Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4591

Experiment: Spectinomycin 0.025 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4590 and Pf1N1B4_4591 are separated by 1 nucleotidesPf1N1B4_4591 and Pf1N1B4_4592 are separated by 1 nucleotides Pf1N1B4_4590 - Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1), at 5,060,449 to 5,061,900 _4590 Pf1N1B4_4591 - Oligopeptide transport system permease protein OppC (TC 3.A.1.5.1), at 5,061,902 to 5,062,921 _4591 Pf1N1B4_4592 - Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1), at 5,062,923 to 5,063,996 _4592 Position (kb) 5061 5062 5063Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 5061.007 kb on - strand, within Pf1N1B4_4590at 5061.239 kb on - strand, within Pf1N1B4_4590at 5061.239 kb on - strand, within Pf1N1B4_4590at 5061.293 kb on - strand, within Pf1N1B4_4590at 5061.681 kb on - strand, within Pf1N1B4_4590at 5061.681 kb on - strand, within Pf1N1B4_4590at 5062.221 kb on + strand, within Pf1N1B4_4591at 5062.603 kb on - strandat 5062.970 kb on - strandat 5063.249 kb on - strand, within Pf1N1B4_4592at 5063.354 kb on - strand, within Pf1N1B4_4592at 5063.746 kb on + strand, within Pf1N1B4_4592at 5063.747 kb on - strand, within Pf1N1B4_4592

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Per-strain Table

Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml
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5,061,007 - Pf1N1B4_4590 0.38 -1.0
5,061,239 - Pf1N1B4_4590 0.54 -0.4
5,061,239 - Pf1N1B4_4590 0.54 -2.5
5,061,293 - Pf1N1B4_4590 0.58 -0.3
5,061,681 - Pf1N1B4_4590 0.85 -0.8
5,061,681 - Pf1N1B4_4590 0.85 +0.3
5,062,221 + Pf1N1B4_4591 0.31 -1.5
5,062,603 - +1.7
5,062,970 - -0.3
5,063,249 - Pf1N1B4_4592 0.30 +0.3
5,063,354 - Pf1N1B4_4592 0.40 +2.1
5,063,746 + Pf1N1B4_4592 0.77 -3.4
5,063,747 - Pf1N1B4_4592 0.77 +0.8

Or see this region's nucleotide sequence