Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5144 and Pf1N1B4_5145 are separated by 191 nucleotides Pf1N1B4_5145 and Pf1N1B4_5146 are separated by 191 nucleotides
Pf1N1B4_5144 - hypothetical protein, at 5,726,455 to 5,726,580
_5144
Pf1N1B4_5145 - Permeases of the major facilitator superfamily, at 5,726,772 to 5,728,298
_5145
Pf1N1B4_5146 - Chromosome initiation inhibitor, at 5,728,490 to 5,729,350
_5146
Position (kb)
5726
5727
5728
5729 Strain fitness (log2 ratio)
-2
-1
0
1 at 5725.803 kb on + strand at 5725.804 kb on - strand at 5725.811 kb on + strand at 5725.820 kb on - strand at 5725.823 kb on + strand at 5725.824 kb on - strand at 5725.824 kb on - strand at 5725.824 kb on - strand at 5725.824 kb on - strand at 5725.824 kb on - strand at 5725.883 kb on + strand at 5726.202 kb on - strand at 5726.768 kb on - strand at 5726.854 kb on + strand at 5726.982 kb on + strand, within Pf1N1B4_5145 at 5726.982 kb on + strand at 5726.982 kb on + strand, within Pf1N1B4_5145 at 5726.983 kb on - strand at 5727.010 kb on - strand, within Pf1N1B4_5145 at 5727.059 kb on + strand, within Pf1N1B4_5145 at 5727.089 kb on + strand, within Pf1N1B4_5145 at 5727.090 kb on - strand, within Pf1N1B4_5145 at 5727.101 kb on + strand, within Pf1N1B4_5145 at 5727.279 kb on + strand, within Pf1N1B4_5145 at 5727.280 kb on - strand, within Pf1N1B4_5145 at 5727.280 kb on - strand, within Pf1N1B4_5145 at 5727.420 kb on - strand, within Pf1N1B4_5145 at 5727.431 kb on - strand, within Pf1N1B4_5145 at 5727.442 kb on + strand, within Pf1N1B4_5145 at 5727.487 kb on - strand at 5727.547 kb on + strand, within Pf1N1B4_5145 at 5727.548 kb on - strand, within Pf1N1B4_5145 at 5727.548 kb on - strand, within Pf1N1B4_5145 at 5727.666 kb on + strand, within Pf1N1B4_5145 at 5727.667 kb on - strand, within Pf1N1B4_5145 at 5727.667 kb on - strand at 5727.667 kb on - strand, within Pf1N1B4_5145 at 5727.969 kb on - strand, within Pf1N1B4_5145 at 5727.969 kb on - strand, within Pf1N1B4_5145 at 5727.969 kb on - strand, within Pf1N1B4_5145 at 5727.969 kb on - strand, within Pf1N1B4_5145 at 5728.157 kb on + strand at 5728.157 kb on + strand at 5728.158 kb on - strand at 5728.393 kb on - strand at 5728.423 kb on + strand at 5728.489 kb on - strand at 5728.510 kb on - strand at 5728.860 kb on + strand, within Pf1N1B4_5146 at 5729.000 kb on - strand, within Pf1N1B4_5146 at 5729.000 kb on - strand, within Pf1N1B4_5146 at 5729.000 kb on - strand at 5729.119 kb on + strand, within Pf1N1B4_5146 at 5729.267 kb on - strand at 5729.268 kb on + strand at 5729.269 kb on - strand at 5729.269 kb on - strand at 5729.269 kb on - strand at 5729.269 kb on - strand at 5729.269 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 5,725,803 + -0.1 5,725,804 - -0.7 5,725,811 + +1.0 5,725,820 - -0.0 5,725,823 + -0.2 5,725,824 - -0.3 5,725,824 - -1.2 5,725,824 - +0.7 5,725,824 - -2.6 5,725,824 - -2.1 5,725,883 + +0.7 5,726,202 - +0.3 5,726,768 - -0.3 5,726,854 + -0.8 5,726,982 + Pf1N1B4_5145 0.14 +0.3 5,726,982 + -2.6 5,726,982 + Pf1N1B4_5145 0.14 +0.3 5,726,983 - +0.3 5,727,010 - Pf1N1B4_5145 0.16 -0.0 5,727,059 + Pf1N1B4_5145 0.19 +0.3 5,727,089 + Pf1N1B4_5145 0.21 -1.1 5,727,090 - Pf1N1B4_5145 0.21 -0.8 5,727,101 + Pf1N1B4_5145 0.22 -2.2 5,727,279 + Pf1N1B4_5145 0.33 +0.6 5,727,280 - Pf1N1B4_5145 0.33 -2.7 5,727,280 - Pf1N1B4_5145 0.33 -2.3 5,727,420 - Pf1N1B4_5145 0.42 -0.2 5,727,431 - Pf1N1B4_5145 0.43 +0.3 5,727,442 + Pf1N1B4_5145 0.44 -1.2 5,727,487 - -1.7 5,727,547 + Pf1N1B4_5145 0.51 -2.0 5,727,548 - Pf1N1B4_5145 0.51 -0.9 5,727,548 - Pf1N1B4_5145 0.51 -1.2 5,727,666 + Pf1N1B4_5145 0.59 -0.7 5,727,667 - Pf1N1B4_5145 0.59 -2.1 5,727,667 - +0.3 5,727,667 - Pf1N1B4_5145 0.59 +0.3 5,727,969 - Pf1N1B4_5145 0.78 -1.7 5,727,969 - Pf1N1B4_5145 0.78 -1.0 5,727,969 - Pf1N1B4_5145 0.78 +0.7 5,727,969 - Pf1N1B4_5145 0.78 -0.3 5,728,157 + +0.1 5,728,157 + +0.6 5,728,158 - -2.7 5,728,393 - +0.2 5,728,423 + +0.6 5,728,489 - +0.3 5,728,510 - +1.3 5,728,860 + Pf1N1B4_5146 0.43 +0.3 5,729,000 - Pf1N1B4_5146 0.59 +0.5 5,729,000 - Pf1N1B4_5146 0.59 +0.5 5,729,000 - +1.3 5,729,119 + Pf1N1B4_5146 0.73 -0.6 5,729,267 - +0.8 5,729,268 + +0.1 5,729,269 - -1.4 5,729,269 - -0.6 5,729,269 - -0.2 5,729,269 - +0.1 5,729,269 - -0.5
Or see this region's nucleotide sequence