Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4931 and Pf1N1B4_4932 are separated by 40 nucleotides Pf1N1B4_4932 and Pf1N1B4_4933 are separated by 171 nucleotides Pf1N1B4_4933 and Pf1N1B4_4934 are separated by 224 nucleotides
Pf1N1B4_4931 - Glutarate-semialdehyde dehydrogenase (EC 1.2.1.20); Succinate-semialdehyde dehydrogenase [NADP+] (EC 1.2.1.79), at 5,465,202 to 5,466,659
_4931
Pf1N1B4_4932 - Tartrate dehydrogenase (EC 1.1.1.93) / Tartrate decarboxylase (EC 4.1.1.73) / D-malic enzyme (EC 1.1.1.83), at 5,466,700 to 5,467,779
_4932
Pf1N1B4_4933 - FIG00955097: hypothetical protein, at 5,467,951 to 5,468,175
_4933
Pf1N1B4_4934 - DNA damage response nuclease (from data) , at 5,468,400 to 5,470,052
_4934
Position (kb)
5466
5467
5468 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5466.114 kb on + strand, within Pf1N1B4_4931 at 5466.115 kb on - strand, within Pf1N1B4_4931 at 5466.457 kb on - strand at 5466.460 kb on - strand, within Pf1N1B4_4931 at 5466.460 kb on - strand, within Pf1N1B4_4931 at 5466.606 kb on + strand at 5466.622 kb on - strand at 5466.646 kb on + strand at 5466.647 kb on - strand at 5466.647 kb on - strand at 5466.824 kb on + strand, within Pf1N1B4_4932 at 5466.847 kb on + strand at 5467.006 kb on + strand, within Pf1N1B4_4932 at 5467.117 kb on + strand, within Pf1N1B4_4932 at 5467.567 kb on + strand, within Pf1N1B4_4932 at 5467.567 kb on + strand, within Pf1N1B4_4932 at 5467.568 kb on - strand, within Pf1N1B4_4932 at 5467.926 kb on - strand at 5468.072 kb on + strand at 5468.175 kb on + strand at 5468.176 kb on - strand at 5468.347 kb on - strand at 5468.347 kb on - strand at 5468.347 kb on - strand at 5468.353 kb on + strand at 5468.408 kb on - strand at 5468.408 kb on - strand at 5468.488 kb on + strand at 5468.610 kb on + strand, within Pf1N1B4_4934 at 5468.611 kb on - strand, within Pf1N1B4_4934
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 5,466,114 + Pf1N1B4_4931 0.63 -0.9 5,466,115 - Pf1N1B4_4931 0.63 +0.3 5,466,457 - +1.6 5,466,460 - Pf1N1B4_4931 0.86 -1.3 5,466,460 - Pf1N1B4_4931 0.86 -0.2 5,466,606 + +0.7 5,466,622 - -0.6 5,466,646 + -1.6 5,466,647 - -0.8 5,466,647 - +1.1 5,466,824 + Pf1N1B4_4932 0.11 -0.4 5,466,847 + +1.3 5,467,006 + Pf1N1B4_4932 0.28 +0.3 5,467,117 + Pf1N1B4_4932 0.39 -1.3 5,467,567 + Pf1N1B4_4932 0.80 -1.6 5,467,567 + Pf1N1B4_4932 0.80 -1.3 5,467,568 - Pf1N1B4_4932 0.80 -0.4 5,467,926 - -1.3 5,468,072 + -0.7 5,468,175 + -0.3 5,468,176 - -0.6 5,468,347 - -0.5 5,468,347 - +2.3 5,468,347 - +0.3 5,468,353 + +0.7 5,468,408 - +1.3 5,468,408 - +0.7 5,468,488 + -0.0 5,468,610 + Pf1N1B4_4934 0.13 -0.5 5,468,611 - Pf1N1B4_4934 0.13 -2.9
Or see this region's nucleotide sequence