Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4221 and Pf1N1B4_4222 are separated by 19 nucleotides Pf1N1B4_4222 and Pf1N1B4_4223 are separated by 6 nucleotides
Pf1N1B4_4221 - hypothetical protein, at 4,678,383 to 4,678,499
_4221
Pf1N1B4_4222 - putative tyrosinase, at 4,678,519 to 4,679,379
_4222
Pf1N1B4_4223 - Transcriptional regulator, at 4,679,386 to 4,680,279
_4223
Position (kb)
4678
4679 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4677.459 kb on + strand at 4677.517 kb on - strand at 4677.553 kb on - strand at 4677.604 kb on + strand at 4677.671 kb on - strand at 4677.831 kb on + strand at 4677.831 kb on + strand at 4677.835 kb on + strand at 4677.836 kb on - strand at 4677.978 kb on + strand at 4677.979 kb on - strand at 4678.073 kb on + strand at 4678.108 kb on + strand at 4678.108 kb on + strand at 4678.108 kb on + strand at 4678.108 kb on + strand at 4678.108 kb on + strand at 4678.108 kb on + strand at 4678.109 kb on - strand at 4678.126 kb on + strand at 4678.269 kb on + strand at 4678.270 kb on - strand at 4678.362 kb on + strand at 4678.497 kb on + strand at 4678.497 kb on + strand at 4678.497 kb on + strand at 4678.497 kb on + strand at 4678.498 kb on - strand at 4678.531 kb on + strand at 4678.604 kb on - strand at 4678.604 kb on - strand at 4678.846 kb on + strand, within Pf1N1B4_4222 at 4678.846 kb on + strand, within Pf1N1B4_4222 at 4679.011 kb on + strand, within Pf1N1B4_4222 at 4679.012 kb on - strand, within Pf1N1B4_4222 at 4679.027 kb on - strand, within Pf1N1B4_4222 at 4679.027 kb on - strand, within Pf1N1B4_4222 at 4679.027 kb on - strand, within Pf1N1B4_4222 at 4679.098 kb on - strand, within Pf1N1B4_4222 at 4679.230 kb on + strand, within Pf1N1B4_4222 at 4679.231 kb on - strand at 4679.234 kb on + strand, within Pf1N1B4_4222 at 4679.234 kb on + strand, within Pf1N1B4_4222 at 4679.235 kb on - strand, within Pf1N1B4_4222 at 4679.235 kb on - strand, within Pf1N1B4_4222 at 4679.290 kb on + strand, within Pf1N1B4_4222 at 4679.290 kb on + strand, within Pf1N1B4_4222 at 4679.291 kb on - strand, within Pf1N1B4_4222 at 4679.291 kb on - strand, within Pf1N1B4_4222 at 4679.291 kb on - strand, within Pf1N1B4_4222 at 4679.335 kb on + strand at 4679.336 kb on - strand at 4679.345 kb on - strand at 4679.347 kb on + strand at 4679.347 kb on + strand at 4679.347 kb on + strand at 4679.347 kb on + strand at 4679.348 kb on - strand at 4679.348 kb on - strand at 4679.348 kb on - strand at 4679.353 kb on + strand at 4679.377 kb on + strand at 4679.377 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 4,677,459 + +0.5 4,677,517 - +1.5 4,677,553 - +2.3 4,677,604 + +1.5 4,677,671 - +0.0 4,677,831 + -0.5 4,677,831 + -0.2 4,677,835 + +0.9 4,677,836 - +0.1 4,677,978 + -0.2 4,677,979 - +1.0 4,678,073 + +1.6 4,678,108 + -0.1 4,678,108 + +1.5 4,678,108 + +1.0 4,678,108 + -0.1 4,678,108 + +1.3 4,678,108 + +0.5 4,678,109 - +0.7 4,678,126 + -0.2 4,678,269 + +1.2 4,678,270 - +0.5 4,678,362 + -0.0 4,678,497 + -0.5 4,678,497 + +0.8 4,678,497 + +0.1 4,678,497 + -0.9 4,678,498 - -0.5 4,678,531 + -0.9 4,678,604 - -1.7 4,678,604 - +0.2 4,678,846 + Pf1N1B4_4222 0.38 +0.8 4,678,846 + Pf1N1B4_4222 0.38 +0.5 4,679,011 + Pf1N1B4_4222 0.57 -1.4 4,679,012 - Pf1N1B4_4222 0.57 -0.0 4,679,027 - Pf1N1B4_4222 0.59 +0.2 4,679,027 - Pf1N1B4_4222 0.59 +2.5 4,679,027 - Pf1N1B4_4222 0.59 +1.6 4,679,098 - Pf1N1B4_4222 0.67 +1.5 4,679,230 + Pf1N1B4_4222 0.83 +1.0 4,679,231 - -0.5 4,679,234 + Pf1N1B4_4222 0.83 -0.1 4,679,234 + Pf1N1B4_4222 0.83 -2.7 4,679,235 - Pf1N1B4_4222 0.83 +2.0 4,679,235 - Pf1N1B4_4222 0.83 -0.5 4,679,290 + Pf1N1B4_4222 0.90 +0.5 4,679,290 + Pf1N1B4_4222 0.90 -1.1 4,679,291 - Pf1N1B4_4222 0.90 +0.0 4,679,291 - Pf1N1B4_4222 0.90 -0.5 4,679,291 - Pf1N1B4_4222 0.90 +2.2 4,679,335 + -3.0 4,679,336 - -0.4 4,679,345 - -0.2 4,679,347 + +0.5 4,679,347 + +1.1 4,679,347 + -0.1 4,679,347 + +0.0 4,679,348 - -1.1 4,679,348 - -2.9 4,679,348 - +0.0 4,679,353 + +2.0 4,679,377 + +1.2 4,679,377 + +0.5
Or see this region's nucleotide sequence