Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3609

Experiment: Spectinomycin 0.025 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3607 and Pf1N1B4_3608 are separated by 6 nucleotidesPf1N1B4_3608 and Pf1N1B4_3609 are separated by 218 nucleotidesPf1N1B4_3609 and Pf1N1B4_3610 are separated by 5 nucleotides Pf1N1B4_3607 - Flagellum-specific ATP synthase FliI, at 4,018,073 to 4,019,431 _3607 Pf1N1B4_3608 - Flagellar protein FliJ, at 4,019,438 to 4,019,887 _3608 Pf1N1B4_3609 - Anti-sigma F factor antagonist (spoIIAA-2); Anti-sigma B factor antagonist RsbV, at 4,020,106 to 4,020,411 _3609 Pf1N1B4_3610 - Serine phosphatase RsbU, regulator of sigma subunit, at 4,020,417 to 4,022,120 _3610 Position (kb) 4020 4021Strain fitness (log2 ratio) -2 -1 0 1 2at 4019.231 kb on + strand, within Pf1N1B4_3607at 4019.231 kb on + strand, within Pf1N1B4_3607at 4019.279 kb on + strand, within Pf1N1B4_3607at 4019.279 kb on + strand, within Pf1N1B4_3607at 4019.280 kb on - strand, within Pf1N1B4_3607at 4019.280 kb on - strand, within Pf1N1B4_3607at 4019.342 kb on + strandat 4019.342 kb on + strandat 4019.342 kb on + strandat 4019.342 kb on + strandat 4019.343 kb on - strandat 4019.343 kb on - strandat 4019.343 kb on - strandat 4019.343 kb on - strandat 4019.343 kb on - strandat 4019.343 kb on - strandat 4019.343 kb on - strandat 4019.343 kb on - strandat 4019.589 kb on - strand, within Pf1N1B4_3608at 4019.719 kb on + strand, within Pf1N1B4_3608at 4019.720 kb on - strand, within Pf1N1B4_3608at 4019.882 kb on + strandat 4019.934 kb on - strandat 4019.980 kb on - strandat 4020.066 kb on + strandat 4020.066 kb on + strandat 4020.066 kb on + strandat 4020.067 kb on - strandat 4020.067 kb on - strandat 4020.067 kb on - strandat 4020.072 kb on + strandat 4020.072 kb on + strandat 4020.072 kb on + strandat 4020.073 kb on - strandat 4020.073 kb on - strandat 4020.073 kb on - strandat 4020.073 kb on - strandat 4020.073 kb on - strandat 4020.125 kb on + strandat 4020.126 kb on - strandat 4020.126 kb on - strandat 4020.126 kb on - strandat 4020.126 kb on - strandat 4020.126 kb on - strandat 4020.205 kb on + strand, within Pf1N1B4_3609at 4020.206 kb on - strand, within Pf1N1B4_3609at 4020.206 kb on - strand, within Pf1N1B4_3609at 4020.206 kb on - strand, within Pf1N1B4_3609at 4020.262 kb on + strand, within Pf1N1B4_3609at 4020.262 kb on + strand, within Pf1N1B4_3609at 4020.263 kb on - strand, within Pf1N1B4_3609at 4020.263 kb on - strand, within Pf1N1B4_3609at 4020.263 kb on - strand, within Pf1N1B4_3609at 4020.263 kb on - strand, within Pf1N1B4_3609at 4020.442 kb on + strandat 4020.747 kb on + strand, within Pf1N1B4_3610at 4020.748 kb on - strand, within Pf1N1B4_3610at 4020.852 kb on + strand, within Pf1N1B4_3610at 4020.852 kb on + strand, within Pf1N1B4_3610at 4020.853 kb on - strand, within Pf1N1B4_3610at 4020.853 kb on - strand, within Pf1N1B4_3610at 4020.853 kb on - strand, within Pf1N1B4_3610at 4020.937 kb on - strand, within Pf1N1B4_3610at 4020.951 kb on + strand, within Pf1N1B4_3610at 4021.084 kb on - strand, within Pf1N1B4_3610at 4021.084 kb on - strand, within Pf1N1B4_3610at 4021.084 kb on - strand, within Pf1N1B4_3610at 4021.084 kb on - strand, within Pf1N1B4_3610at 4021.084 kb on - strand, within Pf1N1B4_3610at 4021.151 kb on + strand, within Pf1N1B4_3610at 4021.152 kb on - strand, within Pf1N1B4_3610at 4021.152 kb on - strand, within Pf1N1B4_3610at 4021.152 kb on - strandat 4021.152 kb on - strand, within Pf1N1B4_3610

