Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4245 and Pf1N1B4_4246 are separated by 227 nucleotides Pf1N1B4_4246 and Pf1N1B4_4247 are separated by 527 nucleotides
Pf1N1B4_4245 - glutamine synthetase family protein, at 4,704,936 to 4,706,309
_4245
Pf1N1B4_4246 - L-lactate permease, at 4,706,537 to 4,708,294
_4246
Pf1N1B4_4247 - D-aminopeptidase (EC 3.4.11.19), at 4,708,822 to 4,709,937
_4247
Position (kb)
4706
4707
4708
4709 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4705.786 kb on + strand, within Pf1N1B4_4245 at 4705.787 kb on - strand, within Pf1N1B4_4245 at 4705.798 kb on + strand, within Pf1N1B4_4245 at 4705.798 kb on + strand, within Pf1N1B4_4245 at 4705.826 kb on - strand, within Pf1N1B4_4245 at 4705.826 kb on - strand, within Pf1N1B4_4245 at 4705.849 kb on + strand, within Pf1N1B4_4245 at 4705.849 kb on + strand, within Pf1N1B4_4245 at 4705.849 kb on + strand, within Pf1N1B4_4245 at 4705.849 kb on + strand, within Pf1N1B4_4245 at 4705.850 kb on - strand, within Pf1N1B4_4245 at 4705.850 kb on - strand, within Pf1N1B4_4245 at 4705.867 kb on + strand, within Pf1N1B4_4245 at 4705.867 kb on + strand at 4705.868 kb on - strand, within Pf1N1B4_4245 at 4706.141 kb on - strand, within Pf1N1B4_4245 at 4706.141 kb on - strand, within Pf1N1B4_4245 at 4706.349 kb on + strand at 4706.349 kb on + strand at 4706.350 kb on - strand at 4706.350 kb on - strand at 4706.431 kb on + strand at 4706.431 kb on + strand at 4706.431 kb on + strand at 4706.432 kb on - strand at 4706.432 kb on - strand at 4706.577 kb on + strand at 4706.577 kb on + strand at 4706.577 kb on + strand at 4706.577 kb on + strand at 4706.577 kb on + strand at 4706.577 kb on + strand at 4706.578 kb on - strand at 4707.015 kb on + strand, within Pf1N1B4_4246 at 4707.016 kb on - strand, within Pf1N1B4_4246 at 4707.117 kb on + strand, within Pf1N1B4_4246 at 4707.117 kb on + strand, within Pf1N1B4_4246 at 4707.118 kb on - strand, within Pf1N1B4_4246 at 4707.118 kb on - strand, within Pf1N1B4_4246 at 4707.121 kb on + strand, within Pf1N1B4_4246 at 4707.122 kb on - strand, within Pf1N1B4_4246 at 4707.567 kb on + strand, within Pf1N1B4_4246 at 4707.568 kb on - strand, within Pf1N1B4_4246 at 4707.805 kb on + strand, within Pf1N1B4_4246 at 4708.250 kb on + strand at 4708.250 kb on + strand at 4708.311 kb on + strand at 4708.355 kb on + strand at 4708.355 kb on + strand at 4708.357 kb on + strand at 4708.358 kb on - strand at 4708.577 kb on + strand at 4708.578 kb on - strand at 4708.629 kb on + strand at 4708.630 kb on - strand at 4708.674 kb on + strand at 4708.674 kb on + strand at 4708.744 kb on + strand at 4708.775 kb on + strand at 4708.775 kb on + strand at 4708.775 kb on + strand at 4708.787 kb on - strand at 4708.812 kb on + strand at 4708.812 kb on + strand at 4708.813 kb on - strand at 4708.818 kb on + strand at 4708.823 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 4,705,786 + Pf1N1B4_4245 0.62 +0.4 4,705,787 - Pf1N1B4_4245 0.62 -0.5 4,705,798 + Pf1N1B4_4245 0.63 -0.2 4,705,798 + Pf1N1B4_4245 0.63 -0.8 4,705,826 - Pf1N1B4_4245 0.65 -1.2 4,705,826 - Pf1N1B4_4245 0.65 -0.8 4,705,849 + Pf1N1B4_4245 0.66 +1.2 4,705,849 + Pf1N1B4_4245 0.66 -1.0 4,705,849 + Pf1N1B4_4245 0.66 -0.7 4,705,849 + Pf1N1B4_4245 0.66 -0.0 4,705,850 - Pf1N1B4_4245 0.67 -1.2 4,705,850 - Pf1N1B4_4245 0.67 +1.4 4,705,867 + Pf1N1B4_4245 0.68 +0.6 4,705,867 + -0.6 4,705,868 - Pf1N1B4_4245 0.68 -1.6 4,706,141 - Pf1N1B4_4245 0.88 -0.5 4,706,141 - Pf1N1B4_4245 0.88 +0.4 4,706,349 + +1.4 4,706,349 + +0.0 4,706,350 - +1.0 4,706,350 - -0.0 4,706,431 + -1.0 4,706,431 + +2.0 4,706,431 + +1.3 4,706,432 - -0.9 4,706,432 - +0.2 4,706,577 + +0.1 4,706,577 + -0.3 4,706,577 + +0.2 4,706,577 + -1.2 4,706,577 + -1.2 4,706,577 + +0.4 4,706,578 - +1.1 4,707,015 + Pf1N1B4_4246 0.27 -2.2 4,707,016 - Pf1N1B4_4246 0.27 -1.5 4,707,117 + Pf1N1B4_4246 0.33 +0.6 4,707,117 + Pf1N1B4_4246 0.33 -0.8 4,707,118 - Pf1N1B4_4246 0.33 -1.6 4,707,118 - Pf1N1B4_4246 0.33 -1.4 4,707,121 + Pf1N1B4_4246 0.33 +0.7 4,707,122 - Pf1N1B4_4246 0.33 +0.9 4,707,567 + Pf1N1B4_4246 0.59 -2.6 4,707,568 - Pf1N1B4_4246 0.59 -1.2 4,707,805 + Pf1N1B4_4246 0.72 +1.4 4,708,250 + +0.7 4,708,250 + -0.8 4,708,311 + +0.0 4,708,355 + -1.6 4,708,355 + -0.5 4,708,357 + -0.3 4,708,358 - -2.2 4,708,577 + -0.4 4,708,578 - +0.6 4,708,629 + +1.6 4,708,630 - -2.4 4,708,674 + +0.9 4,708,674 + +1.6 4,708,744 + -1.2 4,708,775 + +1.4 4,708,775 + +0.4 4,708,775 + +1.2 4,708,787 - +1.6 4,708,812 + +0.1 4,708,812 + +0.4 4,708,813 - +0.3 4,708,818 + +1.6 4,708,823 + +0.9
Or see this region's nucleotide sequence