Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4179 and Pf1N1B4_4180 are separated by 163 nucleotides Pf1N1B4_4180 and Pf1N1B4_4181 are separated by 45 nucleotides Pf1N1B4_4181 and Pf1N1B4_4182 are separated by 112 nucleotides
Pf1N1B4_4179 - phosphoribosyltransferase, at 4,628,530 to 4,629,114
_4179
Pf1N1B4_4180 - Phosphoribosyl transferase domain protein, at 4,629,278 to 4,629,955
_4180
Pf1N1B4_4181 - FIG00957123: hypothetical protein, at 4,630,001 to 4,630,426
_4181
Pf1N1B4_4182 - Universal stress protein family 5, at 4,630,539 to 4,631,459
_4182
Position (kb) 4630
4631 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 4629.149 kb on + strand at 4629.149 kb on + strand at 4629.456 kb on + strand, within Pf1N1B4_4180 at 4629.457 kb on - strand, within Pf1N1B4_4180 at 4629.457 kb on - strand, within Pf1N1B4_4180 at 4629.784 kb on + strand, within Pf1N1B4_4180 at 4629.784 kb on + strand, within Pf1N1B4_4180 at 4629.926 kb on - strand at 4629.966 kb on - strand at 4630.003 kb on - strand at 4630.018 kb on - strand at 4630.064 kb on + strand, within Pf1N1B4_4181 at 4630.065 kb on - strand, within Pf1N1B4_4181 at 4630.165 kb on - strand, within Pf1N1B4_4181 at 4630.165 kb on - strand at 4630.236 kb on + strand, within Pf1N1B4_4181 at 4630.237 kb on - strand at 4630.266 kb on - strand, within Pf1N1B4_4181 at 4630.327 kb on + strand, within Pf1N1B4_4181 at 4630.377 kb on - strand, within Pf1N1B4_4181 at 4630.402 kb on + strand at 4630.468 kb on + strand at 4630.468 kb on + strand at 4630.469 kb on - strand at 4630.669 kb on + strand, within Pf1N1B4_4182 at 4630.670 kb on - strand, within Pf1N1B4_4182 at 4630.670 kb on - strand, within Pf1N1B4_4182 at 4630.670 kb on - strand, within Pf1N1B4_4182 at 4630.740 kb on - strand, within Pf1N1B4_4182 at 4630.876 kb on + strand, within Pf1N1B4_4182 at 4630.877 kb on - strand, within Pf1N1B4_4182 at 4630.877 kb on - strand at 4630.877 kb on - strand, within Pf1N1B4_4182 at 4630.877 kb on - strand, within Pf1N1B4_4182 at 4630.877 kb on - strand, within Pf1N1B4_4182 at 4630.880 kb on + strand at 4630.880 kb on + strand, within Pf1N1B4_4182 at 4630.881 kb on - strand, within Pf1N1B4_4182 at 4630.881 kb on - strand, within Pf1N1B4_4182 at 4630.904 kb on + strand, within Pf1N1B4_4182 at 4630.954 kb on + strand, within Pf1N1B4_4182 at 4630.954 kb on + strand at 4630.955 kb on - strand, within Pf1N1B4_4182 at 4630.955 kb on - strand, within Pf1N1B4_4182 at 4631.108 kb on - strand, within Pf1N1B4_4182 at 4631.108 kb on - strand, within Pf1N1B4_4182 at 4631.108 kb on - strand, within Pf1N1B4_4182 at 4631.219 kb on + strand, within Pf1N1B4_4182
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 4,629,149 + +0.7 4,629,149 + +0.3 4,629,456 + Pf1N1B4_4180 0.26 -0.1 4,629,457 - Pf1N1B4_4180 0.26 -0.9 4,629,457 - Pf1N1B4_4180 0.26 +0.5 4,629,784 + Pf1N1B4_4180 0.75 +0.3 4,629,784 + Pf1N1B4_4180 0.75 -0.1 4,629,926 - -1.1 4,629,966 - -0.5 4,630,003 - +1.3 4,630,018 - -0.1 4,630,064 + Pf1N1B4_4181 0.15 -2.9 4,630,065 - Pf1N1B4_4181 0.15 -1.3 4,630,165 - Pf1N1B4_4181 0.38 -1.5 4,630,165 - +1.2 4,630,236 + Pf1N1B4_4181 0.55 -2.3 4,630,237 - +2.8 4,630,266 - Pf1N1B4_4181 0.62 -0.3 4,630,327 + Pf1N1B4_4181 0.77 -0.5 4,630,377 - Pf1N1B4_4181 0.88 +0.5 4,630,402 + -0.9 4,630,468 + +0.5 4,630,468 + +0.5 4,630,469 - -0.5 4,630,669 + Pf1N1B4_4182 0.14 -0.7 4,630,670 - Pf1N1B4_4182 0.14 -0.3 4,630,670 - Pf1N1B4_4182 0.14 -1.5 4,630,670 - Pf1N1B4_4182 0.14 -0.3 4,630,740 - Pf1N1B4_4182 0.22 +0.5 4,630,876 + Pf1N1B4_4182 0.37 +0.9 4,630,877 - Pf1N1B4_4182 0.37 -0.2 4,630,877 - -0.5 4,630,877 - Pf1N1B4_4182 0.37 +1.1 4,630,877 - Pf1N1B4_4182 0.37 +0.1 4,630,877 - Pf1N1B4_4182 0.37 -0.9 4,630,880 + +0.5 4,630,880 + Pf1N1B4_4182 0.37 +0.5 4,630,881 - Pf1N1B4_4182 0.37 +0.6 4,630,881 - Pf1N1B4_4182 0.37 -0.2 4,630,904 + Pf1N1B4_4182 0.40 -0.9 4,630,954 + Pf1N1B4_4182 0.45 -0.1 4,630,954 + -0.5 4,630,955 - Pf1N1B4_4182 0.45 +0.9 4,630,955 - Pf1N1B4_4182 0.45 +1.1 4,631,108 - Pf1N1B4_4182 0.62 -2.0 4,631,108 - Pf1N1B4_4182 0.62 -0.1 4,631,108 - Pf1N1B4_4182 0.62 +0.4 4,631,219 + Pf1N1B4_4182 0.74 +1.8
Or see this region's nucleotide sequence