Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_429 and Pf1N1B4_430 are separated by 666 nucleotides
Pf1N1B4_429 - FIG00964214: hypothetical protein, at 499,404 to 499,703
_429
Pf1N1B4_430 - hypothetical protein, at 500,370 to 500,486
_430
Position (kb)
500
501 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 499.627 kb on + strand, within Pf1N1B4_429 at 499.628 kb on - strand, within Pf1N1B4_429 at 499.655 kb on + strand, within Pf1N1B4_429 at 499.655 kb on + strand, within Pf1N1B4_429 at 499.655 kb on + strand, within Pf1N1B4_429 at 499.656 kb on - strand, within Pf1N1B4_429 at 499.737 kb on - strand at 499.750 kb on + strand at 499.750 kb on + strand at 499.763 kb on - strand at 499.788 kb on + strand at 499.789 kb on - strand at 499.789 kb on - strand at 499.863 kb on + strand at 499.903 kb on + strand at 499.903 kb on + strand at 499.904 kb on - strand at 499.904 kb on - strand at 499.962 kb on + strand at 499.962 kb on + strand at 499.962 kb on + strand at 499.962 kb on + strand at 499.963 kb on - strand at 499.972 kb on + strand at 500.094 kb on + strand at 500.094 kb on + strand at 500.094 kb on + strand at 500.095 kb on - strand at 500.095 kb on - strand at 500.104 kb on - strand at 500.111 kb on + strand at 500.129 kb on - strand at 500.235 kb on - strand at 500.338 kb on + strand at 500.386 kb on + strand, within Pf1N1B4_430 at 500.387 kb on - strand, within Pf1N1B4_430 at 500.387 kb on - strand, within Pf1N1B4_430 at 500.416 kb on - strand, within Pf1N1B4_430 at 500.416 kb on - strand, within Pf1N1B4_430 at 500.515 kb on + strand at 500.560 kb on + strand at 500.613 kb on + strand at 500.615 kb on + strand at 500.615 kb on + strand at 500.615 kb on + strand at 500.615 kb on + strand at 500.615 kb on + strand at 500.615 kb on + strand at 500.615 kb on + strand at 500.616 kb on - strand at 500.616 kb on - strand at 500.616 kb on - strand at 500.831 kb on + strand at 500.850 kb on - strand at 500.903 kb on - strand at 500.909 kb on - strand at 500.909 kb on - strand at 500.909 kb on - strand at 500.909 kb on - strand at 500.913 kb on - strand at 500.913 kb on - strand at 500.990 kb on - strand at 501.035 kb on - strand at 501.035 kb on - strand at 501.035 kb on - strand at 501.043 kb on + strand at 501.043 kb on + strand at 501.043 kb on + strand at 501.044 kb on - strand at 501.044 kb on - strand at 501.047 kb on + strand at 501.047 kb on + strand at 501.047 kb on + strand at 501.047 kb on + strand at 501.047 kb on + strand at 501.047 kb on + strand at 501.047 kb on + strand at 501.048 kb on - strand at 501.048 kb on - strand at 501.059 kb on + strand at 501.060 kb on - strand at 501.094 kb on + strand at 501.151 kb on + strand at 501.152 kb on - strand at 501.190 kb on - strand at 501.190 kb on - strand at 501.197 kb on - strand at 501.197 kb on - strand at 501.246 kb on + strand at 501.251 kb on + strand at 501.251 kb on + strand at 501.251 kb on + strand at 501.251 kb on + strand at 501.251 kb on + strand at 501.251 kb on + strand at 501.251 kb on + strand at 501.252 kb on - strand at 501.252 kb on - strand at 501.252 kb on - strand at 501.252 kb on - strand at 501.252 kb on - strand at 501.252 kb on - strand at 501.252 kb on - strand at 501.463 kb on - strand at 501.463 kb on - strand at 501.463 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 499,627 + Pf1N1B4_429 0.74 -1.3 499,628 - Pf1N1B4_429 0.75 +0.7 499,655 + Pf1N1B4_429 0.84 +0.7 499,655 + Pf1N1B4_429 0.84 +0.4 499,655 + Pf1N1B4_429 0.84 -0.3 499,656 - Pf1N1B4_429 0.84 +1.7 499,737 - -0.9 499,750 + +0.3 499,750 + -1.3 499,763 - -0.3 499,788 + -0.6 499,789 - +0.5 499,789 - +1.1 499,863 + +0.2 499,903 + -1.3 499,903 + -0.0 499,904 - -0.1 499,904 - +0.5 499,962 + -1.3 499,962 + +1.1 499,962 + -1.6 499,962 + +0.7 499,963 - -0.1 499,972 + -1.3 500,094 + -0.3 500,094 + -0.1 500,094 + -1.9 500,095 - +1.4 500,095 - +1.4 500,104 - -0.9 500,111 + +0.5 500,129 - +1.7 500,235 - +0.5 500,338 + +0.7 500,386 + Pf1N1B4_430 0.14 -1.5 500,387 - Pf1N1B4_430 0.15 -1.3 500,387 - Pf1N1B4_430 0.15 +0.2 500,416 - Pf1N1B4_430 0.39 +0.7 500,416 - Pf1N1B4_430 0.39 -2.1 500,515 + +1.2 500,560 + +0.3 500,613 + +0.1 500,615 + +0.5 500,615 + +1.3 500,615 + -0.7 500,615 + +0.7 500,615 + -0.3 500,615 + +1.7 500,615 + -1.4 500,616 - +0.7 500,616 - +0.2 500,616 - +2.3 500,831 + -2.0 500,850 - +0.4 500,903 - -0.6 500,909 - -0.6 500,909 - +0.7 500,909 - -0.3 500,909 - +1.5 500,913 - -1.6 500,913 - +0.0 500,990 - -0.7 501,035 - +0.2 501,035 - -0.7 501,035 - -1.0 501,043 + -0.9 501,043 + -0.5 501,043 + +1.7 501,044 - +1.3 501,044 - -0.6 501,047 + -0.1 501,047 + -0.5 501,047 + +1.0 501,047 + +0.5 501,047 + -0.6 501,047 + -1.3 501,047 + -0.3 501,048 - +0.7 501,048 - +0.7 501,059 + +0.5 501,060 - +3.0 501,094 + -0.1 501,151 + +1.0 501,152 - +0.5 501,190 - +0.1 501,190 - -0.6 501,197 - +0.7 501,197 - +0.0 501,246 + -0.2 501,251 + -0.4 501,251 + +0.9 501,251 + +0.7 501,251 + -0.4 501,251 + -1.3 501,251 + +0.7 501,251 + -1.1 501,252 - +0.5 501,252 - +0.7 501,252 - +1.2 501,252 - +2.9 501,252 - -0.9 501,252 - -0.6 501,252 - -0.9 501,463 - +0.7 501,463 - -0.3 501,463 - +1.5
Or see this region's nucleotide sequence