Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5070 and Pf1N1B4_5071 are separated by 233 nucleotides Pf1N1B4_5071 and Pf1N1B4_5072 are separated by 90 nucleotides Pf1N1B4_5072 and Pf1N1B4_5073 are separated by 4 nucleotides
Pf1N1B4_5070 - Aspartate aminotransferase (EC 2.6.1.1), at 5,615,002 to 5,616,216
_5070
Pf1N1B4_5071 - FIG00959535: hypothetical protein, at 5,616,450 to 5,617,673
_5071
Pf1N1B4_5072 - Glycerol-3-phosphate regulon repressor, at 5,617,764 to 5,618,582
_5072
Pf1N1B4_5073 - Hydroxypyruvate isomerase (EC 5.3.1.22), at 5,618,587 to 5,619,369
_5073
Position (kb) 5616
5617
5618 Strain fitness (log2 ratio) -1
0
1
2 at 5615.555 kb on - strand, within Pf1N1B4_5070 at 5615.632 kb on + strand at 5615.632 kb on + strand, within Pf1N1B4_5070 at 5615.632 kb on + strand, within Pf1N1B4_5070 at 5615.632 kb on + strand, within Pf1N1B4_5070 at 5615.633 kb on - strand, within Pf1N1B4_5070 at 5615.633 kb on - strand, within Pf1N1B4_5070 at 5615.633 kb on - strand, within Pf1N1B4_5070 at 5615.633 kb on - strand, within Pf1N1B4_5070 at 5615.633 kb on - strand, within Pf1N1B4_5070 at 5615.633 kb on - strand, within Pf1N1B4_5070 at 5615.666 kb on - strand at 5615.666 kb on - strand, within Pf1N1B4_5070 at 5615.986 kb on + strand, within Pf1N1B4_5070 at 5615.986 kb on + strand at 5615.987 kb on - strand, within Pf1N1B4_5070 at 5616.201 kb on + strand at 5616.222 kb on - strand at 5616.296 kb on + strand at 5616.297 kb on - strand at 5616.310 kb on - strand at 5616.345 kb on - strand at 5617.002 kb on + strand at 5617.324 kb on - strand, within Pf1N1B4_5071 at 5617.521 kb on + strand, within Pf1N1B4_5071 at 5617.521 kb on + strand, within Pf1N1B4_5071 at 5617.549 kb on - strand, within Pf1N1B4_5071 at 5617.711 kb on + strand at 5617.954 kb on - strand, within Pf1N1B4_5072 at 5618.094 kb on + strand, within Pf1N1B4_5072 at 5618.133 kb on + strand, within Pf1N1B4_5072 at 5618.136 kb on - strand, within Pf1N1B4_5072 at 5618.457 kb on + strand, within Pf1N1B4_5072 at 5618.458 kb on - strand, within Pf1N1B4_5072 at 5618.580 kb on + strand at 5618.581 kb on - strand at 5618.635 kb on - strand at 5618.673 kb on - strand, within Pf1N1B4_5073
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 5,615,555 - Pf1N1B4_5070 0.46 -0.6 5,615,632 + +0.7 5,615,632 + Pf1N1B4_5070 0.52 +0.4 5,615,632 + Pf1N1B4_5070 0.52 +0.3 5,615,632 + Pf1N1B4_5070 0.52 +0.7 5,615,633 - Pf1N1B4_5070 0.52 +0.7 5,615,633 - Pf1N1B4_5070 0.52 +0.3 5,615,633 - Pf1N1B4_5070 0.52 -0.0 5,615,633 - Pf1N1B4_5070 0.52 -1.6 5,615,633 - Pf1N1B4_5070 0.52 -0.4 5,615,633 - Pf1N1B4_5070 0.52 -0.5 5,615,666 - +0.7 5,615,666 - Pf1N1B4_5070 0.55 -1.1 5,615,986 + Pf1N1B4_5070 0.81 +0.3 5,615,986 + +0.3 5,615,987 - Pf1N1B4_5070 0.81 +1.1 5,616,201 + -1.0 5,616,222 - +0.5 5,616,296 + +1.8 5,616,297 - -0.7 5,616,310 - -0.2 5,616,345 - -0.1 5,617,002 + -0.3 5,617,324 - Pf1N1B4_5071 0.71 -0.6 5,617,521 + Pf1N1B4_5071 0.88 -0.6 5,617,521 + Pf1N1B4_5071 0.88 -0.6 5,617,549 - Pf1N1B4_5071 0.90 -0.8 5,617,711 + -0.2 5,617,954 - Pf1N1B4_5072 0.23 +1.3 5,618,094 + Pf1N1B4_5072 0.40 -0.4 5,618,133 + Pf1N1B4_5072 0.45 +1.1 5,618,136 - Pf1N1B4_5072 0.45 +2.4 5,618,457 + Pf1N1B4_5072 0.85 -0.6 5,618,458 - Pf1N1B4_5072 0.85 +0.1 5,618,580 + +1.4 5,618,581 - -0.3 5,618,635 - -1.0 5,618,673 - Pf1N1B4_5073 0.11 +0.5
Or see this region's nucleotide sequence