Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4526 and Pf1N1B4_4527 are separated by 459 nucleotides Pf1N1B4_4527 and Pf1N1B4_4528 are separated by 24 nucleotides
Pf1N1B4_4526 - Myo-inositol 2-dehydrogenase (EC 1.1.1.18), at 4,989,583 to 4,990,698
_4526
Pf1N1B4_4527 - Short-chain dehydrogenase/reductase SDR, at 4,991,158 to 4,991,925
_4527
Pf1N1B4_4528 - Predicted aminoglycoside phosphotransferase, at 4,991,950 to 4,993,017
_4528
Position (kb)
4991
4992 Strain fitness (log2 ratio)
-2
-1
0
1 at 4990.304 kb on + strand, within Pf1N1B4_4526 at 4990.304 kb on + strand, within Pf1N1B4_4526 at 4990.308 kb on + strand, within Pf1N1B4_4526 at 4990.308 kb on + strand, within Pf1N1B4_4526 at 4990.309 kb on - strand, within Pf1N1B4_4526 at 4990.401 kb on - strand, within Pf1N1B4_4526 at 4990.943 kb on + strand at 4990.963 kb on + strand at 4990.964 kb on - strand at 4990.978 kb on - strand at 4990.978 kb on - strand at 4991.105 kb on - strand at 4991.105 kb on - strand at 4991.204 kb on + strand at 4991.205 kb on - strand at 4991.228 kb on + strand at 4991.228 kb on + strand at 4991.228 kb on + strand at 4991.228 kb on + strand at 4991.229 kb on - strand at 4991.318 kb on - strand, within Pf1N1B4_4527 at 4991.444 kb on + strand, within Pf1N1B4_4527 at 4991.444 kb on + strand, within Pf1N1B4_4527 at 4991.444 kb on + strand, within Pf1N1B4_4527 at 4991.445 kb on - strand at 4991.445 kb on - strand at 4991.445 kb on - strand at 4991.445 kb on - strand, within Pf1N1B4_4527 at 4991.472 kb on + strand, within Pf1N1B4_4527 at 4991.472 kb on + strand at 4991.473 kb on - strand, within Pf1N1B4_4527 at 4992.062 kb on + strand, within Pf1N1B4_4528 at 4992.062 kb on + strand, within Pf1N1B4_4528 at 4992.063 kb on - strand, within Pf1N1B4_4528 at 4992.063 kb on - strand, within Pf1N1B4_4528 at 4992.101 kb on + strand at 4992.107 kb on + strand, within Pf1N1B4_4528 at 4992.108 kb on - strand, within Pf1N1B4_4528 at 4992.110 kb on + strand, within Pf1N1B4_4528 at 4992.111 kb on - strand at 4992.152 kb on + strand, within Pf1N1B4_4528 at 4992.447 kb on + strand at 4992.512 kb on + strand, within Pf1N1B4_4528 at 4992.555 kb on - strand, within Pf1N1B4_4528 at 4992.686 kb on + strand, within Pf1N1B4_4528 at 4992.728 kb on + strand, within Pf1N1B4_4528 at 4992.728 kb on + strand, within Pf1N1B4_4528 at 4992.729 kb on - strand, within Pf1N1B4_4528 at 4992.729 kb on - strand, within Pf1N1B4_4528
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 4,990,304 + Pf1N1B4_4526 0.65 +0.7 4,990,304 + Pf1N1B4_4526 0.65 +0.7 4,990,308 + Pf1N1B4_4526 0.65 +0.1 4,990,308 + Pf1N1B4_4526 0.65 +0.8 4,990,309 - Pf1N1B4_4526 0.65 +0.3 4,990,401 - Pf1N1B4_4526 0.73 -1.4 4,990,943 + -0.7 4,990,963 + -0.9 4,990,964 - -0.1 4,990,978 - +0.6 4,990,978 - +0.5 4,991,105 - -0.7 4,991,105 - +1.1 4,991,204 + -0.2 4,991,205 - -0.3 4,991,228 + +1.7 4,991,228 + -0.8 4,991,228 + -0.5 4,991,228 + -0.9 4,991,229 - +0.5 4,991,318 - Pf1N1B4_4527 0.21 +0.6 4,991,444 + Pf1N1B4_4527 0.37 -0.5 4,991,444 + Pf1N1B4_4527 0.37 +0.6 4,991,444 + Pf1N1B4_4527 0.37 +1.7 4,991,445 - +1.7 4,991,445 - +1.3 4,991,445 - +1.3 4,991,445 - Pf1N1B4_4527 0.37 -0.8 4,991,472 + Pf1N1B4_4527 0.41 -0.7 4,991,472 + +0.9 4,991,473 - Pf1N1B4_4527 0.41 -2.3 4,992,062 + Pf1N1B4_4528 0.10 -0.2 4,992,062 + Pf1N1B4_4528 0.10 +0.9 4,992,063 - Pf1N1B4_4528 0.11 -0.2 4,992,063 - Pf1N1B4_4528 0.11 +1.0 4,992,101 + +0.7 4,992,107 + Pf1N1B4_4528 0.15 -1.7 4,992,108 - Pf1N1B4_4528 0.15 -1.5 4,992,110 + Pf1N1B4_4528 0.15 +1.1 4,992,111 - -0.2 4,992,152 + Pf1N1B4_4528 0.19 -0.6 4,992,447 + +0.3 4,992,512 + Pf1N1B4_4528 0.53 +0.8 4,992,555 - Pf1N1B4_4528 0.57 +1.1 4,992,686 + Pf1N1B4_4528 0.69 -0.1 4,992,728 + Pf1N1B4_4528 0.73 -0.7 4,992,728 + Pf1N1B4_4528 0.73 -1.0 4,992,729 - Pf1N1B4_4528 0.73 +0.3 4,992,729 - Pf1N1B4_4528 0.73 +0.0
Or see this region's nucleotide sequence