Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4403 and Pf1N1B4_4404 are separated by 853 nucleotides Pf1N1B4_4404 and Pf1N1B4_4405 are separated by 227 nucleotides
Pf1N1B4_4403 - NADH dehydrogenase (EC 1.6.99.3), at 4,862,589 to 4,863,839
_4403
Pf1N1B4_4404 - Transcriptional regulator, LysR family, at 4,864,693 to 4,865,658
_4404
Pf1N1B4_4405 - Signal transduction histidine kinase CheA (EC 2.7.3.-), at 4,865,886 to 4,867,397
_4405
Position (kb)
4864
4865
4866 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4863.780 kb on - strand at 4863.855 kb on - strand at 4863.858 kb on + strand at 4863.859 kb on - strand at 4863.859 kb on - strand at 4863.969 kb on + strand at 4863.970 kb on - strand at 4863.988 kb on + strand at 4863.988 kb on + strand at 4863.991 kb on - strand at 4864.125 kb on - strand at 4864.168 kb on + strand at 4864.168 kb on + strand at 4864.169 kb on - strand at 4864.170 kb on + strand at 4864.171 kb on - strand at 4864.172 kb on + strand at 4864.309 kb on + strand at 4864.491 kb on + strand at 4864.601 kb on + strand at 4864.601 kb on + strand at 4864.624 kb on + strand at 4864.625 kb on - strand at 4864.625 kb on - strand at 4864.639 kb on + strand at 4864.639 kb on + strand at 4864.643 kb on + strand at 4864.644 kb on - strand at 4865.056 kb on + strand, within Pf1N1B4_4404 at 4865.249 kb on + strand, within Pf1N1B4_4404 at 4865.249 kb on + strand, within Pf1N1B4_4404 at 4865.249 kb on + strand, within Pf1N1B4_4404 at 4865.442 kb on + strand, within Pf1N1B4_4404 at 4865.464 kb on + strand, within Pf1N1B4_4404 at 4865.465 kb on - strand at 4865.667 kb on + strand at 4865.754 kb on + strand at 4865.754 kb on + strand at 4865.775 kb on + strand at 4865.775 kb on + strand at 4865.775 kb on + strand at 4865.775 kb on + strand at 4865.775 kb on + strand at 4865.775 kb on + strand at 4865.854 kb on + strand at 4865.855 kb on - strand at 4865.855 kb on - strand at 4865.855 kb on - strand at 4865.858 kb on + strand at 4865.858 kb on + strand at 4865.858 kb on + strand at 4865.859 kb on - strand at 4865.859 kb on - strand at 4865.859 kb on - strand at 4865.859 kb on - strand at 4865.859 kb on - strand at 4865.859 kb on - strand at 4865.893 kb on + strand at 4865.893 kb on + strand at 4865.894 kb on - strand at 4865.896 kb on + strand at 4865.916 kb on - strand at 4866.102 kb on + strand, within Pf1N1B4_4405 at 4866.103 kb on - strand, within Pf1N1B4_4405 at 4866.103 kb on - strand at 4866.103 kb on - strand, within Pf1N1B4_4405 at 4866.245 kb on + strand, within Pf1N1B4_4405 at 4866.245 kb on + strand, within Pf1N1B4_4405 at 4866.296 kb on + strand, within Pf1N1B4_4405 at 4866.369 kb on + strand, within Pf1N1B4_4405 at 4866.369 kb on + strand, within Pf1N1B4_4405 at 4866.370 kb on - strand at 4866.371 kb on + strand, within Pf1N1B4_4405
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 4,863,780 - +0.2 4,863,855 - +0.4 4,863,858 + -1.2 4,863,859 - +0.2 4,863,859 - -1.2 4,863,969 + -0.8 4,863,970 - -0.6 4,863,988 + +0.8 4,863,988 + -0.8 4,863,991 - +1.8 4,864,125 - +0.5 4,864,168 + -1.1 4,864,168 + +0.0 4,864,169 - -0.1 4,864,170 + -1.2 4,864,171 - +0.3 4,864,172 + +1.2 4,864,309 + -1.2 4,864,491 + -0.9 4,864,601 + -0.2 4,864,601 + +0.4 4,864,624 + -0.6 4,864,625 - +1.1 4,864,625 - -0.6 4,864,639 + +0.6 4,864,639 + +0.8 4,864,643 + +0.3 4,864,644 - -1.0 4,865,056 + Pf1N1B4_4404 0.38 +0.4 4,865,249 + Pf1N1B4_4404 0.58 -0.6 4,865,249 + Pf1N1B4_4404 0.58 +0.1 4,865,249 + Pf1N1B4_4404 0.58 -0.5 4,865,442 + Pf1N1B4_4404 0.78 -0.7 4,865,464 + Pf1N1B4_4404 0.80 -1.2 4,865,465 - +0.9 4,865,667 + -1.0 4,865,754 + -0.5 4,865,754 + -0.2 4,865,775 + +2.7 4,865,775 + +0.4 4,865,775 + -1.4 4,865,775 + -0.4 4,865,775 + -1.2 4,865,775 + -0.1 4,865,854 + +1.4 4,865,855 - +0.4 4,865,855 - -0.3 4,865,855 - +0.7 4,865,858 + -2.2 4,865,858 + -0.6 4,865,858 + -0.0 4,865,859 - -2.4 4,865,859 - -0.8 4,865,859 - -0.2 4,865,859 - -0.2 4,865,859 - -0.6 4,865,859 - -0.6 4,865,893 + +0.6 4,865,893 + +1.7 4,865,894 - +0.4 4,865,896 + +1.5 4,865,916 - -0.4 4,866,102 + Pf1N1B4_4405 0.14 -0.6 4,866,103 - Pf1N1B4_4405 0.14 -0.6 4,866,103 - -0.6 4,866,103 - Pf1N1B4_4405 0.14 +1.1 4,866,245 + Pf1N1B4_4405 0.24 -0.6 4,866,245 + Pf1N1B4_4405 0.24 -0.2 4,866,296 + Pf1N1B4_4405 0.27 -1.0 4,866,369 + Pf1N1B4_4405 0.32 -0.1 4,866,369 + Pf1N1B4_4405 0.32 +1.0 4,866,370 - +0.9 4,866,371 + Pf1N1B4_4405 0.32 -1.0
Or see this region's nucleotide sequence