Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4474 and Pf1N1B4_4475 overlap by 11 nucleotides Pf1N1B4_4475 and Pf1N1B4_4476 are separated by 135 nucleotides Pf1N1B4_4476 and Pf1N1B4_4477 are separated by 234 nucleotides
Pf1N1B4_4474 - transcription regulator protein, at 4,935,131 to 4,936,057
_4474
Pf1N1B4_4475 - hypothetical protein, at 4,936,047 to 4,936,388
_4475
Pf1N1B4_4476 - Branched-chain amino acid aminotransferase (EC 2.6.1.42), at 4,936,524 to 4,937,543
_4476
Pf1N1B4_4477 - Dihydrolipoamide dehydrogenase of branched-chain alpha-keto acid dehydrogenase (EC 1.8.1.4), at 4,937,778 to 4,939,160
_4477
Position (kb)
4936
4937
4938 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4935.627 kb on + strand, within Pf1N1B4_4474 at 4935.628 kb on - strand at 4935.628 kb on - strand, within Pf1N1B4_4474 at 4935.935 kb on + strand, within Pf1N1B4_4474 at 4936.099 kb on + strand, within Pf1N1B4_4475 at 4936.175 kb on + strand, within Pf1N1B4_4475 at 4936.176 kb on - strand, within Pf1N1B4_4475 at 4936.367 kb on - strand at 4936.432 kb on + strand at 4936.573 kb on - strand at 4936.591 kb on + strand at 4936.654 kb on + strand, within Pf1N1B4_4476 at 4936.655 kb on - strand, within Pf1N1B4_4476 at 4936.655 kb on - strand, within Pf1N1B4_4476 at 4936.692 kb on - strand, within Pf1N1B4_4476 at 4936.918 kb on + strand, within Pf1N1B4_4476 at 4936.919 kb on - strand, within Pf1N1B4_4476 at 4936.978 kb on + strand, within Pf1N1B4_4476 at 4936.978 kb on + strand, within Pf1N1B4_4476 at 4936.979 kb on - strand, within Pf1N1B4_4476 at 4936.979 kb on - strand, within Pf1N1B4_4476 at 4936.979 kb on - strand, within Pf1N1B4_4476 at 4937.387 kb on - strand, within Pf1N1B4_4476 at 4937.500 kb on + strand at 4937.501 kb on - strand at 4937.554 kb on - strand at 4937.616 kb on + strand at 4937.616 kb on + strand at 4937.616 kb on + strand at 4937.616 kb on + strand at 4937.616 kb on + strand at 4937.616 kb on + strand at 4937.617 kb on - strand at 4937.617 kb on - strand at 4937.617 kb on - strand at 4937.617 kb on - strand at 4937.617 kb on - strand at 4937.617 kb on - strand at 4937.617 kb on - strand at 4937.780 kb on - strand at 4938.254 kb on - strand, within Pf1N1B4_4477 at 4938.431 kb on + strand, within Pf1N1B4_4477 at 4938.431 kb on + strand, within Pf1N1B4_4477 at 4938.432 kb on - strand, within Pf1N1B4_4477 at 4938.533 kb on - strand, within Pf1N1B4_4477 at 4938.533 kb on - strand, within Pf1N1B4_4477
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 4,935,627 + Pf1N1B4_4474 0.54 -0.6 4,935,628 - -1.2 4,935,628 - Pf1N1B4_4474 0.54 +0.4 4,935,935 + Pf1N1B4_4474 0.87 +1.9 4,936,099 + Pf1N1B4_4475 0.15 -0.5 4,936,175 + Pf1N1B4_4475 0.37 +1.2 4,936,176 - Pf1N1B4_4475 0.38 -2.2 4,936,367 - +0.4 4,936,432 + -0.2 4,936,573 - +0.5 4,936,591 + -0.2 4,936,654 + Pf1N1B4_4476 0.13 -1.5 4,936,655 - Pf1N1B4_4476 0.13 -1.3 4,936,655 - Pf1N1B4_4476 0.13 -0.5 4,936,692 - Pf1N1B4_4476 0.16 +0.4 4,936,918 + Pf1N1B4_4476 0.39 -1.1 4,936,919 - Pf1N1B4_4476 0.39 -2.0 4,936,978 + Pf1N1B4_4476 0.45 -0.9 4,936,978 + Pf1N1B4_4476 0.45 -0.0 4,936,979 - Pf1N1B4_4476 0.45 -0.1 4,936,979 - Pf1N1B4_4476 0.45 +0.0 4,936,979 - Pf1N1B4_4476 0.45 -1.2 4,937,387 - Pf1N1B4_4476 0.85 -0.1 4,937,500 + +1.1 4,937,501 - +1.6 4,937,554 - +0.5 4,937,616 + +0.4 4,937,616 + -1.1 4,937,616 + +0.4 4,937,616 + -1.2 4,937,616 + -0.1 4,937,616 + -0.4 4,937,617 - +2.2 4,937,617 - +0.4 4,937,617 - +1.7 4,937,617 - +0.4 4,937,617 - +0.4 4,937,617 - +0.4 4,937,617 - -0.3 4,937,780 - +1.0 4,938,254 - Pf1N1B4_4477 0.34 -1.1 4,938,431 + Pf1N1B4_4477 0.47 +0.2 4,938,431 + Pf1N1B4_4477 0.47 +1.1 4,938,432 - Pf1N1B4_4477 0.47 +2.4 4,938,533 - Pf1N1B4_4477 0.55 +1.1 4,938,533 - Pf1N1B4_4477 0.55 -0.1
Or see this region's nucleotide sequence