Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5255 and Pf1N1B4_5256 are separated by 149 nucleotides Pf1N1B4_5256 and Pf1N1B4_5257 are separated by 546 nucleotides
Pf1N1B4_5255 - hypothetical protein, at 14,928 to 15,152
_5255
Pf1N1B4_5256 - hypothetical protein, at 15,302 to 15,460
_5256
Pf1N1B4_5257 - hypothetical protein, at 16,007 to 16,306
_5257
Position (kb) 14
15
16 Strain fitness (log2 ratio) -2
-1
0
1
2 at 13.964 kb on - strand at 14.101 kb on + strand at 14.148 kb on + strand at 14.148 kb on + strand at 14.149 kb on - strand at 14.149 kb on - strand at 14.149 kb on - strand at 14.149 kb on - strand at 14.149 kb on - strand at 14.162 kb on + strand at 14.162 kb on + strand at 14.163 kb on - strand at 14.163 kb on - strand at 14.246 kb on + strand at 14.364 kb on - strand at 14.364 kb on - strand at 14.364 kb on - strand at 14.364 kb on - strand at 14.401 kb on + strand at 14.401 kb on + strand at 14.401 kb on + strand at 14.402 kb on - strand at 14.539 kb on + strand at 14.539 kb on + strand at 14.604 kb on + strand at 14.605 kb on - strand at 14.612 kb on - strand at 14.668 kb on + strand at 14.669 kb on - strand at 14.669 kb on - strand at 14.669 kb on - strand at 14.669 kb on - strand at 14.669 kb on - strand at 14.670 kb on + strand at 14.670 kb on + strand at 14.670 kb on + strand at 14.670 kb on + strand at 14.670 kb on + strand at 14.670 kb on + strand at 14.670 kb on + strand at 14.670 kb on + strand at 14.670 kb on + strand at 14.670 kb on + strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.671 kb on - strand at 14.675 kb on - strand at 14.692 kb on + strand at 14.692 kb on + strand at 14.693 kb on - strand at 14.693 kb on - strand at 14.701 kb on - strand at 14.701 kb on - strand at 14.701 kb on - strand at 14.701 kb on - strand at 14.706 kb on + strand at 14.706 kb on + strand at 14.707 kb on - strand at 14.707 kb on - strand at 14.895 kb on - strand at 14.917 kb on - strand at 14.985 kb on - strand, within Pf1N1B4_5255 at 15.028 kb on + strand, within Pf1N1B4_5255 at 15.073 kb on + strand, within Pf1N1B4_5255 at 15.074 kb on - strand, within Pf1N1B4_5255 at 15.125 kb on + strand, within Pf1N1B4_5255 at 15.126 kb on - strand, within Pf1N1B4_5255 at 15.126 kb on - strand, within Pf1N1B4_5255 at 15.126 kb on - strand, within Pf1N1B4_5255 at 15.249 kb on - strand at 15.298 kb on - strand at 15.303 kb on + strand at 15.332 kb on + strand, within Pf1N1B4_5256 at 15.333 kb on - strand, within Pf1N1B4_5256 at 15.498 kb on + strand at 15.498 kb on + strand at 15.498 kb on + strand at 15.498 kb on + strand at 15.498 kb on + strand at 15.498 kb on + strand at 15.499 kb on - strand at 15.499 kb on - strand at 15.581 kb on - strand at 15.604 kb on + strand at 15.757 kb on + strand at 15.758 kb on - strand at 15.758 kb on - strand at 15.794 kb on + strand at 15.794 kb on + strand at 15.889 kb on + strand at 15.889 kb on + strand at 15.889 kb on + strand at 15.889 kb on + strand at 15.889 kb on + strand at 