Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1814 and Pf1N1B4_5187 are separated by 63 nucleotides Pf1N1B4_5187 and Pf1N1B4_5188 are separated by 156 nucleotides
Pf1N1B4_1814 - Sulfite reductase [NADPH] flavoprotein alpha-component (EC 1.8.1.2), at 2,085,058 to 2,086,254
_1814
Pf1N1B4_5187 - 5S RNA, at 2,086,318 to 2,086,437
_5187
Pf1N1B4_5188 - Large Subunit Ribosomal RNA; lsuRNA; LSU rRNA, at 2,086,594 to 2,089,486
_5188
Position (kb)
2086
2087 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2085.382 kb on + strand, within Pf1N1B4_1814 at 2085.383 kb on - strand, within Pf1N1B4_1814 at 2085.383 kb on - strand, within Pf1N1B4_1814 at 2085.383 kb on - strand, within Pf1N1B4_1814 at 2085.383 kb on - strand, within Pf1N1B4_1814 at 2085.383 kb on - strand, within Pf1N1B4_1814 at 2085.481 kb on + strand, within Pf1N1B4_1814 at 2085.482 kb on - strand, within Pf1N1B4_1814 at 2085.482 kb on - strand, within Pf1N1B4_1814 at 2085.482 kb on - strand, within Pf1N1B4_1814 at 2085.501 kb on + strand, within Pf1N1B4_1814 at 2085.501 kb on + strand, within Pf1N1B4_1814 at 2085.502 kb on - strand, within Pf1N1B4_1814 at 2085.834 kb on + strand, within Pf1N1B4_1814 at 2085.859 kb on + strand, within Pf1N1B4_1814 at 2085.859 kb on + strand, within Pf1N1B4_1814 at 2085.860 kb on - strand, within Pf1N1B4_1814 at 2085.919 kb on + strand, within Pf1N1B4_1814 at 2085.919 kb on + strand, within Pf1N1B4_1814 at 2085.919 kb on + strand, within Pf1N1B4_1814 at 2085.919 kb on + strand, within Pf1N1B4_1814 at 2085.919 kb on + strand, within Pf1N1B4_1814 at 2085.919 kb on + strand, within Pf1N1B4_1814 at 2085.919 kb on + strand, within Pf1N1B4_1814 at 2085.920 kb on - strand, within Pf1N1B4_1814 at 2085.920 kb on - strand, within Pf1N1B4_1814 at 2085.920 kb on - strand, within Pf1N1B4_1814 at 2085.920 kb on - strand, within Pf1N1B4_1814 at 2085.920 kb on - strand, within Pf1N1B4_1814 at 2085.920 kb on - strand, within Pf1N1B4_1814 at 2085.920 kb on - strand, within Pf1N1B4_1814 at 2085.920 kb on - strand, within Pf1N1B4_1814 at 2085.922 kb on + strand, within Pf1N1B4_1814 at 2085.923 kb on - strand, within Pf1N1B4_1814 at 2085.923 kb on - strand, within Pf1N1B4_1814 at 2085.923 kb on - strand, within Pf1N1B4_1814 at 2085.943 kb on + strand, within Pf1N1B4_1814 at 2086.139 kb on + strand at 2086.139 kb on + strand at 2086.139 kb on + strand at 2086.139 kb on + strand at 2086.157 kb on + strand at 2086.157 kb on + strand at 2086.158 kb on - strand at 2086.158 kb on - strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.177 kb on + strand at 2086.178 kb on - strand at 2086.178 kb on - strand at 2086.178 kb on - strand at 2086.178 kb on - strand at 2086.179 kb on + strand at 2086.212 kb on + strand at 2086.213 kb on - strand at 2086.224 kb on + strand at 2086.224 kb on + strand at 2086.224 kb on + strand at 2086.224 kb on + strand at 2086.224 kb on + strand at 2086.224 kb on + strand at 2086.224 kb on + strand at 2086.224 kb on + strand at 2086.225 kb on - strand at 2086.261 kb on + strand at 2086.261 kb on + strand at 2086.261 kb on + strand at 2086.261 kb on + strand at 2086.293 kb on + strand at 2086.294 kb on - strand at 2086.335 kb on - strand, within Pf1N1B4_5187 at 2086.335 kb on - strand, within Pf1N1B4_5187 at 2086.593 kb on - strand at 2086.595 kb on - strand at 2086.595 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 2,085,382 + Pf1N1B4_1814 0.27 +0.2 2,085,383 - Pf1N1B4_1814 0.27 -0.3 2,085,383 - Pf1N1B4_1814 0.27 -0.9 2,085,383 - Pf1N1B4_1814 0.27 +0.1 2,085,383 - Pf1N1B4_1814 0.27 +2.2 2,085,383 - Pf1N1B4_1814 0.27 -0.1 2,085,481 + Pf1N1B4_1814 0.35 +2.2 2,085,482 - Pf1N1B4_1814 0.35 +1.7 2,085,482 - Pf1N1B4_1814 0.35 +1.6 2,085,482 - Pf1N1B4_1814 0.35 +1.4 2,085,501 + Pf1N1B4_1814 0.37 -0.0 2,085,501 + Pf1N1B4_1814 0.37 -0.1 2,085,502 - Pf1N1B4_1814 0.37 -0.3 2,085,834 + Pf1N1B4_1814 0.65 -1.1 2,085,859 + Pf1N1B4_1814 0.67 +0.2 2,085,859 + Pf1N1B4_1814 0.67 -1.2 2,085,860 - Pf1N1B4_1814 0.67 +0.2 2,085,919 + Pf1N1B4_1814 0.72 +0.2 2,085,919 + Pf1N1B4_1814 0.72 -0.3 2,085,919 + Pf1N1B4_1814 0.72 -0.1 2,085,919 + Pf1N1B4_1814 0.72 -1.9 2,085,919 + Pf1N1B4_1814 0.72 -0.6 2,085,919 + Pf1N1B4_1814 0.72 -0.5 2,085,919 + Pf1N1B4_1814 0.72 -0.7 2,085,920 - Pf1N1B4_1814 0.72 +1.2 2,085,920 - Pf1N1B4_1814 0.72 -1.1 2,085,920 - Pf1N1B4_1814 0.72 -1.3 2,085,920 - Pf1N1B4_1814 0.72 -0.9 2,085,920 - Pf1N1B4_1814 0.72 +0.7 2,085,920 - Pf1N1B4_1814 0.72 -1.8 2,085,920 - Pf1N1B4_1814 0.72 +0.9 2,085,920 - Pf1N1B4_1814 0.72 +1.4 2,085,922 + Pf1N1B4_1814 0.72 +1.6 2,085,923 - Pf1N1B4_1814 0.72 +1.2 2,085,923 - Pf1N1B4_1814 0.72 -1.5 2,085,923 - Pf1N1B4_1814 0.72 +0.2 2,085,943 + Pf1N1B4_1814 0.74 -0.1 2,086,139 + +0.2 2,086,139 + +0.8 2,086,139 + -0.0 2,086,139 + +1.1 2,086,157 + +0.2 2,086,157 + +0.2 2,086,158 - +0.2 2,086,158 - -0.9 2,086,177 + +1.5 2,086,177 + +0.1 2,086,177 + -0.5 2,086,177 + +0.1 2,086,177 + -0.3 2,086,177 + +0.8 2,086,177 + +1.2 2,086,177 + -0.8 2,086,177 + +2.0 2,086,177 + +0.0 2,086,177 + -0.8 2,086,177 + +1.0 2,086,177 + +0.7 2,086,177 + +0.1 2,086,177 + -0.3 2,086,177 + -1.6 2,086,177 + +0.7 2,086,177 + +0.2 2,086,177 + +0.9 2,086,177 + -1.7 2,086,177 + +1.2 2,086,177 + +0.9 2,086,178 - +0.2 2,086,178 - +0.8 2,086,178 - -0.3 2,086,178 - -1.1 2,086,179 + +2.2 2,086,212 + +0.6 2,086,213 - -1.9 2,086,224 + -0.5 2,086,224 + -0.8 2,086,224 + -0.5 2,086,224 + +0.8 2,086,224 + +0.9 2,086,224 + +0.9 2,086,224 + -1.1 2,086,224 + +0.9 2,086,225 - -2.6 2,086,261 + -1.8 2,086,261 + -0.2 2,086,261 + +0.9 2,086,261 + -0.8 2,086,293 + +0.6 2,086,294 - -0.1 2,086,335 - Pf1N1B4_5187 0.14 +0.2 2,086,335 - Pf1N1B4_5187 0.14 -2.1 2,086,593 - -0.4 2,086,595 - +0.7 2,086,595 - +2.2
Or see this region's nucleotide sequence