Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4978 and Pf1N1B4_4979 are separated by 120 nucleotides Pf1N1B4_4979 and Pf1N1B4_4980 are separated by 197 nucleotides
Pf1N1B4_4978 - Deacetylases, including yeast histone deacetylase and acetoin utilization protein, at 5,517,901 to 5,518,935
_4978
Pf1N1B4_4979 - Transcriptional regulator, AsnC family, at 5,519,056 to 5,519,556
_4979
Pf1N1B4_4980 - putative lipoprotein, at 5,519,754 to 5,520,896
_4980
Position (kb)
5519
5520 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5518.102 kb on - strand, within Pf1N1B4_4978 at 5518.106 kb on - strand, within Pf1N1B4_4978 at 5518.163 kb on + strand, within Pf1N1B4_4978 at 5518.208 kb on + strand, within Pf1N1B4_4978 at 5518.253 kb on + strand at 5518.256 kb on + strand, within Pf1N1B4_4978 at 5518.316 kb on + strand, within Pf1N1B4_4978 at 5518.316 kb on + strand, within Pf1N1B4_4978 at 5518.377 kb on + strand, within Pf1N1B4_4978 at 5518.427 kb on + strand at 5518.445 kb on + strand, within Pf1N1B4_4978 at 5518.445 kb on + strand, within Pf1N1B4_4978 at 5518.445 kb on + strand, within Pf1N1B4_4978 at 5518.445 kb on + strand, within Pf1N1B4_4978 at 5518.739 kb on + strand, within Pf1N1B4_4978 at 5518.886 kb on - strand at 5518.938 kb on - strand at 5518.938 kb on - strand at 5518.940 kb on - strand at 5518.970 kb on - strand at 5519.289 kb on + strand, within Pf1N1B4_4979 at 5519.289 kb on + strand, within Pf1N1B4_4979 at 5519.409 kb on - strand, within Pf1N1B4_4979 at 5519.431 kb on + strand, within Pf1N1B4_4979 at 5519.432 kb on - strand, within Pf1N1B4_4979 at 5519.432 kb on - strand, within Pf1N1B4_4979 at 5519.432 kb on - strand, within Pf1N1B4_4979 at 5519.453 kb on + strand, within Pf1N1B4_4979 at 5519.481 kb on + strand, within Pf1N1B4_4979 at 5520.135 kb on - strand, within Pf1N1B4_4980 at 5520.194 kb on + strand, within Pf1N1B4_4980 at 5520.428 kb on + strand at 5520.428 kb on + strand, within Pf1N1B4_4980
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 5,518,102 - Pf1N1B4_4978 0.19 +0.5 5,518,106 - Pf1N1B4_4978 0.20 -0.3 5,518,163 + Pf1N1B4_4978 0.25 +0.7 5,518,208 + Pf1N1B4_4978 0.30 -1.6 5,518,253 + -2.1 5,518,256 + Pf1N1B4_4978 0.34 -2.3 5,518,316 + Pf1N1B4_4978 0.40 -0.2 5,518,316 + Pf1N1B4_4978 0.40 -0.7 5,518,377 + Pf1N1B4_4978 0.46 -0.3 5,518,427 + -0.3 5,518,445 + Pf1N1B4_4978 0.53 +0.7 5,518,445 + Pf1N1B4_4978 0.53 -0.3 5,518,445 + Pf1N1B4_4978 0.53 -0.6 5,518,445 + Pf1N1B4_4978 0.53 -0.3 5,518,739 + Pf1N1B4_4978 0.81 -0.1 5,518,886 - +0.3 5,518,938 - +0.1 5,518,938 - -2.1 5,518,940 - +0.3 5,518,970 - +0.3 5,519,289 + Pf1N1B4_4979 0.47 +0.3 5,519,289 + Pf1N1B4_4979 0.47 +1.6 5,519,409 - Pf1N1B4_4979 0.70 -0.7 5,519,431 + Pf1N1B4_4979 0.75 -0.3 5,519,432 - Pf1N1B4_4979 0.75 -0.6 5,519,432 - Pf1N1B4_4979 0.75 -2.1 5,519,432 - Pf1N1B4_4979 0.75 -0.2 5,519,453 + Pf1N1B4_4979 0.79 -0.2 5,519,481 + Pf1N1B4_4979 0.85 -0.9 5,520,135 - Pf1N1B4_4980 0.33 -1.2 5,520,194 + Pf1N1B4_4980 0.38 +0.3 5,520,428 + +0.3 5,520,428 + Pf1N1B4_4980 0.59 +2.3
Or see this region's nucleotide sequence