Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5124 and Pf1N1B4_5125 are separated by 639 nucleotides Pf1N1B4_5125 and Pf1N1B4_5126 are separated by 2 nucleotides
Pf1N1B4_5124 - two component transcriptional regulator, LuxR family, at 5,700,301 to 5,700,918
_5124
Pf1N1B4_5125 - Type I secretion outer membrane protein, TolC precursor, at 5,701,558 to 5,702,937
_5125
Pf1N1B4_5126 - HlyD family secretion protein, at 5,702,940 to 5,704,253
_5126
Position (kb) 5701
5702
5703 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5700.574 kb on + strand, within Pf1N1B4_5124 at 5700.575 kb on - strand at 5700.576 kb on + strand, within Pf1N1B4_5124 at 5700.577 kb on - strand at 5700.890 kb on + strand at 5700.891 kb on - strand at 5700.994 kb on + strand at 5700.995 kb on - strand at 5701.045 kb on + strand at 5701.046 kb on - strand at 5701.048 kb on - strand at 5702.009 kb on + strand at 5702.042 kb on + strand, within Pf1N1B4_5125 at 5702.396 kb on + strand, within Pf1N1B4_5125 at 5702.576 kb on + strand, within Pf1N1B4_5125 at 5702.600 kb on + strand, within Pf1N1B4_5125 at 5702.600 kb on + strand, within Pf1N1B4_5125 at 5702.600 kb on + strand, within Pf1N1B4_5125 at 5702.600 kb on + strand, within Pf1N1B4_5125 at 5702.601 kb on - strand, within Pf1N1B4_5125 at 5702.606 kb on + strand, within Pf1N1B4_5125 at 5702.606 kb on + strand at 5702.617 kb on + strand, within Pf1N1B4_5125 at 5702.643 kb on - strand at 5702.765 kb on + strand, within Pf1N1B4_5125 at 5702.765 kb on + strand, within Pf1N1B4_5125 at 5702.765 kb on + strand, within Pf1N1B4_5125 at 5702.766 kb on - strand, within Pf1N1B4_5125 at 5702.807 kb on + strand at 5702.808 kb on - strand at 5702.808 kb on - strand at 5702.808 kb on - strand at 5703.027 kb on + strand at 5703.028 kb on - strand at 5703.110 kb on - strand, within Pf1N1B4_5126 at 5703.110 kb on - strand, within Pf1N1B4_5126 at 5703.112 kb on + strand, within Pf1N1B4_5126 at 5703.113 kb on - strand at 5703.395 kb on + strand, within Pf1N1B4_5126 at 5703.442 kb on + strand, within Pf1N1B4_5126 at 5703.442 kb on + strand, within Pf1N1B4_5126 at 5703.443 kb on - strand, within Pf1N1B4_5126 at 5703.510 kb on + strand, within Pf1N1B4_5126 at 5703.510 kb on + strand, within Pf1N1B4_5126 at 5703.511 kb on - strand, within Pf1N1B4_5126 at 5703.554 kb on + strand, within Pf1N1B4_5126 at 5703.807 kb on + strand, within Pf1N1B4_5126 at 5703.807 kb on + strand, within Pf1N1B4_5126 at 5703.861 kb on + strand, within Pf1N1B4_5126
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 5,700,574 + Pf1N1B4_5124 0.44 +0.3 5,700,575 - +0.3 5,700,576 + Pf1N1B4_5124 0.44 -0.6 5,700,577 - -0.7 5,700,890 + +0.7 5,700,891 - -0.2 5,700,994 + +1.1 5,700,995 - +1.7 5,701,045 + +0.3 5,701,046 - -0.7 5,701,048 - -0.2 5,702,009 + +0.8 5,702,042 + Pf1N1B4_5125 0.35 +0.3 5,702,396 + Pf1N1B4_5125 0.61 -0.7 5,702,576 + Pf1N1B4_5125 0.74 -0.4 5,702,600 + Pf1N1B4_5125 0.76 -1.7 5,702,600 + Pf1N1B4_5125 0.76 +0.3 5,702,600 + Pf1N1B4_5125 0.76 +0.5 5,702,600 + Pf1N1B4_5125 0.76 +0.1 5,702,601 - Pf1N1B4_5125 0.76 -1.3 5,702,606 + Pf1N1B4_5125 0.76 +0.4 5,702,606 + +0.3 5,702,617 + Pf1N1B4_5125 0.77 +0.3 5,702,643 - +0.3 5,702,765 + Pf1N1B4_5125 0.87 +0.7 5,702,765 + Pf1N1B4_5125 0.87 -0.6 5,702,765 + Pf1N1B4_5125 0.87 +1.1 5,702,766 - Pf1N1B4_5125 0.88 +0.4 5,702,807 + +3.3 5,702,808 - -0.6 5,702,808 - +0.6 5,702,808 - -0.7 5,703,027 + -2.1 5,703,028 - +0.3 5,703,110 - Pf1N1B4_5126 0.13 +0.3 5,703,110 - Pf1N1B4_5126 0.13 +2.8 5,703,112 + Pf1N1B4_5126 0.13 -0.3 5,703,113 - +0.7 5,703,395 + Pf1N1B4_5126 0.35 -0.4 5,703,442 + Pf1N1B4_5126 0.38 -1.0 5,703,442 + Pf1N1B4_5126 0.38 -1.3 5,703,443 - Pf1N1B4_5126 0.38 -0.3 5,703,510 + Pf1N1B4_5126 0.43 -0.1 5,703,510 + Pf1N1B4_5126 0.43 +0.4 5,703,511 - Pf1N1B4_5126 0.43 -0.3 5,703,554 + Pf1N1B4_5126 0.47 -2.1 5,703,807 + Pf1N1B4_5126 0.66 +0.1 5,703,807 + Pf1N1B4_5126 0.66 +0.6 5,703,861 + Pf1N1B4_5126 0.70 +1.0
Or see this region's nucleotide sequence