Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4729 and Pf1N1B4_4730 are separated by 282 nucleotides Pf1N1B4_4730 and Pf1N1B4_4731 are separated by 342 nucleotides Pf1N1B4_4731 and Pf1N1B4_4732 are separated by 126 nucleotides
Pf1N1B4_4729 - Hemolysin-type calcium-binding region, at 5,226,195 to 5,227,484
_4729
Pf1N1B4_4730 - FIG00960186: hypothetical protein, at 5,227,767 to 5,228,030
_4730
Pf1N1B4_4731 - Sigma-54 dependent transcriptional regulator, at 5,228,373 to 5,229,698
_4731
Pf1N1B4_4732 - Fap amyloid fiber secretin, at 5,229,825 to 5,230,994
_4732
Position (kb) 5228
5229
5230 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5227.653 kb on - strand at 5227.704 kb on + strand at 5227.704 kb on + strand at 5227.719 kb on + strand at 5227.719 kb on + strand at 5227.720 kb on - strand at 5227.768 kb on + strand at 5227.799 kb on - strand, within Pf1N1B4_4730 at 5227.930 kb on - strand, within Pf1N1B4_4730 at 5228.045 kb on - strand at 5228.045 kb on - strand at 5228.047 kb on - strand at 5228.047 kb on - strand at 5228.103 kb on - strand at 5228.242 kb on - strand at 5228.248 kb on - strand at 5228.267 kb on - strand at 5228.480 kb on + strand at 5228.480 kb on + strand at 5228.481 kb on - strand at 5229.259 kb on - strand, within Pf1N1B4_4731 at 5229.259 kb on - strand, within Pf1N1B4_4731 at 5229.259 kb on - strand, within Pf1N1B4_4731 at 5229.259 kb on - strand, within Pf1N1B4_4731 at 5229.351 kb on + strand, within Pf1N1B4_4731 at 5229.352 kb on - strand, within Pf1N1B4_4731 at 5229.352 kb on - strand, within Pf1N1B4_4731 at 5229.598 kb on + strand at 5229.598 kb on + strand at 5229.598 kb on + strand at 5229.598 kb on + strand at 5229.599 kb on - strand at 5229.603 kb on - strand at 5229.644 kb on - strand at 5229.659 kb on + strand at 5229.666 kb on + strand at 5229.666 kb on + strand at 5229.666 kb on + strand at 5229.814 kb on + strand at 5229.815 kb on - strand at 5229.815 kb on - strand at 5229.832 kb on + strand at 5229.833 kb on - strand at 5229.833 kb on - strand at 5230.049 kb on + strand, within Pf1N1B4_4732 at 5230.177 kb on + strand at 5230.177 kb on + strand at 5230.177 kb on + strand, within Pf1N1B4_4732 at 5230.183 kb on + strand, within Pf1N1B4_4732 at 5230.184 kb on - strand at 5230.184 kb on - strand, within Pf1N1B4_4732 at 5230.411 kb on + strand, within Pf1N1B4_4732 at 5230.507 kb on + strand, within Pf1N1B4_4732 at 5230.507 kb on + strand at 5230.687 kb on + strand, within Pf1N1B4_4732 at 5230.688 kb on - strand, within Pf1N1B4_4732 at 5230.696 kb on + strand, within Pf1N1B4_4732 at 5230.696 kb on + strand, within Pf1N1B4_4732
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 5,227,653 - +0.8 5,227,704 + +1.4 5,227,704 + +0.5 5,227,719 + -1.6 5,227,719 + +0.1 5,227,720 - +1.7 5,227,768 + -0.1 5,227,799 - Pf1N1B4_4730 0.12 +1.3 5,227,930 - Pf1N1B4_4730 0.62 -0.4 5,228,045 - +0.1 5,228,045 - -0.7 5,228,047 - -1.5 5,228,047 - +0.6 5,228,103 - +0.9 5,228,242 - +0.5 5,228,248 - +0.0 5,228,267 - -1.1 5,228,480 + -1.4 5,228,480 + +2.6 5,228,481 - -0.1 5,229,259 - Pf1N1B4_4731 0.67 -2.7 5,229,259 - Pf1N1B4_4731 0.67 -0.9 5,229,259 - Pf1N1B4_4731 0.67 -2.9 5,229,259 - Pf1N1B4_4731 0.67 -0.7 5,229,351 + Pf1N1B4_4731 0.74 -0.7 5,229,352 - Pf1N1B4_4731 0.74 -0.7 5,229,352 - Pf1N1B4_4731 0.74 -0.0 5,229,598 + -0.4 5,229,598 + -0.3 5,229,598 + +0.0 5,229,598 + -1.0 5,229,599 - +0.5 5,229,603 - +1.0 5,229,644 - -0.1 5,229,659 + -0.9 5,229,666 + +1.5 5,229,666 + +0.9 5,229,666 + -0.3 5,229,814 + -0.9 5,229,815 - -0.7 5,229,815 - +0.7 5,229,832 + +0.3 5,229,833 - +0.3 5,229,833 - +0.3 5,230,049 + Pf1N1B4_4732 0.19 -2.3 5,230,177 + +0.7 5,230,177 + +0.7 5,230,177 + Pf1N1B4_4732 0.30 +0.1 5,230,183 + Pf1N1B4_4732 0.31 -0.1 5,230,184 - -0.7 5,230,184 - Pf1N1B4_4732 0.31 -0.4 5,230,411 + Pf1N1B4_4732 0.50 +0.4 5,230,507 + Pf1N1B4_4732 0.58 +0.3 5,230,507 + +1.6 5,230,687 + Pf1N1B4_4732 0.74 -0.5 5,230,688 - Pf1N1B4_4732 0.74 -0.8 5,230,696 + Pf1N1B4_4732 0.74 -1.0 5,230,696 + Pf1N1B4_4732 0.74 -0.2
Or see this region's nucleotide sequence