Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_455 and Pf1N1B4_456 are separated by 104 nucleotides Pf1N1B4_456 and Pf1N1B4_457 are separated by 73 nucleotides Pf1N1B4_457 and Pf1N1B4_458 overlap by 1 nucleotides Pf1N1B4_458 and Pf1N1B4_459 overlap by 14 nucleotides
Pf1N1B4_455 - COG1399 protein, clustered with ribosomal protein L32p, at 545,844 to 546,371
_455
Pf1N1B4_456 - FIG146278: Maf/YceF/YhdE family protein, at 546,476 to 547,054
_456
Pf1N1B4_457 - Periplasmic serine proteases (ClpP class), at 547,128 to 548,144
_457
Pf1N1B4_458 - Similar to phosphoglycolate phosphatase, clustered with ribosomal large subunit pseudouridine synthase C, at 548,144 to 548,806
_458
Pf1N1B4_459 - Ribosomal large subunit pseudouridine synthase C (EC 4.2.1.70), at 548,793 to 549,755
_459
Position (kb) 547
548
549 Strain fitness (log2 ratio) -2
-1
0
1
2 at 546.486 kb on + strand at 546.487 kb on - strand at 546.487 kb on - strand at 546.489 kb on + strand at 546.490 kb on - strand at 546.609 kb on + strand, within Pf1N1B4_456 at 546.610 kb on - strand at 546.750 kb on + strand, within Pf1N1B4_456 at 546.750 kb on + strand, within Pf1N1B4_456 at 546.750 kb on + strand, within Pf1N1B4_456 at 546.750 kb on + strand, within Pf1N1B4_456 at 546.751 kb on - strand, within Pf1N1B4_456 at 546.751 kb on - strand, within Pf1N1B4_456 at 546.751 kb on - strand, within Pf1N1B4_456 at 546.751 kb on - strand, within Pf1N1B4_456 at 546.751 kb on - strand, within Pf1N1B4_456 at 546.849 kb on + strand, within Pf1N1B4_456 at 546.850 kb on - strand, within Pf1N1B4_456 at 546.888 kb on - strand, within Pf1N1B4_456 at 546.908 kb on + strand, within Pf1N1B4_456 at 546.908 kb on + strand, within Pf1N1B4_456 at 547.020 kb on + strand at 547.022 kb on - strand at 547.022 kb on - strand at 547.022 kb on - strand at 547.048 kb on - strand at 547.404 kb on - strand, within Pf1N1B4_457 at 547.513 kb on + strand, within Pf1N1B4_457 at 547.514 kb on - strand, within Pf1N1B4_457 at 547.514 kb on - strand, within Pf1N1B4_457 at 547.514 kb on - strand, within Pf1N1B4_457 at 547.514 kb on - strand, within Pf1N1B4_457 at 547.514 kb on - strand, within Pf1N1B4_457 at 547.514 kb on - strand, within Pf1N1B4_457 at 547.518 kb on - strand, within Pf1N1B4_457 at 547.612 kb on + strand, within Pf1N1B4_457 at 547.613 kb on - strand, within Pf1N1B4_457 at 547.639 kb on + strand at 547.639 kb on + strand, within Pf1N1B4_457 at 547.639 kb on + strand, within Pf1N1B4_457 at 547.640 kb on - strand, within Pf1N1B4_457 at 547.640 kb on - strand, within Pf1N1B4_457 at 547.640 kb on - strand, within Pf1N1B4_457 at 547.640 kb on - strand, within Pf1N1B4_457 at 547.640 kb on - strand, within Pf1N1B4_457 at 547.640 kb on - strand, within Pf1N1B4_457 at 547.640 kb on - strand, within Pf1N1B4_457 at 547.640 kb on - strand, within Pf1N1B4_457 at 547.640 kb on - strand, within Pf1N1B4_457 at 547.640 kb on - strand, within Pf1N1B4_457 at 547.642 kb on + strand, within Pf1N1B4_457 at 547.642 kb on + strand at 547.643 kb on - strand, within Pf1N1B4_457 at 547.688 kb on + strand at 547.689 kb on - strand, within Pf1N1B4_457 at 547.733 kb on - strand, within Pf1N1B4_457 at 547.873 kb on - strand, within Pf1N1B4_457 at 547.873 kb on - strand, within Pf1N1B4_457 at 547.873 kb on - strand, within Pf1N1B4_457 at 547.874 kb on + strand, within Pf1N1B4_457 at 547.875 kb on - strand, within Pf1N1B4_457 at 547.927 kb on - strand, within Pf1N1B4_457 at 547.927 kb on - strand, within Pf1N1B4_457 at 547.964 kb on - strand, within Pf1N1B4_457 at 548.050 kb on - strand at 548.122 kb on + strand