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Per-strain Table

Position Strand Gene LocusTag Fraction Spectinomycin 0.025 mg/ml
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4,019,231 + Pf1N1B4_3607 0.85 -1.3
4,019,231 + Pf1N1B4_3607 0.85 +1.5
4,019,279 + Pf1N1B4_3607 0.89 -2.3
4,019,279 + Pf1N1B4_3607 0.89 +0.2
4,019,280 - Pf1N1B4_3607 0.89 +0.7
4,019,280 - Pf1N1B4_3607 0.89 -0.8
4,019,342 + -0.9
4,019,342 + -0.6
4,019,342 + -0.3
4,019,342 + -0.5
4,019,343 - -0.9
4,019,343 - -0.9
4,019,343 - -0.3
4,019,343 - -0.9
4,019,343 - +0.2
4,019,343 - -0.6
4,019,343 - +1.0
4,019,343 - -0.3
4,019,589 - Pf1N1B4_3608 0.34 +0.3
4,019,719 + Pf1N1B4_3608 0.62 +0.1
4,019,720 - Pf1N1B4_3608 0.63 -0.2
4,019,882 + +0.4
4,019,934 - +0.7
4,019,980 - +1.0
4,020,066 + -0.8
4,020,066 + -0.9
4,020,066 + -0.9
4,020,067 - -0.1
4,020,067 - +0.3
4,020,067 - +1.3
4,020,072 + -0.7
4,020,072 + +0.1
4,020,072 + +0.1
4,020,073 - -0.3
4,020,073 - -1.5
4,020,073 - -0.5
4,020,073 - +0.2
4,020,073 - -0.3
4,020,125 + +0.3
4,020,126 - -0.9
4,020,126 - +1.2
4,020,126 - +0.2
4,020,126 - -1.9
4,020,126 - -0.7
4,020,205 + Pf1N1B4_3609 0.32 -1.9
4,020,206 - Pf1N1B4_3609 0.33 -1.3
4,020,206 - Pf1N1B4_3609 0.33 -2.1
4,020,206 - Pf1N1B4_3609 0.33 +0.7
4,020,262 + Pf1N1B4_3609 0.51 -2.1
4,020,262 + Pf1N1B4_3609 0.51 -1.2
4,020,263 - Pf1N1B4_3609 0.51 +0.3
4,020,263 - Pf1N1B4_3609 0.51 -0.2
4,020,263 - Pf1N1B4_3609 0.51 +0.3
4,020,263 - Pf1N1B4_3609 0.51 -0.3
4,020,442 + -0.6
4,020,747 + Pf1N1B4_3610 0.19 +0.7
4,020,748 - Pf1N1B4_3610 0.19 -0.1
4,020,852 + Pf1N1B4_3610 0.26 -0.4
4,020,852 + Pf1N1B4_3610 0.26 +0.4
4,020,853 - Pf1N1B4_3610 0.26 +0.7
4,020,853 - Pf1N1B4_3610 0.26 +0.1
4,020,853 - Pf1N1B4_3610 0.26 +0.1
4,020,937 - Pf1N1B4_3610 0.31 +0.9
4,020,951 + Pf1N1B4_3610 0.31 -0.6
4,021,084 - Pf1N1B4_3610 0.39 -2.3
4,021,084 - Pf1N1B4_3610 0.39 +2.7
4,021,084 - Pf1N1B4_3610 0.39 -1.7
4,021,084 - Pf1N1B4_3610 0.39 -0.9
4,021,084 - Pf1N1B4_3610 0.39 -0.7
4,021,151 + Pf1N1B4_3610 0.43 +1.2
4,021,152 - Pf1N1B4_3610 0.43 +1.1
4,021,152 - Pf1N1B4_3610 0.43 -0.9
4,021,152 - +0.1
4,021,152 - Pf1N1B4_3610 0.43 -0.3

Or see this region's nucleotide sequence