Experiment: Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3728 and Pf1N1B4_3729 are separated by 125 nucleotides Pf1N1B4_3729 and Pf1N1B4_3730 are separated by 120 nucleotides
Pf1N1B4_3728 - 1-acyl-sn-glycerol-3-phosphate acyltransferase (EC 2.3.1.51), at 4,137,283 to 4,139,157
_3728
Pf1N1B4_3729 - Quaternary ammonium compound-resistance protein SugE, at 4,139,283 to 4,139,597
_3729
Pf1N1B4_3730 - Sodium-dependent transporter, at 4,139,718 to 4,140,683
_3730
Position (kb)
4139
4140 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4138.351 kb on + strand, within Pf1N1B4_3728 at 4138.351 kb on + strand, within Pf1N1B4_3728 at 4138.351 kb on + strand, within Pf1N1B4_3728 at 4138.352 kb on - strand, within Pf1N1B4_3728 at 4138.371 kb on + strand, within Pf1N1B4_3728 at 4138.371 kb on + strand at 4138.372 kb on - strand at 4138.496 kb on + strand, within Pf1N1B4_3728 at 4138.496 kb on + strand, within Pf1N1B4_3728 at 4138.497 kb on - strand, within Pf1N1B4_3728 at 4138.537 kb on + strand, within Pf1N1B4_3728 at 4138.537 kb on + strand, within Pf1N1B4_3728 at 4138.537 kb on + strand, within Pf1N1B4_3728 at 4138.537 kb on + strand, within Pf1N1B4_3728 at 4138.537 kb on + strand, within Pf1N1B4_3728 at 4138.537 kb on + strand, within Pf1N1B4_3728 at 4138.537 kb on + strand, within Pf1N1B4_3728 at 4138.538 kb on - strand, within Pf1N1B4_3728 at 4138.709 kb on + strand, within Pf1N1B4_3728 at 4138.709 kb on + strand, within Pf1N1B4_3728 at 4138.735 kb on + strand, within Pf1N1B4_3728 at 4138.735 kb on + strand, within Pf1N1B4_3728 at 4138.739 kb on - strand, within Pf1N1B4_3728 at 4138.747 kb on + strand, within Pf1N1B4_3728 at 4138.828 kb on + strand, within Pf1N1B4_3728 at 4139.129 kb on + strand at 4139.155 kb on + strand at 4139.225 kb on + strand at 4139.225 kb on + strand at 4139.226 kb on - strand at 4139.285 kb on - strand at 4139.423 kb on - strand at 4139.423 kb on - strand, within Pf1N1B4_3729 at 4139.533 kb on + strand, within Pf1N1B4_3729 at 4139.533 kb on + strand, within Pf1N1B4_3729 at 4139.533 kb on + strand, within Pf1N1B4_3729 at 4139.534 kb on - strand, within Pf1N1B4_3729 at 4139.534 kb on - strand, within Pf1N1B4_3729 at 4139.534 kb on - strand at 4139.678 kb on + strand at 4139.679 kb on - strand at 4139.679 kb on - strand at 4139.679 kb on - strand at 4139.679 kb on - strand at 4139.679 kb on - strand at 4139.711 kb on + strand at 4139.711 kb on + strand at 4139.712 kb on - strand at 4139.719 kb on + strand at 4139.719 kb on + strand at 4139.768 kb on + strand at 4139.779 kb on + strand at 4139.779 kb on + strand at 4139.779 kb on + strand at 4139.780 kb on - strand at 4139.962 kb on + strand, within Pf1N1B4_3730 at 4139.962 kb on + strand, within Pf1N1B4_3730 at 4139.962 kb on + strand, within Pf1N1B4_3730 at 4139.974 kb on + strand, within Pf1N1B4_3730 at 4139.975 kb on - strand, within Pf1N1B4_3730 at 4140.462 kb on + strand, within Pf1N1B4_3730 at 4140.463 kb on - strand, within Pf1N1B4_3730 at 4140.562 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml remove 4,138,351 + Pf1N1B4_3728 0.57 +0.3 4,138,351 + Pf1N1B4_3728 0.57 -0.9 4,138,351 + Pf1N1B4_3728 0.57 -1.1 4,138,352 - Pf1N1B4_3728 0.57 +0.4 4,138,371 + Pf1N1B4_3728 0.58 +0.9 4,138,371 + -1.7 4,138,372 - +1.6 4,138,496 + Pf1N1B4_3728 0.65 -0.4 4,138,496 + Pf1N1B4_3728 0.65 -0.4 4,138,497 - Pf1N1B4_3728 0.65 +0.4 4,138,537 + Pf1N1B4_3728 0.67 -1.2 4,138,537 + Pf1N1B4_3728 0.67 +0.6 4,138,537 + Pf1N1B4_3728 0.67 +0.6 4,138,537 + Pf1N1B4_3728 0.67 -1.9 4,138,537 + Pf1N1B4_3728 0.67 -0.9 4,138,537 + Pf1N1B4_3728 0.67 +0.2 4,138,537 + Pf1N1B4_3728 0.67 -0.4 4,138,538 - Pf1N1B4_3728 0.67 -0.7 4,138,709 + Pf1N1B4_3728 0.76 +0.6 4,138,709 + Pf1N1B4_3728 0.76 +0.5 4,138,735 + Pf1N1B4_3728 0.77 +0.3 4,138,735 + Pf1N1B4_3728 0.77 -0.1 4,138,739 - Pf1N1B4_3728 0.78 +0.5 4,138,747 + Pf1N1B4_3728 0.78 -1.7 4,138,828 + Pf1N1B4_3728 0.82 +0.8 4,139,129 + +0.1 4,139,155 + -0.9 4,139,225 + -0.9 4,139,225 + +0.5 4,139,226 - -0.4 4,139,285 - +0.3 4,139,423 - +1.2 4,139,423 - Pf1N1B4_3729 0.44 +0.4 4,139,533 + Pf1N1B4_3729 0.79 -0.4 4,139,533 + Pf1N1B4_3729 0.79 -0.6 4,139,533 + Pf1N1B4_3729 0.79 -1.1 4,139,534 - Pf1N1B4_3729 0.80 -1.4 4,139,534 - Pf1N1B4_3729 0.80 -0.9 4,139,534 - -0.4 4,139,678 + +0.6 4,139,679 - +0.1 4,139,679 - -0.2 4,139,679 - -0.6 4,139,679 - -1.5 4,139,679 - +0.1 4,139,711 + -0.9 4,139,711 + +2.0 4,139,712 - +1.1 4,139,719 + +0.6 4,139,719 + +0.6 4,139,768 + -0.4 4,139,779 + +0.3 4,139,779 + -1.4 4,139,779 + +0.5 4,139,780 - +0.3 4,139,962 + Pf1N1B4_3730 0.25 -0.9 4,139,962 + Pf1N1B4_3730 0.25 -1.2 4,139,962 + Pf1N1B4_3730 0.25 +0.3 4,139,974 + Pf1N1B4_3730 0.27 -0.0 4,139,975 - Pf1N1B4_3730 0.27 -0.8 4,140,462 + Pf1N1B4_3730 0.77 +0.2 4,140,463 - Pf1N1B4_3730 0.77 -0.4 4,140,562 - +0.6
Or see this region's nucleotide sequence