at 548.122 kb on + strand at 548.123 kb on - strand at 548.123 kb on - strand at 548.123 kb on - strand at 548.125 kb on - strand at 548.125 kb on - strand at 548.125 kb on - strand at 548.125 kb on - strand at 548.146 kb on - strand at 548.241 kb on + strand at 548.241 kb on + strand, within Pf1N1B4_458 at 548.416 kb on - strand, within Pf1N1B4_458 at 548.416 kb on - strand, within Pf1N1B4_458 at 548.416 kb on - strand, within Pf1N1B4_458 at 548.416 kb on - strand, within Pf1N1B4_458 at 548.568 kb on + strand, within Pf1N1B4_458 at 548.568 kb on + strand, within Pf1N1B4_458 at 548.569 kb on - strand, within Pf1N1B4_458 at 548.569 kb on - strand, within Pf1N1B4_458 at 548.569 kb on - strand, within Pf1N1B4_458 at 548.598 kb on - strand, within Pf1N1B4_458 at 548.791 kb on - strand at 548.791 kb on - strand at 548.791 kb on - strand at 548.791 kb on - strand at 548.791 kb on - strand at 548.897 kb on - strand, within Pf1N1B4_459 at 549.002 kb on + strand, within Pf1N1B4_459
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 546,486 + +1.4 546,487 - -0.6 546,487 - +0.4 546,489 + +0.7 546,490 - +0.4 546,609 + Pf1N1B4_456 0.23 +0.1 546,610 - +1.1 546,750 + Pf1N1B4_456 0.47 -0.5 546,750 + Pf1N1B4_456 0.47 -0.1 546,750 + Pf1N1B4_456 0.47 -0.3 546,750 + Pf1N1B4_456 0.47 +0.7 546,751 - Pf1N1B4_456 0.47 +1.6 546,751 - Pf1N1B4_456 0.47 -0.3 546,751 - Pf1N1B4_456 0.47 +0.2 546,751 - Pf1N1B4_456 0.47 -2.1 546,751 - Pf1N1B4_456 0.47 -1.6 546,849 + Pf1N1B4_456 0.64 +0.8 546,850 - Pf1N1B4_456 0.65 +0.7 546,888 - Pf1N1B4_456 0.71 +0.7 546,908 + Pf1N1B4_456 0.75 -1.4 546,908 + Pf1N1B4_456 0.75 -0.7 547,020 + +0.1 547,022 - +1.7 547,022 - -0.4 547,022 - -0.3 547,048 - -1.0 547,404 - Pf1N1B4_457 0.27 -0.7 547,513 + Pf1N1B4_457 0.38 -0.5 547,514 - Pf1N1B4_457 0.38 +1.1 547,514 - Pf1N1B4_457 0.38 -0.1 547,514 - Pf1N1B4_457 0.38 -1.3 547,514 - Pf1N1B4_457 0.38 -1.9 547,514 - Pf1N1B4_457 0.38 +0.1 547,514 - Pf1N1B4_457 0.38 -0.9 547,518 - Pf1N1B4_457 0.38 +1.1 547,612 + Pf1N1B4_457 0.48 -1.4 547,613 - Pf1N1B4_457 0.48 +1.1 547,639 + +0.5 547,639 + Pf1N1B4_457 0.50 -1.9 547,639 + Pf1N1B4_457 0.50 -1.3 547,640 - Pf1N1B4_457 0.50 -1.6 547,640 - Pf1N1B4_457 0.50 -0.4 547,640 - Pf1N1B4_457 0.50 -0.9 547,640 - Pf1N1B4_457 0.50 -0.5 547,640 - Pf1N1B4_457 0.50 -1.0 547,640 - Pf1N1B4_457 0.50 -2.3 547,640 - Pf1N1B4_457 0.50 +0.6 547,640 - Pf1N1B4_457 0.50 +0.7 547,640 - Pf1N1B4_457 0.50 -1.3 547,640 - Pf1N1B4_457 0.50 -1.1 547,642 + Pf1N1B4_457 0.51 -1.6 547,642 + -0.3 547,643 - Pf1N1B4_457 0.51 -0.6 547,688 + -1.3 547,689 - Pf1N1B4_457 0.55 -0.9 547,733 - Pf1N1B4_457 0.59 -0.9 547,873 - Pf1N1B4_457 0.73 -0.1 547,873 - Pf1N1B4_457 0.73 -0.4 547,873 - Pf1N1B4_457 0.73 +0.1 547,874 + Pf1N1B4_457 0.73 +0.3 547,875 - Pf1N1B4_457 0.73 -0.9 547,927 - Pf1N1B4_457 0.79 +0.3 547,927 - Pf1N1B4_457 0.79 -0.3 547,964 - Pf1N1B4_457 0.82 -0.6 548,050 - -1.9 548,122 + +0.2 548,122 + +1.1 548,123 - -0.9 548,123 - -0.9 548,123 - -2.1 548,125 - +1.5 548,125 - -0.3 548,125 - -2.4 548,125 - -0.7 548,146 - +0.7 548,241 + -0.0 548,241 + Pf1N1B4_458 0.15 -0.1 548,416 - Pf1N1B4_458 0.41 -0.2 548,416 - Pf1N1B4_458 0.41 +0.3 548,416 - Pf1N1B4_458 0.41 -0.9 548,416 - Pf1N1B4_458 0.41 +0.1 548,568 + Pf1N1B4_458 0.64 -2.4 548,568 + Pf1N1B4_458 0.64 -0.3 548,569 - Pf1N1B4_458 0.64 -0.3 548,569 - Pf1N1B4_458 0.64 +1.9 548,569 - Pf1N1B4_458 0.64 +0.7 548,598 - Pf1N1B4_458 0.68 -0.0 548,791 - -1.7 548,791 - +0.1 548,791 - +0.4 548,791 - +0.7 548,791 - +0.2 548,897 - Pf1N1B4_459 0.11 -0.9 549,002 + Pf1N1B4_459 0.22 +0.7
Or see this region's nucleotide sequence