf1N1B4_5293 at 49.996 kb on + strand, within Pf1N1B4_5293 at 49.997 kb on - strand, within Pf1N1B4_5293 at 50.009 kb on - strand, within Pf1N1B4_5293 at 50.010 kb on + strand, within Pf1N1B4_5293 at 50.011 kb on - strand, within Pf1N1B4_5293 at 50.011 kb on - strand, within Pf1N1B4_5293 at 50.152 kb on + strand at 50.152 kb on + strand at 50.153 kb on - strand at 50.266 kb on - strand, within Pf1N1B4_5294 at 50.266 kb on - strand, within Pf1N1B4_5294 at 50.329 kb on + strand at 50.330 kb on - strand at 50.374 kb on + strand at 50.374 kb on + strand at 50.383 kb on + strand at 50.530 kb on + strand, within Pf1N1B4_5295 at 50.530 kb on + strand, within Pf1N1B4_5295 at 50.530 kb on + strand, within Pf1N1B4_5295 at 50.530 kb on + strand, within Pf1N1B4_5295 at 50.530 kb on + strand, within Pf1N1B4_5295 at 50.530 kb on + strand, within Pf1N1B4_5295 at 50.530 kb on + strand, within Pf1N1B4_5295 at 50.530 kb on + strand, within Pf1N1B4_5295 at 50.530 kb on + strand at 50.530 kb on + strand, within Pf1N1B4_5295 at 50.531 kb on - strand, within Pf1N1B4_5295 at 50.531 kb on - strand at 50.531 kb on - strand, within Pf1N1B4_5295 at 50.531 kb on - strand, within Pf1N1B4_5295 at 50.557 kb on - strand, within Pf1N1B4_5295 at 50.557 kb on - strand, within Pf1N1B4_5295 at 50.582 kb on + strand, within Pf1N1B4_5295 at 50.596 kb on - strand, within Pf1N1B4_5295 at 50.658 kb on + strand at 50.658 kb on + strand at 50.699 kb on - strand at 50.791 kb on + strand at 50.974 kb on + strand, within Pf1N1B4_5296 at 50.974 kb on + strand, within Pf1N1B4_5296 at 50.974 kb on + strand, within Pf1N1B4_5296 at 50.974 kb on + strand at 50.974 kb on + strand, within Pf1N1B4_5296 at 50.974 kb on + strand at 50.974 kb on + strand, within Pf1N1B4_5296 at 50.974 kb on + strand, within Pf1N1B4_5296 at 50.974 kb on + strand, within Pf1N1B4_5296 at 50.975 kb on - strand, within Pf1N1B4_5296 at 50.975 kb on - strand, within Pf1N1B4_5296 at 50.975 kb on - strand, within Pf1N1B4_5296 at 51.001 kb on + strand, within Pf1N1B4_5296 at 51.001 kb on + strand, within Pf1N1B4_5296 at 51.002 kb on - strand, within Pf1N1B4_5296 at 51.034 kb on - strand, within Pf1N1B4_5296 at 51.166 kb on - strand at 51.166 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 49,183 - -0.3 49,231 + +0.2 49,231 + +0.3 49,231 + +0.1 49,231 + -0.5 49,231 + -0.5 49,231 + -0.1 