Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4529

Experiment: Spectinomycin 0.025 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4528 and Pf1N1B4_4529 are separated by 190 nucleotidesPf1N1B4_4529 and Pf1N1B4_4530 are separated by 336 nucleotidesPf1N1B4_4530 and Pf1N1B4_4531 are separated by 12 nucleotides Pf1N1B4_4528 - Predicted aminoglycoside phosphotransferase, at 4,991,950 to 4,993,017 _4528 Pf1N1B4_4529 - FIG00966904: hypothetical protein, at 4,993,208 to 4,993,603 _4529 Pf1N1B4_4530 - Phophatidylinositol-4-phosphate 5-kinase (EC 2.7.1.68), at 4,993,940 to 4,994,482 _4530 Pf1N1B4_4531 - FIG01221261: hypothetical protein, at 4,994,495 to 4,996,504 _4531 Position (kb) 4993 4994Strain fitness (log2 ratio) -1 0 1at 4992.447 kb on + strandat 4992.512 kb on + strand, within Pf1N1B4_4528at 4992.555 kb on - strand, within Pf1N1B4_4528at 4992.686 kb on + strand, within Pf1N1B4_4528at 4992.728 kb on + strand, within Pf1N1B4_4528at 4992.728 kb on + strand, within Pf1N1B4_4528at 4992.729 kb on - strand, within Pf1N1B4_4528at 4992.729 kb on - strand, within Pf1N1B4_4528at 4993.008 kb on + strandat 4993.030 kb on + strandat 4993.065 kb on + strandat 4993.106 kb on + strandat 4993.106 kb on + strandat 4993.106 kb on + strandat 4993.106 kb on + strandat 4993.106 kb on + strandat 4993.107 kb on - strandat 4993.107 kb on - strandat 4993.107 kb on - strandat 4993.167 kb on - strandat 4993.273 kb on - strand, within Pf1N1B4_4529at 4993.739 kb on + strandat 4993.868 kb on - strandat 4993.868 kb on - strandat 4994.143 kb on - strand, within Pf1N1B4_4530at 4994.143 kb on - strand, within Pf1N1B4_4530at 4994.179 kb on - strand, within Pf1N1B4_4530at 4994.322 kb on + strand, within Pf1N1B4_4530

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Per-strain Table

Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml
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4,992,447 + +0.3
4,992,512 + Pf1N1B4_4528 0.53 +0.8
4,992,555 - Pf1N1B4_4528 0.57 +1.1
4,992,686 + Pf1N1B4_4528 0.69 -0.1
4,992,728 + Pf1N1B4_4528 0.73 -0.7
4,992,728 + Pf1N1B4_4528 0.73 -1.0
4,992,729 - Pf1N1B4_4528 0.73 +0.3
4,992,729 - Pf1N1B4_4528 0.73 +0.0
4,993,008 + -1.5
4,993,030 + +0.1
4,993,065 + -0.7
4,993,106 + -0.7
4,993,106 + -0.9
4,993,106 + +0.6
4,993,106 + -0.9
4,993,106 + +0.7
4,993,107 - +1.3
4,993,107 - -1.2
4,993,107 - +0.9
4,993,167 - -1.5
4,993,273 - Pf1N1B4_4529 0.16 +0.9
4,993,739 + +0.1
4,993,868 - +1.0
4,993,868 - +0.1
4,994,143 - Pf1N1B4_4530 0.37 -0.2
4,994,143 - Pf1N1B4_4530 0.37 +0.7
4,994,179 - Pf1N1B4_4530 0.44 -0.8
4,994,322 + Pf1N1B4_4530 0.70 +1.4

Or see this region's nucleotide sequence