Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3744 and Pf1N1B4_3745 overlap by 4 nucleotides Pf1N1B4_3745 and Pf1N1B4_3746 overlap by 14 nucleotides Pf1N1B4_3746 and Pf1N1B4_3747 overlap by 4 nucleotides
Pf1N1B4_3744 - ABC transporter, ATP-binding protein, at 4,155,693 to 4,156,682
_3744
Pf1N1B4_3745 - Thiamin ABC transporter, transmembrane component, at 4,156,679 to 4,157,479
_3745
Pf1N1B4_3746 - ABC transporter permease protein, at 4,157,466 to 4,158,311
_3746
Pf1N1B4_3747 - FIG00956482: hypothetical protein, at 4,158,308 to 4,159,114
_3747
Position (kb)
4156
4157
4158 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4155.973 kb on + strand, within Pf1N1B4_3744 at 4155.973 kb on + strand, within Pf1N1B4_3744 at 4155.973 kb on + strand, within Pf1N1B4_3744 at 4155.973 kb on + strand, within Pf1N1B4_3744 at 4155.973 kb on + strand, within Pf1N1B4_3744 at 4155.974 kb on - strand, within Pf1N1B4_3744 at 4156.018 kb on + strand, within Pf1N1B4_3744 at 4156.018 kb on + strand, within Pf1N1B4_3744 at 4156.018 kb on + strand, within Pf1N1B4_3744 at 4156.019 kb on - strand, within Pf1N1B4_3744 at 4156.019 kb on - strand, within Pf1N1B4_3744 at 4156.044 kb on + strand, within Pf1N1B4_3744 at 4156.044 kb on + strand, within Pf1N1B4_3744 at 4156.044 kb on + strand at 4156.045 kb on - strand at 4156.433 kb on - strand, within Pf1N1B4_3744 at 4156.630 kb on - strand at 4156.630 kb on - strand at 4156.634 kb on + strand at 4156.634 kb on + strand at 4156.635 kb on - strand at 4156.635 kb on - strand at 4156.675 kb on + strand at 4156.676 kb on - strand at 4156.676 kb on - strand at 4156.676 kb on - strand at 4156.706 kb on - strand at 4156.706 kb on - strand at 4156.743 kb on + strand at 4156.783 kb on + strand, within Pf1N1B4_3745 at 4156.792 kb on + strand, within Pf1N1B4_3745 at 4156.792 kb on + strand, within Pf1N1B4_3745 at 4156.801 kb on - strand, within Pf1N1B4_3745 at 4157.049 kb on + strand, within Pf1N1B4_3745 at 4157.049 kb on + strand, within Pf1N1B4_3745 at 4157.050 kb on - strand, within Pf1N1B4_3745 at 4157.050 kb on - strand at 4157.320 kb on - strand, within Pf1N1B4_3745 at 4157.332 kb on + strand, within Pf1N1B4_3745 at 4157.379 kb on + strand, within Pf1N1B4_3745 at 4157.380 kb on - strand, within Pf1N1B4_3745 at 4157.427 kb on + strand at 4157.427 kb on + strand at 4157.427 kb on + strand at 4157.427 kb on + strand at 4157.427 kb on + strand at 4157.428 kb on - strand at 4157.446 kb on - strand at 4157.449 kb on + strand at 4157.482 kb on + strand at 4157.482 kb on + strand at 4157.482 kb on + strand at 4157.483 