15.889 kb on + strand at 15.889 kb on + strand at 15.889 kb on + strand at 15.889 kb on + strand at 15.889 kb on + strand at 15.890 kb on - strand at 15.890 kb on - strand at 15.890 kb on - strand at 15.890 kb on - strand at 15.890 kb on - strand at 15.890 kb on - strand at 15.890 kb on - strand at 15.890 kb on - strand at 15.890 kb on - strand at 16.037 kb on + strand, within Pf1N1B4_5257 at 16.037 kb on + strand, within Pf1N1B4_5257 at 16.037 kb on + strand at 16.037 kb on + strand, within Pf1N1B4_5257 at 16.038 kb on - strand, within Pf1N1B4_5257 at 16.038 kb on - strand, within Pf1N1B4_5257 at 16.038 kb on - strand, within Pf1N1B4_5257 at 16.038 kb on - strand, within Pf1N1B4_5257
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 13,964 - -0.9 14,101 + +0.7 14,148 + +1.3 14,148 + -2.0 14,149 - +2.1 14,149 - +0.2 14,149 - +0.3 14,149 - +0.5 14,149 - +0.2 14,162 + +1.1 14,162 + -0.3 14,163 - +1.1 14,163 - -1.1 14,246 + -0.9 14,364 - +0.3 14,364 - -1.1 14,364 - +0.7 14,364 - -1.8 14,401 + +0.2 14,401 + +0.1 14,401 + -0.9 14,402 - -0.3 14,539 + -0.3 14,539 + -1.6 14,604 + +0.1 14,605 - +1.1 14,612 - +0.6 14,668 + -0.7 14,669 - +0.9 14,669 - -1.9 14,669 - +0.3 14,669 - -0.4 14,669 - -0.7 14,670 + -0.3 14,670 + -0.5 14,670 + +0.7 14,670 + +0.3 14,670 + +1.3 14,670 + -0.4 14,670 + -1.9 14,670 + +0.3 14,670 + +0.1 14,670 + -1.0 14,671 - +0.7 14,671 - +0.3 14,671 - -0.9 14,671 - +0.2 14,671 - +0.9 14,671 - -0.7 14,671 - +0.6 14,671 - -0.2 14,671 - +0.1 14,671 - -0.3 14,671 - +0.9 14,671 - +0.9 14,671 - +0.0 14,671 - -0.1 14,671 - +0.9 14,671 - +0.1 14,671 - -0.1 14,671 - -0.1 14,671 - +0.9 14,671 - +0.7 14,671 - +0.7 14,671 - -2.6 14,675 - +0.5 14,692 + +2.3 14,692 + +0.3 14,693 - +0.5 14,693 - +1.4 14,701 - -0.3 14,701 - +0.7 14,701 - -1.3 14,701 - +2.3 14,706 + -0.3 14,706 + -1.9 14,707 - +0.7 14,707 - -1.1 14,895 - -0.3 14,917 - +1.1 14,985 - Pf1N1B4_5255 0.25 -1.9 15,028 + Pf1N1B4_5255 0.44 -2.1 15,073 + Pf1N1B4_5255 0.64 -0.8 15,074 - Pf1N1B4_5255 0.65 -0.5 15,125 + Pf1N1B4_5255 0.88 +1.4 15,126 - Pf1N1B4_5255 0.88 -0.1 15,126 - Pf1N1B4_5255 0.88 -0.9 15,126 - Pf1N1B4_5255 0.88 -0.3 15,249 - +2.7 15,298 - -0.9 15,303 + +0.5 15,332 + Pf1N1B4_5256 0.19 +1.7 15,333 - Pf1N1B4_5256 0.19 +0.7 15,498 + +0.7 15,498 + +0.5 15,498 + -2.1 15,498 + +0.0 15,498 + +1.5 15,498 + -0.1 15,499 - +0.2 15,499 - +0.2 15,581 - -1.3 15,604 + -0.2 15,757 + -0.6 15,758 - -0.4 15,758 - +0.1 15,794 + +0.2 15,794 + +0.4 15,889 + +0.8 15,889 + -0.6 15,889 + -0.0 15,889 + -0.3 15,889 + -0.5 15,889 + +0.7 15,889 + +0.6 15,889 + -0.6 15,889 + +0.3 15,889 + -0.7 15,890 - +0.1 15,890 - +1.3 15,890 - +0.3 15,890 - -1.6 15,890 - +1.7 15,890 - -0.7 15,890 - +1.3 15,890 - -0.3 15,890 - +0.4 16,037 + Pf1N1B4_5257 0.10 +0.1 16,037 + Pf1N1B4_5257 0.10 -0.9 16,037 + +1.3 16,037 + Pf1N1B4_5257 0.10 +0.0 16,038 - Pf1N1B4_5257 0.10 +0.7 16,038 - Pf1N1B4_5257 0.10 -0.1 16,038 - Pf1N1B4_5257 0.10 +0.2 16,038 - Pf1N1B4_5257 0.10 +0.2
Or see this region's nucleotide sequence