Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4499 and Pf1N1B4_4500 are separated by 147 nucleotides Pf1N1B4_4500 and Pf1N1B4_4501 overlap by 24 nucleotides Pf1N1B4_4501 and Pf1N1B4_4502 are separated by 240 nucleotides
Pf1N1B4_4499 - SoxH protein, homolog, at 4,960,425 to 4,961,333
_4499
Pf1N1B4_4500 - hypothetical protein, at 4,961,481 to 4,961,648
_4500
Pf1N1B4_4501 - Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.99.8), at 4,961,625 to 4,963,400
_4501
Pf1N1B4_4502 - Aldehyde dehydrogenase (EC 1.2.1.3), at 4,963,641 to 4,965,161
_4502
Position (kb) 4961
4962
4963
4964 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4960.828 kb on - strand, within Pf1N1B4_4499 at 4961.509 kb on - strand at 4961.516 kb on + strand, within Pf1N1B4_4500 at 4961.598 kb on - strand, within Pf1N1B4_4500 at 4961.916 kb on + strand, within Pf1N1B4_4501 at 4961.916 kb on + strand, within Pf1N1B4_4501 at 4961.917 kb on - strand, within Pf1N1B4_4501 at 4961.917 kb on - strand, within Pf1N1B4_4501 at 4961.917 kb on - strand, within Pf1N1B4_4501 at 4962.156 kb on + strand, within Pf1N1B4_4501 at 4962.315 kb on + strand, within Pf1N1B4_4501 at 4962.315 kb on + strand, within Pf1N1B4_4501 at 4962.315 kb on + strand, within Pf1N1B4_4501 at 4962.315 kb on + strand, within Pf1N1B4_4501 at 4962.316 kb on - strand, within Pf1N1B4_4501 at 4962.510 kb on + strand at 4962.510 kb on + strand, within Pf1N1B4_4501 at 4962.627 kb on + strand, within Pf1N1B4_4501 at 4962.627 kb on + strand at 4962.628 kb on - strand, within Pf1N1B4_4501 at 4962.628 kb on - strand, within Pf1N1B4_4501 at 4962.687 kb on + strand, within Pf1N1B4_4501 at 4962.787 kb on - strand at 4962.787 kb on - strand, within Pf1N1B4_4501 at 4962.862 kb on + strand, within Pf1N1B4_4501 at 4962.915 kb on + strand at 4962.915 kb on + strand, within Pf1N1B4_4501 at 4962.915 kb on + strand, within Pf1N1B4_4501 at 4962.915 kb on + strand, within Pf1N1B4_4501 at 4962.915 kb on + strand, within Pf1N1B4_4501 at 4962.915 kb on + strand, within Pf1N1B4_4501 at 4962.916 kb on - strand at 4962.916 kb on - strand, within Pf1N1B4_4501 at 4962.919 kb on + strand, within Pf1N1B4_4501 at 4962.919 kb on + strand, within Pf1N1B4_4501 at 4962.966 kb on + strand, within Pf1N1B4_4501 at 4963.026 kb on + strand, within Pf1N1B4_4501 at 4963.027 kb on - strand, within Pf1N1B4_4501 at 4963.263 kb on + strand at 4963.264 kb on - strand at 4963.523 kb on + strand at 4963.523 kb on + strand at 4963.524 kb on - strand at 4963.584 kb on + strand at 4963.584 kb on + strand at 4963.584 kb on + strand at 4963.585 kb on - strand at 4963.647 kb on + strand at 4963.648 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 4,960,828 - Pf1N1B4_4499 0.44 -1.9 4,961,509 - +1.9 4,961,516 + Pf1N1B4_4500 0.21 +0.1 4,961,598 - Pf1N1B4_4500 0.70 -0.6 4,961,916 + Pf1N1B4_4501 0.16 +0.4 4,961,916 + Pf1N1B4_4501 0.16 -1.6 4,961,917 - Pf1N1B4_4501 0.16 -0.1 4,961,917 - Pf1N1B4_4501 0.16 +0.9 4,961,917 - Pf1N1B4_4501 0.16 +0.4 4,962,156 + Pf1N1B4_4501 0.30 +0.7 4,962,315 + Pf1N1B4_4501 0.39 -1.4 4,962,315 + Pf1N1B4_4501 0.39 +0.8 4,962,315 + Pf1N1B4_4501 0.39 -0.9 4,962,315 + Pf1N1B4_4501 0.39 -0.3 4,962,316 - Pf1N1B4_4501 0.39 +1.2 4,962,510 + -1.2 4,962,510 + Pf1N1B4_4501 0.50 +1.7 4,962,627 + Pf1N1B4_4501 0.56 +0.4 4,962,627 + -1.2 4,962,628 - Pf1N1B4_4501 0.56 +2.2 4,962,628 - Pf1N1B4_4501 0.56 +0.4 4,962,687 + Pf1N1B4_4501 0.60 -0.5 4,962,787 - +2.4 4,962,787 - Pf1N1B4_4501 0.65 +0.4 4,962,862 + Pf1N1B4_4501 0.70 +1.4 4,962,915 + +0.4 4,962,915 + Pf1N1B4_4501 0.73 +1.2 4,962,915 + Pf1N1B4_4501 0.73 +1.4 4,962,915 + Pf1N1B4_4501 0.73 +0.4 4,962,915 + Pf1N1B4_4501 0.73 -0.1 4,962,915 + Pf1N1B4_4501 0.73 -0.6 4,962,916 - +0.9 4,962,916 - Pf1N1B4_4501 0.73 +0.6 4,962,919 + Pf1N1B4_4501 0.73 -1.0 4,962,919 + Pf1N1B4_4501 0.73 +0.4 4,962,966 + Pf1N1B4_4501 0.76 -1.5 4,963,026 + Pf1N1B4_4501 0.79 +0.4 4,963,027 - Pf1N1B4_4501 0.79 +1.1 4,963,263 + +1.2 4,963,264 - +1.1 4,963,523 + -1.4 4,963,523 + +0.8 4,963,524 - +0.2 4,963,584 + +0.4 4,963,584 + +0.6 4,963,584 + -0.4 4,963,585 - -0.6 4,963,647 + +0.9 4,963,648 - +0.7
Or see this region's nucleotide sequence