Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4009 and Pf1N1B4_4010 overlap by 4 nucleotides Pf1N1B4_4010 and Pf1N1B4_4011 are separated by 8 nucleotides Pf1N1B4_4011 and Pf1N1B4_4012 are separated by 10 nucleotides Pf1N1B4_4012 and Pf1N1B4_4013 are separated by 48 nucleotides
Pf1N1B4_4009 - Glycerol-3-phosphate ABC transporter, ATP-binding protein UgpC (TC 3.A.1.1.3), at 4,460,568 to 4,461,665
_4009
Pf1N1B4_4010 - Glycerol-3-phosphate ABC transporter, permease protein UgpA (TC 3.A.1.1.3), at 4,461,662 to 4,462,528
_4010
Pf1N1B4_4011 - Glycerol-3-phosphate ABC transporter, permease protein UgpE (TC 3.A.1.1.3), at 4,462,537 to 4,463,337
_4011
Pf1N1B4_4012 - Probable transmembrane protein, at 4,463,348 to 4,463,620
_4012
Pf1N1B4_4013 - Glycerol-3-phosphate ABC transporter, permease protein UgpA (TC 3.A.1.1.3), at 4,463,669 to 4,465,411
_4013
Position (kb)
4462
4463
4464 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4461.556 kb on + strand at 4461.597 kb on + strand at 4461.597 kb on + strand at 4461.597 kb on + strand at 4461.598 kb on - strand at 4461.598 kb on - strand at 4461.598 kb on - strand at 4461.598 kb on - strand at 4461.598 kb on - strand at 4461.630 kb on + strand at 4461.761 kb on + strand, within Pf1N1B4_4010 at 4461.818 kb on + strand, within Pf1N1B4_4010 at 4461.819 kb on - strand, within Pf1N1B4_4010 at 4461.940 kb on + strand, within Pf1N1B4_4010 at 4462.085 kb on + strand, within Pf1N1B4_4010 at 4462.085 kb on + strand, within Pf1N1B4_4010 at 4462.085 kb on + strand, within Pf1N1B4_4010 at 4462.085 kb on + strand, within Pf1N1B4_4010 at 4462.085 kb on + strand, within Pf1N1B4_4010 at 4462.086 kb on - strand, within Pf1N1B4_4010 at 4462.086 kb on - strand, within Pf1N1B4_4010 at 4462.086 kb on - strand at 4462.091 kb on + strand, within Pf1N1B4_4010 at 4462.091 kb on + strand, within Pf1N1B4_4010 at 4462.091 kb on + strand, within Pf1N1B4_4010 at 4462.091 kb on + strand, within Pf1N1B4_4010 at 4462.091 kb on + strand, within Pf1N1B4_4010 at 4462.091 kb on + strand, within Pf1N1B4_4010 at 4462.091 kb on + strand, within Pf1N1B4_4010 at 4462.092 kb on - strand, within Pf1N1B4_4010 at 4462.205 kb on + strand, within Pf1N1B4_4010 at 4462.206 kb on - strand, within Pf1N1B4_4010 at 4462.206 kb on - strand, within Pf1N1B4_4010 at 4462.209 kb on - strand, within Pf1N1B4_4010 at 4462.457 kb on + strand at 4462.457 kb on + strand at 4462.458 kb on - strand at 4462.494 kb on - strand at 4462.566 kb on - strand at 4462.571 kb on - strand at 4462.571 kb on - strand at 4462.598 kb on - strand at 4462.685 kb on - strand, within Pf1N1B4_4011 at 4462.714 kb on + strand, within Pf1N1B4_4011 at 4462.738 kb on + strand, within Pf1N1B4_4011 at 4462.741 kb on + strand, within Pf1N1B4_4011 at 4462.742 kb on - strand, within Pf1N1B4_4011 at 4462.742 kb on - strand, within Pf1N1B4_4011 at 4462.792 kb on + strand, within Pf1N1B4_4011 at 4462.900 kb on + strand, within Pf1N1B4_4011 at 4462.900 kb on + strand, within Pf1N1B4_4011 at 4462.900 kb on + strand, within Pf1N1B4_4011 at 4462.900 kb on + strand, within Pf1N1B4_4011 at 4462.900 kb on + strand, within Pf1N1B4_4011 at 4463.033 kb on - strand, within Pf1N1B4_4011 at 4463.523 kb on - strand, within Pf1N1B4_4012 at 4463.668 kb on + strand at 4463.668 kb on + strand at 4463.668 kb on + strand at 4463.668 kb on + strand at 4463.669 kb on - strand at 4463.698 kb on + strand at 4463.698 kb on + strand at 4463.698 kb on + strand at 4463.699 kb on - strand at 4463.762 kb on + strand at 4463.763 kb on - strand at 4464.047 kb on + strand, within Pf1N1B4_4013 at 4464.047 kb on + strand, within Pf1N1B4_4013 at 4464.048 kb on - strand, within Pf1N1B4_4013 at 4464.048 kb on - strand at 4464.048 kb on - strand, within Pf1N1B4_4013 at 4464.147 kb on - strand, within Pf1N1B4_4013 at 4464.218 kb on + strand, within Pf1N1B4_4013 at 4464.219 kb on - strand, within Pf1N1B4_4013 at 4464.251 kb on + strand, within Pf1N1B4_4013 at 4464.312 kb on - strand, within Pf1N1B4_4013 at 4464.330 kb on + strand, within Pf1N1B4_4013
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 4,461,556 + -0.3 4,461,597 + -1.0 4,461,597 + -0.0 4,461,597 + +1.0 4,461,598 - -2.4 4,461,598 - +0.6 4,461,598 - +0.2 4,461,598 - +0.1 4,461,598 - -0.3 4,461,630 + -1.2 4,461,761 + Pf1N1B4_4010 0.11 +1.0 4,461,818 + Pf1N1B4_4010 0.18 +0.2 4,461,819 - Pf1N1B4_4010 0.18 +2.2 4,461,940 + Pf1N1B4_4010 0.32 -1.2 4,462,085 + Pf1N1B4_4010 0.49 -0.4 4,462,085 + Pf1N1B4_4010 0.49 -0.4 4,462,085 + Pf1N1B4_4010 0.49 +0.2 4,462,085 + Pf1N1B4_4010 0.49 +0.6 4,462,085 + Pf1N1B4_4010 0.49 +0.6 4,462,086 - Pf1N1B4_4010 0.49 +0.6 4,462,086 - Pf1N1B4_4010 0.49 -0.2 4,462,086 - -0.0 4,462,091 + Pf1N1B4_4010 0.49 -0.2 4,462,091 + Pf1N1B4_4010 0.49 -0.4 4,462,091 + Pf1N1B4_4010 0.49 +1.3 4,462,091 + Pf1N1B4_4010 0.49 -0.0 4,462,091 + Pf1N1B4_4010 0.49 -0.3 4,462,091 + Pf1N1B4_4010 0.49 -0.5 4,462,091 + Pf1N1B4_4010 0.49 +1.1 4,462,092 - Pf1N1B4_4010 0.50 -1.2 4,462,205 + Pf1N1B4_4010 0.63 +0.4 4,462,206 - Pf1N1B4_4010 0.63 -1.2 4,462,206 - Pf1N1B4_4010 0.63 +0.9 4,462,209 - Pf1N1B4_4010 0.63 +0.0 4,462,457 + +1.6 4,462,457 + +0.9 4,462,458 - -1.0 4,462,494 - +0.6 4,462,566 - +0.1 4,462,571 - +0.6 4,462,571 - -1.0 4,462,598 - +0.3 4,462,685 - Pf1N1B4_4011 0.18 -1.0 4,462,714 + Pf1N1B4_4011 0.22 -0.6 4,462,738 + Pf1N1B4_4011 0.25 -1.7 4,462,741 + Pf1N1B4_4011 0.25 -0.4 4,462,742 - Pf1N1B4_4011 0.26 -0.3 4,462,742 - Pf1N1B4_4011 0.26 -1.0 4,462,792 + Pf1N1B4_4011 0.32 +1.6 4,462,900 + Pf1N1B4_4011 0.45 -1.0 4,462,900 + Pf1N1B4_4011 0.45 -0.8 4,462,900 + Pf1N1B4_4011 0.45 -0.7 4,462,900 + Pf1N1B4_4011 0.45 -0.7 4,462,900 + Pf1N1B4_4011 0.45 -0.4 4,463,033 - Pf1N1B4_4011 0.62 -0.2 4,463,523 - Pf1N1B4_4012 0.64 +0.8 4,463,668 + +0.3 4,463,668 + -0.0 4,463,668 + +0.6 4,463,668 + +0.6 4,463,669 - -1.7 4,463,698 + +0.9 4,463,698 + -1.7 4,463,698 + +2.9 4,463,699 - +1.2 4,463,762 + +0.3 4,463,763 - +0.8 4,464,047 + Pf1N1B4_4013 0.22 +0.8 4,464,047 + Pf1N1B4_4013 0.22 +0.9 4,464,048 - Pf1N1B4_4013 0.22 -0.7 4,464,048 - +0.2 4,464,048 - Pf1N1B4_4013 0.22 -1.0 4,464,147 - Pf1N1B4_4013 0.27 +1.4 4,464,218 + Pf1N1B4_4013 0.31 -1.6 4,464,219 - Pf1N1B4_4013 0.32 -0.6 4,464,251 + Pf1N1B4_4013 0.33 +1.3 4,464,312 - Pf1N1B4_4013 0.37 +0.2 4,464,330 + Pf1N1B4_4013 0.38 -1.0
Or see this region's nucleotide sequence