49,231 + +0.9 49,231 + +1.1 49,231 + +1.3 49,231 + +0.3 49,231 + +0.0 49,231 + +1.2 49,232 - +0.1 49,232 - +0.5 49,232 - -0.0 49,232 - -2.1 49,232 - +0.2 49,232 - -1.7 49,235 + +2.9 49,235 + +0.1 49,235 + -0.2 49,235 + +0.4 49,235 + -0.3 49,235 + +0.7 49,235 + +0.5 49,235 + +1.3 49,235 + +1.7 49,236 - -0.2 49,236 - +0.0 49,236 - -0.1 49,236 - -0.3 49,236 - +0.4 49,261 + -0.3 49,262 - +0.7 49,285 + +1.3 49,312 + -0.3 49,312 + -1.2 49,358 + +1.0 49,359 - -0.9 49,359 - +0.0 49,359 - -1.3 49,379 + +0.7 49,379 + +0.3 49,379 + -0.3 49,379 + -0.3 49,380 - +0.4 49,380 - +0.2 49,431 + -1.3 49,431 + -0.9 49,432 - -0.5 49,432 - -1.1 49,448 + +0.1 49,459 + -0.1 49,569 + -0.5 49,570 - -0.9 49,659 + +0.7 49,659 + -1.3 49,996 + Pf1N1B4_5293 0.48 +1.1 49,996 + Pf1N1B4_5293 0.48 -1.1 49,996 + Pf1N1B4_5293 0.48 +0.2 49,996 + Pf1N1B4_5293 0.48 +1.2 49,996 + Pf1N1B4_5293 0.48 -1.6 49,997 - Pf1N1B4_5293 0.49 -0.2 50,009 - Pf1N1B4_5293 0.54 -0.4 50,010 + Pf1N1B4_5293 0.55 +0.3 50,011 - Pf1N1B4_5293 0.55 -0.3 50,011 - Pf1N1B4_5293 0.55 -1.4 50,152 + +0.0 50,152 + +1.9 50,153 - +1.7 50,266 - Pf1N1B4_5294 0.83 -1.0 50,266 - Pf1N1B4_5294 0.83 -0.1 50,329 + +0.4 50,330 - -0.9 50,374 + +0.0 50,374 + +0.1 50,383 + +1.2 50,530 + Pf1N1B4_5295 0.47 +0.5 50,530 + Pf1N1B4_5295 0.47 -0.9 50,530 + Pf1N1B4_5295 0.47 +0.9 50,530 + Pf1N1B4_5295 0.47 +0.7 50,530 + Pf1N1B4_5295 0.47 +1.3 50,530 + Pf1N1B4_5295 0.47 +0.0 50,530 + Pf1N1B4_5295 0.47 -2.9 50,530 + Pf1N1B4_5295 0.47 -0.9 50,530 + -0.9 50,530 + Pf1N1B4_5295 0.47 -1.3 50,531 - Pf1N1B4_5295 0.48 +1.5 50,531 - -0.1 50,531 - Pf1N1B4_5295 0.48 +1.4 50,531 - Pf1N1B4_5295 0.48 -0.1 50,557 - Pf1N1B4_5295 0.57 -0.2 50,557 - Pf1N1B4_5295 0.57 +0.1 50,582 + Pf1N1B4_5295 0.66 +0.9 50,596 - Pf1N1B4_5295 0.71 -1.1 50,658 + -0.7 50,658 + +0.1 50,699 - +0.3 50,791 + -0.4 50,974 + Pf1N1B4_5296 0.40 +0.0 50,974 + Pf1N1B4_5296 0.40 +1.4 50,974 + Pf1N1B4_5296 0.40 -0.5 50,974 + +0.7 50,974 + Pf1N1B4_5296 0.40 +1.0 50,974 + -1.9 50,974 + Pf1N1B4_5296 0.40 +1.1 50,974 + Pf1N1B4_5296 0.40 +0.1 50,974 + Pf1N1B4_5296 0.40 +0.8 50,975 - Pf1N1B4_5296 0.40 -1.6 50,975 - Pf1N1B4_5296 0.40 -0.3 50,975 - Pf1N1B4_5296 0.40 -0.5 51,001 + Pf1N1B4_5296 0.51 +0.1 51,001 + Pf1N1B4_5296 0.51 +0.7 51,002 - Pf1N1B4_5296 0.51 -0.1 51,034 - Pf1N1B4_5296 0.64 -0.6 51,166 - -2.1 51,166 - +0.1
Or see this region's nucleotide sequence