kb on - strand at 4157.647 kb on + strand, within Pf1N1B4_3746 at 4157.689 kb on - strand, within Pf1N1B4_3746 at 4157.848 kb on + strand, within Pf1N1B4_3746 at 4157.849 kb on - strand, within Pf1N1B4_3746 at 4157.849 kb on - strand, within Pf1N1B4_3746 at 4157.854 kb on + strand, within Pf1N1B4_3746 at 4157.854 kb on + strand, within Pf1N1B4_3746 at 4157.855 kb on - strand, within Pf1N1B4_3746 at 4157.855 kb on - strand, within Pf1N1B4_3746 at 4157.855 kb on - strand, within Pf1N1B4_3746 at 4158.137 kb on - strand, within Pf1N1B4_3746 at 4158.137 kb on - strand, within Pf1N1B4_3746 at 4158.137 kb on - strand, within Pf1N1B4_3746 at 4158.291 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 4,155,973 + Pf1N1B4_3744 0.28 -1.7 4,155,973 + Pf1N1B4_3744 0.28 +0.8 4,155,973 + Pf1N1B4_3744 0.28 -0.2 4,155,973 + Pf1N1B4_3744 0.28 -0.7 4,155,973 + Pf1N1B4_3744 0.28 -0.4 4,155,974 - Pf1N1B4_3744 0.28 -0.9 4,156,018 + Pf1N1B4_3744 0.33 -1.6 4,156,018 + Pf1N1B4_3744 0.33 -0.5 4,156,018 + Pf1N1B4_3744 0.33 -0.7 4,156,019 - Pf1N1B4_3744 0.33 -1.5 4,156,019 - Pf1N1B4_3744 0.33 -1.4 4,156,044 + Pf1N1B4_3744 0.35 +0.2 4,156,044 + Pf1N1B4_3744 0.35 +0.1 4,156,044 + +0.6 4,156,045 - -0.9 4,156,433 - Pf1N1B4_3744 0.75 +0.9 4,156,630 - -0.4 4,156,630 - +0.6 4,156,634 + +1.0 4,156,634 + -0.2 4,156,635 - +0.8 4,156,635 - -0.0 4,156,675 + +0.9 4,156,676 - +0.1 4,156,676 - -0.1 4,156,676 - +0.3 4,156,706 - -1.7 4,156,706 - +1.2 4,156,743 + +0.6 4,156,783 + Pf1N1B4_3745 0.13 +0.3 4,156,792 + Pf1N1B4_3745 0.14 -0.4 4,156,792 + Pf1N1B4_3745 0.14 -0.9 4,156,801 - Pf1N1B4_3745 0.15 -0.4 4,157,049 + Pf1N1B4_3745 0.46 -1.7 4,157,049 + Pf1N1B4_3745 0.46 -1.9 4,157,050 - Pf1N1B4_3745 0.46 -1.7 4,157,050 - -0.9 4,157,320 - Pf1N1B4_3745 0.80 -2.5 4,157,332 + Pf1N1B4_3745 0.82 -0.4 4,157,379 + Pf1N1B4_3745 0.87 +0.1 4,157,380 - Pf1N1B4_3745 0.88 +0.6 4,157,427 + +3.2 4,157,427 + -0.4 4,157,427 + +0.6 4,157,427 + +0.6 4,157,427 + +0.3 4,157,428 - +0.1 4,157,446 - +0.2 4,157,449 + -1.5 4,157,482 + +1.2 4,157,482 + +0.6 4,157,482 + -0.4 4,157,483 - +0.1 4,157,647 + Pf1N1B4_3746 0.21 +0.9 4,157,689 - Pf1N1B4_3746 0.26 +1.1 4,157,848 + Pf1N1B4_3746 0.45 +1.2 4,157,849 - Pf1N1B4_3746 0.45 +0.9 4,157,849 - Pf1N1B4_3746 0.45 +2.2 4,157,854 + Pf1N1B4_3746 0.46 +1.6 4,157,854 + Pf1N1B4_3746 0.46 -0.4 4,157,855 - Pf1N1B4_3746 0.46 -2.5 4,157,855 - Pf1N1B4_3746 0.46 -1.8 4,157,855 - Pf1N1B4_3746 0.46 +0.2 4,158,137 - Pf1N1B4_3746 0.79 -0.7 4,158,137 - Pf1N1B4_3746 0.79 -0.4 4,158,137 - Pf1N1B4_3746 0.79 -0.8 4,158,291 - +0.5
Or see this region's nucleotide sequence