Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4331 and Pf1N1B4_4332 are separated by 188 nucleotides Pf1N1B4_4332 and Pf1N1B4_4333 are separated by 50 nucleotides Pf1N1B4_4333 and Pf1N1B4_4334 are separated by 356 nucleotides
Pf1N1B4_4331 - Transporter, LysE family, at 4,791,582 to 4,792,199
_4331
Pf1N1B4_4332 - Transcriptional regulator, AsnC family, at 4,792,388 to 4,792,840
_4332
Pf1N1B4_4333 - hypothetical protein, at 4,792,891 to 4,793,112
_4333
Pf1N1B4_4334 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 4,793,469 to 4,794,452
_4334
Position (kb)
4792
4793
4794 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4792.053 kb on + strand at 4792.147 kb on - strand at 4792.148 kb on + strand at 4792.149 kb on - strand at 4792.149 kb on - strand at 4792.149 kb on - strand at 4792.149 kb on - strand at 4792.199 kb on + strand at 4792.200 kb on - strand at 4792.214 kb on + strand at 4792.214 kb on + strand at 4792.215 kb on - strand at 4792.215 kb on - strand at 4792.240 kb on + strand at 4792.300 kb on - strand at 4792.318 kb on - strand at 4792.348 kb on + strand at 4792.474 kb on + strand, within Pf1N1B4_4332 at 4792.697 kb on + strand, within Pf1N1B4_4332 at 4792.698 kb on - strand, within Pf1N1B4_4332 at 4792.893 kb on - strand at 4792.918 kb on + strand, within Pf1N1B4_4333 at 4792.919 kb on - strand, within Pf1N1B4_4333 at 4792.919 kb on - strand, within Pf1N1B4_4333 at 4792.979 kb on - strand, within Pf1N1B4_4333 at 4792.979 kb on - strand, within Pf1N1B4_4333 at 4793.046 kb on + strand, within Pf1N1B4_4333 at 4793.047 kb on - strand at 4793.134 kb on - strand at 4793.376 kb on - strand at 4793.388 kb on + strand at 4793.420 kb on + strand at 4793.421 kb on - strand at 4793.961 kb on + strand, within Pf1N1B4_4334 at 4793.961 kb on + strand, within Pf1N1B4_4334 at 4793.961 kb on + strand, within Pf1N1B4_4334 at 4793.962 kb on - strand, within Pf1N1B4_4334 at 4793.962 kb on - strand, within Pf1N1B4_4334
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 4,792,053 + -0.6 4,792,147 - +0.4 4,792,148 + +0.8 4,792,149 - -0.3 4,792,149 - +0.6 4,792,149 - +0.4 4,792,149 - -0.8 4,792,199 + +0.0 4,792,200 - -0.0 4,792,214 + -2.4 4,792,214 + +1.6 4,792,215 - -0.5 4,792,215 - +0.6 4,792,240 + -0.8 4,792,300 - -0.1 4,792,318 - +0.1 4,792,348 + +0.1 4,792,474 + Pf1N1B4_4332 0.19 -1.0 4,792,697 + Pf1N1B4_4332 0.68 +1.6 4,792,698 - Pf1N1B4_4332 0.68 -0.3 4,792,893 - +0.4 4,792,918 + Pf1N1B4_4333 0.12 -2.8 4,792,919 - Pf1N1B4_4333 0.13 -0.9 4,792,919 - Pf1N1B4_4333 0.13 -1.1 4,792,979 - Pf1N1B4_4333 0.40 +0.4 4,792,979 - Pf1N1B4_4333 0.40 +0.8 4,793,046 + Pf1N1B4_4333 0.70 -0.2 4,793,047 - +0.4 4,793,134 - -2.6 4,793,376 - -1.2 4,793,388 + +0.6 4,793,420 + -0.8 4,793,421 - +0.7 4,793,961 + Pf1N1B4_4334 0.50 -1.2 4,793,961 + Pf1N1B4_4334 0.50 -1.6 4,793,961 + Pf1N1B4_4334 0.50 +0.5 4,793,962 - Pf1N1B4_4334 0.50 -2.2 4,793,962 - Pf1N1B4_4334 0.50 -0.1
Or see this region's nucleotide sequence