Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4754 and Pf1N1B4_4755 are separated by 201 nucleotides Pf1N1B4_4755 and Pf1N1B4_4756 are separated by 7 nucleotides
Pf1N1B4_4754 - GNAT family acetyltransferase VC2332, at 5,251,694 to 5,252,791
_4754
Pf1N1B4_4755 - ribosomal protein S6 glutaminyl transferase related protein, at 5,252,993 to 5,254,471
_4755
Pf1N1B4_4756 - Magnesium and cobalt transport protein CorA, at 5,254,479 to 5,255,402
_4756
Position (kb) 5252
5253
5254
5255 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5252.028 kb on - strand, within Pf1N1B4_4754 at 5252.028 kb on - strand, within Pf1N1B4_4754 at 5252.040 kb on - strand, within Pf1N1B4_4754 at 5252.102 kb on + strand at 5252.103 kb on - strand, within Pf1N1B4_4754 at 5252.311 kb on - strand, within Pf1N1B4_4754 at 5252.392 kb on - strand, within Pf1N1B4_4754 at 5252.434 kb on - strand at 5252.578 kb on - strand, within Pf1N1B4_4754 at 5252.792 kb on - strand at 5252.885 kb on - strand at 5253.010 kb on + strand at 5253.030 kb on - strand at 5253.132 kb on - strand at 5253.138 kb on - strand at 5253.138 kb on - strand at 5253.153 kb on - strand, within Pf1N1B4_4755 at 5253.156 kb on - strand, within Pf1N1B4_4755 at 5253.453 kb on + strand, within Pf1N1B4_4755 at 5254.145 kb on + strand, within Pf1N1B4_4755 at 5254.146 kb on - strand, within Pf1N1B4_4755 at 5254.175 kb on + strand, within Pf1N1B4_4755 at 5254.176 kb on - strand, within Pf1N1B4_4755 at 5254.299 kb on - strand, within Pf1N1B4_4755 at 5254.388 kb on + strand at 5254.388 kb on + strand at 5254.389 kb on - strand at 5254.389 kb on - strand at 5254.406 kb on + strand at 5254.406 kb on + strand at 5254.406 kb on + strand at 5254.704 kb on + strand, within Pf1N1B4_4756 at 5254.704 kb on + strand, within Pf1N1B4_4756 at 5254.871 kb on + strand, within Pf1N1B4_4756 at 5254.881 kb on + strand at 5254.963 kb on + strand, within Pf1N1B4_4756 at 5254.964 kb on - strand, within Pf1N1B4_4756 at 5254.998 kb on + strand, within Pf1N1B4_4756 at 5254.998 kb on + strand, within Pf1N1B4_4756 at 5254.998 kb on + strand, within Pf1N1B4_4756 at 5254.998 kb on + strand at 5254.998 kb on + strand, within Pf1N1B4_4756 at 5254.998 kb on + strand, within Pf1N1B4_4756 at 5254.999 kb on - strand, within Pf1N1B4_4756 at 5254.999 kb on - strand at 5255.001 kb on + strand, within Pf1N1B4_4756 at 5255.002 kb on - strand, within Pf1N1B4_4756 at 5255.002 kb on - strand, within Pf1N1B4_4756 at 5255.127 kb on + strand, within Pf1N1B4_4756 at 5255.128 kb on - strand, within Pf1N1B4_4756 at 5255.145 kb on + strand, within Pf1N1B4_4756 at 5255.450 kb on + strand at 5255.450 kb on + strand at 5255.450 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 5,252,028 - Pf1N1B4_4754 0.30 +1.5 5,252,028 - Pf1N1B4_4754 0.30 -1.3 5,252,040 - Pf1N1B4_4754 0.32 +1.9 5,252,102 + +0.9 5,252,103 - Pf1N1B4_4754 0.37 -1.2 5,252,311 - Pf1N1B4_4754 0.56 -0.2 5,252,392 - Pf1N1B4_4754 0.64 -0.2 5,252,434 - -0.7 5,252,578 - Pf1N1B4_4754 0.81 -0.5 5,252,792 - +0.3 5,252,885 - +1.0 5,253,010 + -0.6 5,253,030 - +0.0 5,253,132 - +0.7 5,253,138 - -1.7 5,253,138 - +0.3 5,253,153 - Pf1N1B4_4755 0.11 +0.2 5,253,156 - Pf1N1B4_4755 0.11 -0.7 5,253,453 + Pf1N1B4_4755 0.31 -1.7 5,254,145 + Pf1N1B4_4755 0.78 +0.5 5,254,146 - Pf1N1B4_4755 0.78 -0.3 5,254,175 + Pf1N1B4_4755 0.80 -1.7 5,254,176 - Pf1N1B4_4755 0.80 -0.3 5,254,299 - Pf1N1B4_4755 0.88 -1.3 5,254,388 + -0.3 5,254,388 + -2.5 5,254,389 - +0.1 5,254,389 - -1.7 5,254,406 + +0.8 5,254,406 + +1.9 5,254,406 + -0.1 5,254,704 + Pf1N1B4_4756 0.24 -2.3 5,254,704 + Pf1N1B4_4756 0.24 -0.8 5,254,871 + Pf1N1B4_4756 0.42 +0.8 5,254,881 + -0.7 5,254,963 + Pf1N1B4_4756 0.52 -0.3 5,254,964 - Pf1N1B4_4756 0.52 +1.0 5,254,998 + Pf1N1B4_4756 0.56 +0.3 5,254,998 + Pf1N1B4_4756 0.56 +1.0 5,254,998 + Pf1N1B4_4756 0.56 +0.5 5,254,998 + -1.3 5,254,998 + Pf1N1B4_4756 0.56 +0.1 5,254,998 + Pf1N1B4_4756 0.56 -0.6 5,254,999 - Pf1N1B4_4756 0.56 +0.3 5,254,999 - +1.3 5,255,001 + Pf1N1B4_4756 0.56 +0.3 5,255,002 - Pf1N1B4_4756 0.57 -0.0 5,255,002 - Pf1N1B4_4756 0.57 -0.2 5,255,127 + Pf1N1B4_4756 0.70 +0.3 5,255,128 - Pf1N1B4_4756 0.70 -0.2 5,255,145 + Pf1N1B4_4756 0.72 -2.0 5,255,450 + -0.2 5,255,450 + +0.6 5,255,450 + +1.1
Or see this region's nucleotide sequence