Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5009 and Pf1N1B4_5010 are separated by 185 nucleotides Pf1N1B4_5010 and Pf1N1B4_5011 are separated by 125 nucleotides Pf1N1B4_5011 and Pf1N1B4_5012 are separated by 137 nucleotides
Pf1N1B4_5009 - Lactoylglutathione lyase (EC 4.4.1.5), at 5,559,879 to 5,560,400
_5009
Pf1N1B4_5010 - Alkyl hydroperoxide reductase protein F (EC 1.6.4.-), at 5,560,586 to 5,562,148
_5010
Pf1N1B4_5011 - Alkyl hydroperoxide reductase protein C (EC 1.6.4.-), at 5,562,274 to 5,562,837
_5011
Pf1N1B4_5012 - Cointegrate resolution protein S, at 5,562,975 to 5,563,970
_5012
Position (kb)
5560
5561
5562
5563 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5559.680 kb on - strand at 5559.841 kb on + strand at 5559.842 kb on - strand at 5559.899 kb on + strand at 5560.068 kb on + strand at 5560.238 kb on + strand, within Pf1N1B4_5009 at 5560.398 kb on + strand at 5560.398 kb on + strand at 5560.441 kb on + strand at 5560.587 kb on + strand at 5560.673 kb on - strand at 5560.673 kb on - strand at 5560.937 kb on - strand, within Pf1N1B4_5010 at 5560.992 kb on - strand at 5561.124 kb on + strand, within Pf1N1B4_5010 at 5561.141 kb on - strand, within Pf1N1B4_5010 at 5561.453 kb on - strand, within Pf1N1B4_5010 at 5561.463 kb on + strand, within Pf1N1B4_5010 at 5561.464 kb on - strand, within Pf1N1B4_5010 at 5561.508 kb on - strand, within Pf1N1B4_5010 at 5561.508 kb on - strand, within Pf1N1B4_5010 at 5561.628 kb on + strand, within Pf1N1B4_5010 at 5561.629 kb on - strand, within Pf1N1B4_5010 at 5561.781 kb on + strand, within Pf1N1B4_5010 at 5561.782 kb on - strand at 5561.782 kb on - strand, within Pf1N1B4_5010 at 5561.782 kb on - strand, within Pf1N1B4_5010 at 5561.782 kb on - strand, within Pf1N1B4_5010 at 5561.998 kb on + strand at 5561.998 kb on + strand at 5562.148 kb on + strand at 5562.148 kb on + strand at 5562.148 kb on + strand at 5562.229 kb on - strand at 5562.229 kb on - strand at 5562.232 kb on + strand at 5562.232 kb on + strand at 5562.276 kb on - strand at 5562.276 kb on - strand at 5562.276 kb on - strand at 5562.416 kb on - strand, within Pf1N1B4_5011 at 5562.813 kb on + strand at 5562.882 kb on - strand at 5562.956 kb on + strand at 5562.960 kb on - strand at 5563.036 kb on + strand at 5563.037 kb on - strand at 5563.037 kb on - strand at 5563.037 kb on - strand at 5563.072 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 5,559,680 - +1.5 5,559,841 + +1.6 5,559,842 - +0.3 5,559,899 + +0.3 5,560,068 + -0.2 5,560,238 + Pf1N1B4_5009 0.69 +1.7 5,560,398 + +1.4 5,560,398 + +0.5 5,560,441 + +0.1 5,560,587 + -0.1 5,560,673 - -0.5 5,560,673 - +0.7 5,560,937 - Pf1N1B4_5010 0.22 -0.3 5,560,992 - -1.3 5,561,124 + Pf1N1B4_5010 0.34 -1.0 5,561,141 - Pf1N1B4_5010 0.36 -1.3 5,561,453 - Pf1N1B4_5010 0.55 +0.8 5,561,463 + Pf1N1B4_5010 0.56 -0.0 5,561,464 - Pf1N1B4_5010 0.56 -0.7 5,561,508 - Pf1N1B4_5010 0.59 -0.2 5,561,508 - Pf1N1B4_5010 0.59 -0.7 5,561,628 + Pf1N1B4_5010 0.67 -0.5 5,561,629 - Pf1N1B4_5010 0.67 -0.7 5,561,781 + Pf1N1B4_5010 0.76 -2.6 5,561,782 - -1.3 5,561,782 - Pf1N1B4_5010 0.77 -0.7 5,561,782 - Pf1N1B4_5010 0.77 -1.0 5,561,782 - Pf1N1B4_5010 0.77 -0.6 5,561,998 + -1.5 5,561,998 + -0.4 5,562,148 + -2.2 5,562,148 + +0.2 5,562,148 + +1.2 5,562,229 - -1.0 5,562,229 - -0.2 5,562,232 + -0.3 5,562,232 + +1.8 5,562,276 - -0.9 5,562,276 - +0.3 5,562,276 - +0.6 5,562,416 - Pf1N1B4_5011 0.25 +0.8 5,562,813 + -0.7 5,562,882 - +0.3 5,562,956 + +1.4 5,562,960 - +0.9 5,563,036 + -1.2 5,563,037 - +0.3 5,563,037 - -1.2 5,563,037 - +0.4 5,563,072 + -1.7
Or see this region's nucleotide sequence