Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4770 and Pf1N1B4_4771 are separated by 101 nucleotides Pf1N1B4_4771 and Pf1N1B4_4772 are separated by 190 nucleotides Pf1N1B4_4772 and Pf1N1B4_4773 are separated by 15 nucleotides
Pf1N1B4_4770 - Putative autotransporter, at 5,268,195 to 5,270,408
_4770
Pf1N1B4_4771 - Probable transmembrane protein, at 5,270,510 to 5,271,151
_4771
Pf1N1B4_4772 - RND efflux system, inner membrane transporter CmeB, at 5,271,342 to 5,274,440
_4772
Pf1N1B4_4773 - RND efflux system, membrane fusion protein CmeA, at 5,274,456 to 5,275,613
_4773
Position (kb) 5271
5272
5273
5274
5275 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 5270.386 kb on + strand at 5270.386 kb on + strand at 5270.386 kb on + strand at 5270.386 kb on + strand at 5270.397 kb on + strand at 5270.398 kb on - strand at 5270.561 kb on - strand at 5270.561 kb on - strand at 5270.561 kb on - strand at 5270.561 kb on - strand at 5270.561 kb on - strand at 5270.745 kb on + strand, within Pf1N1B4_4771 at 5270.745 kb on + strand at 5270.746 kb on - strand, within Pf1N1B4_4771 at 5270.748 kb on + strand, within Pf1N1B4_4771 at 5270.748 kb on + strand at 5270.749 kb on - strand, within Pf1N1B4_4771 at 5270.749 kb on - strand at 5270.760 kb on + strand, within Pf1N1B4_4771 at 5270.761 kb on - strand, within Pf1N1B4_4771 at 5270.927 kb on + strand at 5270.927 kb on + strand at 5270.927 kb on + strand, within Pf1N1B4_4771 at 5271.177 kb on - strand at 5271.602 kb on - strand at 5271.894 kb on + strand, within Pf1N1B4_4772 at 5272.083 kb on - strand, within Pf1N1B4_4772 at 5272.083 kb on - strand, within Pf1N1B4_4772 at 5272.083 kb on - strand, within Pf1N1B4_4772 at 5272.083 kb on - strand, within Pf1N1B4_4772 at 5272.087 kb on - strand at 5272.087 kb on - strand, within Pf1N1B4_4772 at 5272.187 kb on - strand, within Pf1N1B4_4772 at 5273.109 kb on - strand, within Pf1N1B4_4772 at 5273.419 kb on + strand at 5273.419 kb on + strand, within Pf1N1B4_4772 at 5273.476 kb on + strand, within Pf1N1B4_4772 at 5273.477 kb on - strand, within Pf1N1B4_4772 at 5273.745 kb on - strand, within Pf1N1B4_4772 at 5273.988 kb on + strand, within Pf1N1B4_4772
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 5,270,386 + -1.0 5,270,386 + +0.1 5,270,386 + +0.4 5,270,386 + +0.3 5,270,397 + -0.3 5,270,398 - -0.4 5,270,561 - -2.1 5,270,561 - -0.2 5,270,561 - -0.3 5,270,561 - +0.4 5,270,561 - +0.3 5,270,745 + Pf1N1B4_4771 0.37 -0.3 5,270,745 + +0.3 5,270,746 - Pf1N1B4_4771 0.37 +0.9 5,270,748 + Pf1N1B4_4771 0.37 -0.2 5,270,748 + +1.3 5,270,749 - Pf1N1B4_4771 0.37 -2.3 5,270,749 - +0.3 5,270,760 + Pf1N1B4_4771 0.39 +0.7 5,270,761 - Pf1N1B4_4771 0.39 +0.3 5,270,927 + +0.3 5,270,927 + +2.6 5,270,927 + Pf1N1B4_4771 0.65 +0.2 5,271,177 - -2.1 5,271,602 - -2.7 5,271,894 + Pf1N1B4_4772 0.18 -1.6 5,272,083 - Pf1N1B4_4772 0.24 -5.2 5,272,083 - Pf1N1B4_4772 0.24 -1.3 5,272,083 - Pf1N1B4_4772 0.24 -0.3 5,272,083 - Pf1N1B4_4772 0.24 -1.9 5,272,087 - -0.7 5,272,087 - Pf1N1B4_4772 0.24 -0.1 5,272,187 - Pf1N1B4_4772 0.27 -2.1 5,273,109 - Pf1N1B4_4772 0.57 -2.6 5,273,419 + +0.3 5,273,419 + Pf1N1B4_4772 0.67 -1.3 5,273,476 + Pf1N1B4_4772 0.69 -3.3 5,273,477 - Pf1N1B4_4772 0.69 -3.3 5,273,745 - Pf1N1B4_4772 0.78 -2.6 5,273,988 + Pf1N1B4_4772 0.85 -3.1
Or see this region's nucleotide sequence