Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5276 and Pf1N1B4_5277 are separated by 149 nucleotides Pf1N1B4_5277 and Pf1N1B4_5278 are separated by 155 nucleotides Pf1N1B4_5278 and Pf1N1B4_5279 are separated by 320 nucleotides Pf1N1B4_5279 and Pf1N1B4_5280 are separated by 160 nucleotides
Pf1N1B4_5276 - hypothetical protein, at 32,503 to 32,901
_5276
Pf1N1B4_5277 - Phage protein, at 33,051 to 33,326
_5277
Pf1N1B4_5278 - hypothetical protein, at 33,482 to 33,682
_5278
Pf1N1B4_5279 - hypothetical protein, at 34,003 to 34,209
_5279
Pf1N1B4_5280 - Nucleoid-associated protein, at 34,370 to 35,386
_5280
Position (kb) 33
34 Strain fitness (log2 ratio) -2
-1
0
1
2 at 32.521 kb on + strand at 32.521 kb on + strand at 32.521 kb on + strand at 32.860 kb on + strand, within Pf1N1B4_5276 at 32.860 kb on + strand, within Pf1N1B4_5276 at 32.860 kb on + strand, within Pf1N1B4_5276 at 32.943 kb on + strand at 32.944 kb on - strand at 33.084 kb on + strand at 33.084 kb on + strand, within Pf1N1B4_5277 at 33.085 kb on - strand, within Pf1N1B4_5277 at 33.085 kb on - strand, within Pf1N1B4_5277 at 33.188 kb on + strand, within Pf1N1B4_5277 at 33.235 kb on + strand, within Pf1N1B4_5277 at 33.236 kb on - strand, within Pf1N1B4_5277 at 33.236 kb on - strand, within Pf1N1B4_5277 at 33.263 kb on + strand, within Pf1N1B4_5277 at 33.263 kb on + strand, within Pf1N1B4_5277 at 33.264 kb on - strand, within Pf1N1B4_5277 at 33.266 kb on + strand, within Pf1N1B4_5277 at 33.266 kb on + strand, within Pf1N1B4_5277 at 33.267 kb on - strand, within Pf1N1B4_5277 at 33.361 kb on - strand at 33.361 kb on - strand at 33.484 kb on - strand at 33.484 kb on - strand at 33.627 kb on - strand, within Pf1N1B4_5278 at 33.650 kb on + strand at 33.650 kb on + strand at 33.650 kb on + strand, within Pf1N1B4_5278 at 33.651 kb on - strand, within Pf1N1B4_5278 at 33.651 kb on - strand at 33.651 kb on - strand, within Pf1N1B4_5278 at 33.765 kb on + strand at 33.838 kb on + strand at 33.953 kb on + strand at 33.969 kb on - strand at 33.993 kb on + strand at 33.994 kb on - strand at 34.022 kb on + strand at 34.085 kb on + strand, within Pf1N1B4_5279 at 34.102 kb on + strand, within Pf1N1B4_5279 at 34.102 kb on + strand, within Pf1N1B4_5279 at 34.102 kb on + strand, within Pf1N1B4_5279 at 34.102 kb on + strand, within Pf1N1B4_5279 at 34.103 kb on - strand, within Pf1N1B4_5279 at 34.103 kb on - strand at 34.213 kb on + strand at 34.213 kb on + strand at 34.214 kb on - strand at 34.214 kb on - strand at 34.214 kb on - strand at 34.267 kb on + strand at 34.267 kb on + strand at 34.268 kb on - strand at 34.268 kb on - strand at 34.268 kb on - strand at 34.605 kb on + strand, within Pf1N1B4_5280 at 34.606 kb on - strand, within Pf1N1B4_5280
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 32,521 + +0.7 32,521 + -0.8 32,521 + +0.7 32,860 + Pf1N1B4_5276 0.89 -0.6 32,860 + Pf1N1B4_5276 0.89 +0.4 32,860 + Pf1N1B4_5276 0.89 -0.9 32,943 + -0.1 32,944 - -1.1 33,084 + -0.3 33,084 + Pf1N1B4_5277 0.12 +1.0 33,085 - Pf1N1B4_5277 0.12 +0.5 33,085 - Pf1N1B4_5277 0.12 +0.3 33,188 + Pf1N1B4_5277 0.50 -0.4 33,235 + Pf1N1B4_5277 0.67 +0.5 33,236 - Pf1N1B4_5277 0.67 +0.4 33,236 - Pf1N1B4_5277 0.67 +1.0 33,263 + Pf1N1B4_5277 0.77 -0.4 33,263 + Pf1N1B4_5277 0.77 +0.3 33,264 - Pf1N1B4_5277 0.77 +0.8 33,266 + Pf1N1B4_5277 0.78 +0.7 33,266 + Pf1N1B4_5277 0.78 +0.9 33,267 - Pf1N1B4_5277 0.78 -0.3 33,361 - +1.3 33,361 - +0.6 33,484 - +0.7 33,484 - -0.1 33,627 - Pf1N1B4_5278 0.72 -1.3 33,650 + +2.7 33,650 + -0.3 33,650 + Pf1N1B4_5278 0.84 +0.1 33,651 - Pf1N1B4_5278 0.84 -1.6 33,651 - -0.9 33,651 - Pf1N1B4_5278 0.84 -0.2 33,765 + +1.9 33,838 + -0.9 33,953 + -1.1 33,969 - -0.9 33,993 + +0.0 33,994 - +0.6 34,022 + -0.1 34,085 + Pf1N1B4_5279 0.40 -2.3 34,102 + Pf1N1B4_5279 0.48 +0.4 34,102 + Pf1N1B4_5279 0.48 -1.0 34,102 + Pf1N1B4_5279 0.48 -0.3 34,102 + Pf1N1B4_5279 0.48 +0.7 34,103 - Pf1N1B4_5279 0.48 -1.2 34,103 - +1.4 34,213 + -2.5 34,213 + -0.6 34,214 - +0.9 34,214 - +1.2 34,214 - +0.1 34,267 + +0.2 34,267 + -0.1 34,268 - +1.9 34,268 - +0.4 34,268 - -0.9 34,605 + Pf1N1B4_5280 0.23 +0.3 34,606 - Pf1N1B4_5280 0.23 +0.5
Or see this region's nucleotide sequence