Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5117 and Pf1N1B4_5118 are separated by 52 nucleotides Pf1N1B4_5118 and Pf1N1B4_5119 are separated by 542 nucleotides Pf1N1B4_5119 and Pf1N1B4_5120 are separated by 12 nucleotides Pf1N1B4_5120 and Pf1N1B4_5121 are separated by 147 nucleotides
Pf1N1B4_5117 - Sucrose specific transcriptional regulator CscR, LacI family, at 5,677,470 to 5,678,453
_5117
Pf1N1B4_5118 - hypothetical protein, at 5,678,506 to 5,678,868
_5118
Pf1N1B4_5119 - Putative hemagglutinin/hemolysin-related protein, at 5,679,411 to 5,682,128
_5119
Pf1N1B4_5120 - hypothetical protein, at 5,682,141 to 5,682,260
_5120
Pf1N1B4_5121 - hypothetical protein, at 5,682,408 to 5,687,864
_5121
Position (kb)
5679
5680
5681
5682
5683 Strain fitness (log2 ratio)
-1
0
1
2 at 5678.709 kb on - strand at 5678.709 kb on - strand, within Pf1N1B4_5118 at 5678.868 kb on + strand at 5678.869 kb on - strand at 5678.892 kb on + strand at 5678.895 kb on + strand at 5679.007 kb on + strand at 5679.008 kb on - strand at 5679.008 kb on - strand at 5679.008 kb on - strand at 5679.133 kb on - strand at 5679.133 kb on - strand at 5679.233 kb on + strand at 5679.603 kb on + strand at 5679.759 kb on - strand, within Pf1N1B4_5119 at 5680.130 kb on + strand at 5681.058 kb on + strand, within Pf1N1B4_5119 at 5681.059 kb on - strand, within Pf1N1B4_5119 at 5681.296 kb on + strand, within Pf1N1B4_5119 at 5681.297 kb on - strand, within Pf1N1B4_5119 at 5681.392 kb on + strand, within Pf1N1B4_5119 at 5681.538 kb on + strand, within Pf1N1B4_5119 at 5681.539 kb on - strand, within Pf1N1B4_5119 at 5681.539 kb on - strand, within Pf1N1B4_5119 at 5681.539 kb on - strand, within Pf1N1B4_5119 at 5681.541 kb on + strand, within Pf1N1B4_5119 at 5681.928 kb on + strand at 5682.321 kb on + strand at 5682.321 kb on + strand at 5682.328 kb on - strand at 5682.542 kb on + strand at 5683.121 kb on + strand, within Pf1N1B4_5121
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 5,678,709 - +0.3 5,678,709 - Pf1N1B4_5118 0.56 -0.9 5,678,868 + -1.7 5,678,869 - +0.3 5,678,892 + +0.3 5,678,895 + -0.6 5,679,007 + -0.7 5,679,008 - +0.3 5,679,008 - -0.4 5,679,008 - +0.3 5,679,133 - -1.0 5,679,133 - +1.7 5,679,233 + +0.3 5,679,603 + -0.7 5,679,759 - Pf1N1B4_5119 0.13 +1.6 5,680,130 + -1.7 5,681,058 + Pf1N1B4_5119 0.61 -0.7 5,681,059 - Pf1N1B4_5119 0.61 -0.5 5,681,296 + Pf1N1B4_5119 0.69 -1.0 5,681,297 - Pf1N1B4_5119 0.69 -0.5 5,681,392 + Pf1N1B4_5119 0.73 -1.0 5,681,538 + Pf1N1B4_5119 0.78 -1.6 5,681,539 - Pf1N1B4_5119 0.78 +0.6 5,681,539 - Pf1N1B4_5119 0.78 -1.6 5,681,539 - Pf1N1B4_5119 0.78 +0.3 5,681,541 + Pf1N1B4_5119 0.78 -0.4 5,681,928 + -0.3 5,682,321 + +0.3 5,682,321 + -0.1 5,682,328 - -0.6 5,682,542 + -0.6 5,683,121 + Pf1N1B4_5121 0.13 +2.5
Or see this region's nucleotide sequence